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Distribution and biophysical properties of fluorescent lipids on the surface of adult Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

C. A. Redman  and
J. R. Kusel
C. A. Redman*
Affiliation:
Davidson Building, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ
J. R. Kusel
Affiliation:
Davidson Building, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ
*
*Corresponding author: Tel: 0141 330 6449. Fax: 0141 330 4620. E-mail: gbca-66@udcf.gla.ac.uk (John Kusel)
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Summary

The properties of 4 fluorescent lipid compounds in the surface membrane of adult male Schistosoma mansoni worms were examined by fluorescent microscopy and fluorescent recovery after photobleaching (FRAP). The data suggest that the probes N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl) sphingosine (BODIPY FL ceramide) and PKH2 pass through the outer membrane and enter structures in or below the membrane. In contrast 5-(N-octadecanoyl)aminofluorescein (AF18) and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl) sphingosylphosphocholine (BODIPY FL sphingomyelin) insert into the outer monolayer. The DL values of these latter 2 compounds, 8·83 ± 2·35 × 10−9 cm2 s−1 and 2·76 ± 0·53 × 10−9cm2 s−1, respectively, suggest that they enter different domains. Furthermore, it was observed that both BODIPY FL ceramide and BODIPY FL sphingomyelin entered particular structures in or under the surface membrane. The possible nature of these particles is discussed.

Keywords

Schistsoma mansoniceramidesphingomyelinlateral diffusion
Type
Research Article
Information
Parasitology , Volume 113 , Issue 2 , August 1996 , pp. 137 - 143
Copyright
Copyright © Cambridge University Press 1996

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