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The effect of physical factors on the rate of respiration of the infective larvae of Strongyloides ratti Sandground, 1925

Published online by Cambridge University Press:  06 April 2009

J. Barrett
J. Barrett
Affiliation:
The Molteno Institute, University of Cambridge
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Extract

Dense larval suspensions have a higher Qo2 than dilute ones, probably due to an oxygen debt acquired during preliminary washings. Ammonia (0·1M) did not affect the respiratory rate of the infective larvae of S. ratti. Mechanical agitation of larvae reduced their rate of respiration.

The partial pressure: respiration curve of the infective larvae was sigmoidal, that of larval homogenates was a rectangular hyperbola. At low partial pressures of oxygen the respiratory rate depends to a certain extent on the rate of change of the partial pressure.

In 100% oxygen the respiratory rate of the larvae is at first stimulated and then falls to its initial level. On returning to air after 100% oxygen, the respiratory rate is at first depressed and then recovers.

The infective larvae of S. ratti develop an oxygen debt when kept under anaerobic conditions. During the recovery period, which lasts for about 1 h, the Qo2 may increase by up to 100%.

The respiration of the infective larvae increases as the temperature is increased. When larvae are maintained at 37 °C there is a gradual decrease in their rate of respiration and this change is apparently irreversible.

The rate of respiration of the larvae is slightly inhibited at low pH values and slightly stimulated at high pH values.

Moderate levels of carbon dioxide (5%) have no effect on the respiratory rate of the infective larvae of S. ratti, high levels of carbon dioxide (10–20%), have a definite narcotic effect on the larvae.

The respiration of the infective larvae is unaffected by osmotic pressures up to an equivalent of 1% sodium chloride. Above this level the respiratory rate is at first stimulated then decreases.

This work was carried out during the tenure of a Medical Research Council Scholarship.

Type
Research Article
Information
Parasitology , Volume 59 , Issue 4 , November 1969 , pp. 859 - 875
Copyright
Copyright © Cambridge University Press 1969

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