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Carbohydrate/lectin interactions between the nematophagous fungus, Arthrobotrys oligospora, and the infective juveniles of Trichostrongylus colubriformis (Nematoda)

Published online by Cambridge University Press:  06 April 2009

D. A. Wharton  and
D. S. Murray
D. A. Wharton
Affiliation:
Department of Zoology, University of Otago, P.O.Box 56, Dunedin, New Zealand
D. S. Murray
Affiliation:
Department of Zoology, University of Otago, P.O.Box 56, Dunedin, New Zealand
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Summary

Removal of the sheath of the ensheathed infective juvenile of Trichostrongylus colubriformis prevents capture by the nematophagous fungus Arthrobotrys oligospora. Exposure of the trap hyphae to a variety of saccharides, which may block a recognition system based on lectin/carbohydrate binding, failed to prevent capture but some saccharides did inhibit penetration and invasion by the fungus. Capture and penetration thus appear to be two distinct processes with capture being less specific than penetration. Carbohydrate residues were absent from the outer surface of the cuticle and the sheath but were present on the inner surface of the sheath. The limited accessibility of these lectin-binding sites may explain the slow process of infection of the infective juvenile by the fungus. The sheath does not protect the infective juvenile against attack by this nematophagous fungus.

Keywords

Arthrobotrys oligosporaTrichostrongylus colubriformisnematophagous fungustrichostrongyle nematodesheathbiological controllectin/carbohydrate binding
Type
Research Article
Information
Parasitology , Volume 101 , Issue 1 , August 1990 , pp. 101 - 106
Copyright
Copyright © Cambridge University Press 1990

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References

REFERENCES

Biely, P., Kraky, Z., Kovarik, J. & Bauer, S. (1971). Effect of 2-deoxyglucose on cell wall formation in Saccharomyces cerevisiae and its relation to cell growth inhibition. Journal of Bacteriology 107, 121–9.CrossRefGoogle ScholarPubMed
Bone, L. W. & Bottjer, K. P. (1985). Cuticular carbohydrates of three nematode species and chemoreception by Trichostrongylus colubriformis. Journal of Parasitology 71, 235–8.CrossRefGoogle ScholarPubMed
Borrebaeck, L. A. K., Mattiasson, B. & Nordbring-Hertz, B. (1985). A fungal lectin and its apparent receptors on a nematode surface. FEMS Microbiology Letters 27, 35–9.CrossRefGoogle Scholar
Hashmi, H. A. & Connan, R. M. (1989). Biological control of ruminant trichostrongylids by Arthrobotrys oligospora, a predacious fungus. Parasitology Today 5, 28–30.CrossRefGoogle ScholarPubMed
Jansson, H. B. (1987). Receptors and recognition in nematodes. In Vistas on Nematology (ed. Veech, J. A. & Dickson, D. W.). Hyattsville, Maryland: Society of Nematologists Inc.Google Scholar
Milner, A. R. & Mack, W. N. (1988). Trichostrongylus colubriformis: analysis of monoclonal antibodies and lectin binding to the larval cuticle. Parasite Immunology 10, 425–32.CrossRefGoogle Scholar
Murray, D. S. & Wharton, D. A. (1990). Capture and penetration processes of the free-living juveniles of Trichostrongylus colubriformis (Nematoda) by the nematophagous fungus, Arthrobotrys oligospora. Parasitology 101, 93–100.CrossRefGoogle ScholarPubMed
Nansen, P., Gronvold, J., Henriksen, A. & Wolstrup, J. (1986). Predacious activity of the nematode-destroying fungus, Arthrobotrys oligospora, on preparasitic larvae of Cooperia oncophora and on soil nematodes. Proceedings of the Helminthological Society of Washington 53, 237–43.Google Scholar
Nordbring-Hertz, B., Friman, E. & Mattiasson, B. (1982). A recognition mechanism in the adhesion of nematodes to nematode-trapping fungi. In Lectins-Biology, Biochemistry, Clinical Biochemistry, vol. 2 (ed. Bog-Hansen, T. C.). Berlin: Walter de Gruyter.Google Scholar
Nordbring-Hertz, B. & Mattiasson, B. (1979). Action of nematode-trapping fungus shows lectin-mediated host-microorganism interaction. Nature, London 281, 477–9.CrossRefGoogle Scholar
Premachandran, D. & Pramer, D. (1984). Role of N-acetyl-galactosamine-specific protein in trapping of nematodes by Arthrobotrys oligospora. Applied and Environmental Microbiology 47, 1358–9.CrossRefGoogle Scholar
Rosenzweig, W. D. & Ackroyd, D. (1983). Binding characteristics of lectins involved in the trapping of nematodes by fungi. Applied and Environmental Microbiology 46, 1093–6.CrossRefGoogle ScholarPubMed
Rosenzweig, W. D., Premachandran, D. & Pramer, D. (1985). Role of trap lectins in the specificity of nematode capture by fungi. Canadian Journal of Microbiology 31, 693–5.CrossRefGoogle Scholar
Wharton, D. A. (1986 a). A Functional Biology of Nematodes. London and Sydney: Croom Helm.CrossRefGoogle Scholar
Wharton, D. A. (1986 b). The structure of the cuticle and sheath of the infective juvenile of Trichostrongylus colubriformis. Parasitology Research 72, 779–87.Google ScholarPubMed
Wharton, D. A. & Murray, D. S. (1989). 3D reconstruction from optical sections of the nematode-trapping fungus, Arthrobotrys oligospora. Journal of Microscopy 154, 157–63.CrossRefGoogle Scholar
Wharton, D. A. & Sommerville, R. I. (1984). The structure of the excretory system of the infective larva of Haemonchus contortus. International Journal for Parasitology 14, 591–600.CrossRefGoogle ScholarPubMed