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Effect of acute phase proteins, especially α2-macroglobulin, on granuloma formation around Schistosoma mansoni eggs in the rat

Published online by Cambridge University Press:  06 April 2009

J. Van Gool ,
D. Van Tiel ,
M. J. Doenhoff  and
H. Van Vugt
J. Van Gool
Affiliation:
Laboratory of Experimental Medicine, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
D. Van Tiel
Affiliation:
Laboratory of Experimental Medicine, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
M. J. Doenhoff
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK
H. Van Vugt
Affiliation:
Laboratory of Experimental Medicine, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
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Summary

A new model for the study of granuloma formation in the liver is described. Rats received an injection of 20 000 Schistosoma mansoni eggs in the portal vein and granuloma formation was evaluated at 3, 5 and 7 weeks post-injection. Liver collagen was estimated at the same time and serum procollagen III peptide, a marker of collagenesis, weekly. With this model, wherein the number of S. mansoni eggs and the time of injury are standardized, the effect of high levels of acute phase proteins especially α2-macroglobulin on granuloma formation was studied. It appeared that in rats with high levels of α2-macroglobulin the mean size of granulomas was significantly greater at 3 and 5 weeks compared with controls. In both groups an increase in liver collagen was observed during this period, reaching a peak at 5 weeks in the acute phase group. This model facilitates the study of the effects of S. mansoni eggs on granuloma formation.

Keywords

Schistosoma mansoniegg granulomasrat modelα2-macroglobulinacute phase proteins
Type
Research Article
Information
Parasitology , Volume 102 , Issue 1 , February 1991 , pp. 49 - 56
Copyright
Copyright © Cambridge University Press 1991

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