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Characterization of excretory–secretory products from protoscoleces of Echinococcus granulosus and evaluation of their potential for immunodiagnosis of human cystic echinococcosis

Published online by Cambridge University Press:  23 August 2004

D. CARMENA ,
J. MARTÍNEZ ,
A. BENITO  and
J. A. GUISANTES
D. CARMENA
Affiliation:
Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, P.O. Box 450, 01080 Vitoria, Spain Present address: Department of Biological Sciences, Imperial College London, Biochemistry Building, South Kensington Campus, London SW7 2AZ, UK.
J. MARTÍNEZ
Affiliation:
Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, P.O. Box 450, 01080 Vitoria, Spain
A. BENITO
Affiliation:
Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, P.O. Box 450, 01080 Vitoria, Spain
J. A. GUISANTES
Affiliation:
Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, P.O. Box 450, 01080 Vitoria, Spain
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Abstract

This study describes, for the first time, the characterization of excretory–secretory antigens (ES-Ag) from Echinococcus granulosus protoscoleces, evaluating their usefulness in the immunodiagnosis of human cystic echinococcosis. ES-Ag were obtained from the first 50 h maintenance of protoscoleces in vitro. This preparation contained over 20 major protein components which could be distinguished by 1-dimensional SDS–PAGE with apparent masses between 9 and 300 kDa. The culture of of protoscoleces from liver produced a greater variety of excretory–secretory protein components than those from lung. Determination of enzymatic activities of secreted proteins revealed the presence of phosphatases, lipases and glucosidases, but no proteases. These findings were compared to those obtained from somatic extracts of protoscoleces and hydatid cyst fluid products. Immunochemical characterization was performed by immunoblotting with sera from individuals infected by cystic echinococcosis (n=15), non-hydatidic parasitoses (n=19), various liver diseases (n=24), lung neoplasia (n=16), and healthy donors (n=18). Antigens with apparent masses of 89, 74, 47/50, 32, and 20 kDa showed specificity for immunodiagnosis of human hydatidosis. The 89 and 74 kDa components corresponded to antigens not yet described in E. granulosus, whereas proteins of 41–43 kDa and 91–95 kDa were recognized by the majority of the non-hydatid sera studied.

Keywords

Echinococcus granulosusexcretory–secretory antigensantigenic characterizationprotoscoleceshuman cystic echinococcosisimmunodiagnosis
Type
Research Article
Information
Parasitology , Volume 129 , Issue 3 , September 2004 , pp. 371 - 378
Copyright
© 2004 Cambridge University Press

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