Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-24T04:58:26.577Z Has data issue: false hasContentIssue false
  • English
  • Français

Description of Metchnikovella spiralis sp. n. (Microsporidia: Metchnikovellidae), with notes on the ultrastructure of metchnikovellids

Published online by Cambridge University Press:  09 May 2014

Y. Y. SOKOLOVA ,
G. G. PASKEROVA ,
Y. M. ROTARI ,
E. S. NASSONOVA  and
A. V. SMIRNOV
Y. Y. SOKOLOVA*
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia Microscopy Center, Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA
G. G. PASKEROVA
Affiliation:
Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia
Y. M. ROTARI
Affiliation:
Institute of Ecology of the Volga River Basin RAS, Togliatti, Russia
E. S. NASSONOVA
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia
A. V. SMIRNOV
Affiliation:
Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia
*
*Corresponding author: Microscopy Center, Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA. E-mail: sokolova@lsu.edu
Get access Rights & Permissions [Opens in a new window]

Summary

The present paper reports results of a transmission electron microscopy study of a new metchikovellid microsporidium. It was isolated from gregarines Polyrhabdina sp. inhabiting guts of polychaetes Pygospio elegans sampled at the White Sea silt littoral zone. Free sporogony (FS) occurred in the life cycle of the microsporidium alongside sac-bound sporogony (BS). Free spores resided in a parasitophorous vacuole and were of typical metchnikovellidean structure, uninucleate and oblong. They measured on sections 2·0–3·2×1·3–1·9 μm. The life cycle included pre-sporogonial stages represented by dikaryotic cells and 4-nucleate cells with coupled nuclei. A multinucleate sporogonial plasmodium of FS split in numerous (>10) sporoblasts. In BS segregation of sporoblasts occurred within thick-walled cysts by internal budding. Spore sacs of this microsporidium, measuring on average 11·6×4·7 μm, were limited by a thick electron-dense wall, externally ornamented with spirally wound cords of dense material. These oval spore sacs contained eight barrel-shaped spores, comparable in size and ultrastructure to FS spores. Ultrastructure of both types of spores and intracellular development of the new microsporidium and Metchnikovella spp. were similar, suggesting they belong to the same genus. In this paper we describe a new species Metchnikovella spiralis and discuss morphology of metchnikovellids in the context of putative evolutionary history of Microsporidia.

Keywords

MicrosporidiaMetchnikovellidaeintracellular parasiteshyperparasitismpolychaetesgregarinesultrastructurelife cycle
Type
Research Article
Information
Parasitology , Volume 141 , Issue 8 , July 2014 , pp. 1108 - 1122
Check for updates
Copyright
Copyright © Cambridge University Press 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Capella-Gutiérrez, S., Marcet-Houben, M. and Gabaldón, T. (2012). Phylogenomics supports microsporidia as the earliest diverging clade of sequenced fungi. BMC Biology 10, 47. doi: 10.1186/1741-7007-10-47.CrossRefGoogle ScholarPubMed
Caullery, M. and Mesnil, F. (1897). Sur un type nouveau (Metchnikovella n.g.) d'organismes parasites des Grégarines. C. R. Soc. Biol. 125, 787–790.Google Scholar
Caullery, M. and Mesnil, F. (1914). Sur les Metchnikovellidae et autre Protistes des Grégarines d'Annélides. C. R. Soc. Biol. 77, 527–532.Google Scholar
Caullery, M. and Mesnil, F. (1919). Metchnikovellidae et autre Protistes des Grégarines d'Annélides. Annales de l'Institut Pasteur 33, 209–240.Google Scholar
Desportes, I. and Théodoridès, J. (1979). Étude ultrastructurale d‘Amphiamblys laubieri n. sp. (Microsporidie, Metchnikovellidae) parasite d'un Grégarine (Lecudina sp.) d'un Echiurien abyssal. Protistologica 15, 435–457.Google Scholar
Dogiel, V. A. (1922). Sur un nouveau genre de Metchnikovellidae. Annales de l'Institut Pasteur 36, 574–577.Google Scholar
Hildebrand, H. (1974). Observations ultrastructurales sur le stade plasmodial de Metchnikovella wohlfarthi Hildebrand et Vivier 1971, microsporidie hyperparasite de la grégarine Lecudina tuzetae . Protistologica 10, 5–15.Google Scholar
Hildebrand, H. and Vivier, E. (1971). Observations ultrastructurales sur le sporoblaste de Metchnikovella wohlfarthi, n. sp. (Microsporidies), parasite de la grégarine Lecudina tuzetae . Protistologica 7, 131–139.Google Scholar
International Commission on Zoological Nomenclature (2012). Amendment of Articles 8, 9, 10, 21 and 78 of the International Code of Zoological Nomenclature to expand and refine methods of publication. ZooKeys 219, 1–10.CrossRefGoogle Scholar
Issi, I. V. (1986). Microsporida as a type of parasitic Protozoa. In Microsporidia, Vol. Protozoologiya V.10 (ed. Beyer, T. V. and Issi, I. , V.), pp. 6–136. Nauka, Leningrad, Russia.Google Scholar
Larsson, J. I. R. (1993). Description of Chytridiopsis trichopterae n. sp. (Microspora, Chytidiopsidae), a microsporidian parasite of the caddis fly Polycentropus flavomaculatus (Trichoptera, Polycentropodidae), with comments on relationships between families Chytridiopsidae and Metchnikovellidae. Journal of Eukaryotic Microbiology 40, 37–48. doi: 10.1111/j.1550-7408.1993.tb04880.x.CrossRefGoogle Scholar
Larsson, J. I. R. (2000). The hyperparasitic microsporidium Amphiacantha longa Caullery et Mesnil, 1914 (Microspora: Metchnikovellidae). Description of the cytology, redescription of the species, emended diagnosis of the genus Amphiacantha and establishment of the new family Amphiacanthidae. Folia Parasitologica 47, 241–256.CrossRefGoogle ScholarPubMed
Larsson, R. and Køie, M. (2006). The ultrastructure and reproduction of Amphiamblys capitellides (Microspora, Metchnikovellidae), a parasite of the gregarine Ancora sagittata (Apicomplexa, Lecudinidae), with redescription of the species and comments on the taxonomy. European Journal of Protistology 42, 233–248.CrossRefGoogle ScholarPubMed
Lee, S. C., Corradi, N., Byrnes Iii, E. J., Torres-Martinez, S., Dietrich, F. S., Keeling, P. J. and Heitman, J. (2008). Microsporidia evolved from ancestral sexual fungi. Current Biology 18, 1675–1679.CrossRefGoogle ScholarPubMed
Ormières, R., Loubès, C. and Maurand, J. (1981). Amphyamblys bhatiellae n. sp., microsporidie parasite de Bhatiella marphysae Setna, 1931, Eugrégarine d'Annélide Polychète. Protistologica 17, 273–280.Google Scholar
Redhead, S. A., Kirk, P. M., Keeling, P. J. and Weiss, L. M. (2009). (048-051) Proposals to exclude the phylum Microsporidia from the code. Taxon 58, 658–672.CrossRefGoogle Scholar
Rotari, Y. M. (1988). Microsporidia – hyperparasites of the White Sea polychaetes. MS dissertation. Biological Sci., Dept. Invertebrate Zoology, Leningrad State University, Leningrad.Google Scholar
Rotari, Y. and Paskerova, G. (2007). New data on hyperparasitic microsporidians from polychaetes of the White Sea. In Proceedings of the V European Congress of Protistology and XI European Conference on Ciliate Biology, St. Petersburg. Protistology 5, 67–68.Google Scholar
Sokolova, Y. Y. and Fuxa, J. R. (2008). Biology and life-cycle of the microsporidium Kneallhazia solenopsae Knell Allan Hazard 1977 gen. n., comb. n., from the fire ant Solenopsis invicta . Parasitology 135, 903–929.CrossRefGoogle Scholar
Sokolova, Y. Y., Paskerova, G. G., Rotari, Y. M., Nassonova, E. S. and Smirnov, A. V. (2013). Fine structure of Metchnikovella incurvata Caullery and Mesnil 1914 (microsporidia), a hyperparasite of gregarines Polyrhabdina sp. from the polychaete Pygospio elegans . Parasitology 140, 855–867.CrossRefGoogle ScholarPubMed
Sprague, V. (1977). Classification and phylogeny. In Comparative Pathobiology, Vol. 2. Systematics of the Microsporidia (ed. Bulla, L. A. and Cheng, T. C.), pp. 1–30. Plenum Press, New York, NY, USA.Google Scholar
Sprague, V., Becnel, J. J. and Hazard, E. I. (1992). Taxonomy of Phylum Microspora. Critical Reviews in Microbiology 18, 285–395.CrossRefGoogle ScholarPubMed
Vivier, E. (1975). The Microsporidia of the Protozoa. Protistologica 11, 345–361.Google Scholar
Vivier, E. and Schrével, J. (1973). Étude en microscopie photonique et électronique de différents stades du cycle de Metchnikovella hovassei et observations sur la position systématique des Metchnikovellidae. Protistologica 9, 95–118.Google Scholar