Hostname: page-component-848d4c4894-mwx4w Total loading time: 0 Render date: 2024-06-24T00:50:23.951Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparison of surface iodination methods by electron microscopic autoradiography applied in vitro to different life-stages of Dipetalonema vitae (Filarioidea)

Published online by Cambridge University Press:  06 April 2009

W. Baschong  and
W. Rudin
W. Baschong
Affiliation:
Swiss Tropical Institute, CH-4051 Basel, Switzerland
W. Rudin
Affiliation:
Swiss Tropical Institute, CH-4051 Basel, Switzerland
Get access Rights & Permissions [Opens in a new window]

Extract

Different stages of Dipetalonema viteae (males, females, microfilariae, and 3rd-stage larvae) have been iodinated in vitro under physiological conditions by chloroglycoluril, lactoperoxidase or chloramine T. The concentrations of the catalysts were correlated with the viability of the worms. Localization of the label with the different iodination methods had been visualized by electron microscopical autoradiography. Chloroglycoluril-mediated iodination is predominantly localized on the filarial cuticle. Lactoperoxidase-catalysed iodination is less specific and chloramine T catalyses iodination in a gradient decreasing from the cuticle to inner structures. It is necessary to visualize the labelling by electron microscopical autoradiography prior to biochemical and immunological experiments to avoid the extraction of structures iodinated by leakage of the catalyst into sub-cuticular regions.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 3 , December 1982 , pp. 559 - 565
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baschong, W., Tanner, M., Betschart, B., Rudin, W. & Weiss, N. (1982). Dipetalonema viteae (Filarioidea): Extraction and immunogenicity of cuticular antigens from adult female worms. Journal of Experimental Parasitology 53, 262–9.Google Scholar
Bienz, K. A. (1977). Techniques and applications of autoradiography in the light and electron microscope. Microscopica Acta 79, 1–22.Google Scholar
Bird, A. F. (1980). The nematode cuticle and its surface. In Nematodes as Biological Models, vol. 2 (ed. Zuckermann, B. M.), pp. 213–36. New York and London: Academic Press.Google Scholar
Forsyth, K. P., Copeman, D. B., Abbot, R. F., Andress, R. F. & Mitchell, G. F. (1981). Identification of radioiodinated cuticular proteins and antigens of Onchocerca gibsoni microfilariae. Acta Tropica 38, 329–42.Google Scholar
Fraker, P. J. & Speck, J. C. jr. (1978). Protein and cell membrane iodinations with a sparingly soluble chloramide, l,3,4,6-tetrachloro-3α,6α-diphenylglycoluril. Biochemical and Biophysical Research Communications 80, 849–57.Google Scholar
Gass, R. F., Tanner, M. & Weiss, N. (1979). Development of Dipetalonema viteae third stage larvae (Nematoda; Filarioidea) in micropore chambers implanted into jirds, hamsters, normal and immunized mice. Zeitschrift für Parasitenkunde 61, 73–82.CrossRefGoogle ScholarPubMed
Hayunga, E. G., Murell, K. D., Taylor, D. W. & Vannier, W. E. (1979). Isolation and characterization of surface antigens from Schistosoma mansoni. I. Evaluation of techniques for radioisotope labelling of surface proteins from adult worms. Journal of Parasitology 65, 488–96.CrossRefGoogle ScholarPubMed
Kopriwa, B. M. (1975). A comparison of various procedures for fine grain development in electron microscopic radioautography. Histochemie 44, 201–24.CrossRefGoogle ScholarPubMed
Lumsden, D. L. (1975). Surface ultrastucture and cytochemistry of parasitic helminths. Experimental Parasitology 37, 267–339.CrossRefGoogle Scholar
Markwell, M. A. K. & Fox, C. F. (1978). Surface specific iodination of membrane proteins of viruses and eucaryotic cells using l,3,4,6-tetrachloro-3α,6α-diphenylglycoluril. Biochemistry 17, 4807–17.Google Scholar
McConahey, P. J. & Dixon, F. J. (1980). Radioiodination of proteins by use of the chloramine T method. In Methods of Enzymology, vol. 70 (ed. van Vunakis, H. and Langone, J. J.), pp. 201–13. New York, London, Toronto, Sydney and San Francisco: Academic Press.Google Scholar
Mackenzie, C. D., Preston, P. M. & Ogilvie, B. M. (1978). Immunological properties of the surface of parasitic nematodes. Nature, London 276, 826–8.Google Scholar
Morrison, M. (1980). Lactoperoxidase catalyzed iodination as a tool for investigation of proteins. In Methods of Enzymology, vol. 70 (ed.van Vunakis, H. and Langone, J. J.), pp. 214–20. New York, London, Toronto, Sydney and San Francisco: Academic Press.Google Scholar
Ogilvie, B. M., Philipp, M., Jungery, M., Maizels, R. M., Worms, M. J. & Parkhouse, R. M. E. (1981). The surface of nematodes and the immune response of the host. In Proceedings of the 3rd Janssen Symposium, Biochemistry of Parasites and Host–Parasite Relationships (ed. Van der Bosch, M.), pp. 99–104. Amsterdam: Elsevier.Google Scholar
Parkhouse, R. M. E., Philipp, M. & Ogilvie, B. M. (1981). Characterization of surface antigens of Trichinella spiralis infective larvae. Parasite Immunology 3, 339–52.Google Scholar
Philipp, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1980). Changing proteins on the surface of a parasitic nematode. Nature, London 287, 538–40.Google Scholar
Phillips, D. R. & Morrison, M. (1971). Exposed protein on the intact Human erythrocyte. Biochemistry 10, 1766–71.Google ScholarPubMed
Rudin, W., Tanner, M., Bauer, P. & Weiss, N. (1980). Studies on Dipetalonema viteae (Filarioidea). 5. Ultrastructural aspects of the antibody-dependent cell-mediated destruction of microfilariae. Tropenmedizin und Parasitologie 31, 194–200.Google Scholar
Schlager, S. I. (1979). Specific 125I-iodination of cell surface lipids: Plasma membrane alterations induced during humorale immune attack. Journal of Immunology 123, 2108–13.CrossRefGoogle Scholar
Weiss, N. & Tanner, M. (1979). Studies on Dipetalonema viteae (Filarioidea). 3. Antibody-dependent cell mediated destruction of microfilariae in vivo. Tropenmedizin und Parasitologie 30, 73–80.Google Scholar
Weiss, N. & Tanner, M. (1981). Experimental filariasis in the Syrian hamster; immunological aspects of complex host-parasite interactions. In Hamster Immune Responses in Infectious and Oncologic Diseases (ed. Wayne Streilien, J.Hart, D. A.Stein-Strelien, J.Duncan, W. R. and Billingham, R. E.), pp. 253–265. New York: Plenum Publishing Corporation.Google Scholar
Worms, M. J., Terry, R. J. & Terry, A. (1961). Dipetalonema witei, filarial parasite of the jird, Meriones libycus. I. Maintenance in the laboratory. Journal of Parasitology 47, 963–70Google Scholar