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Analysis of the high molecular weight rhoptry complex of Plasmodium falciparum using monoclonal antibodies

Published online by Cambridge University Press:  06 April 2009

J.-C. Doury ,
S. Bonnefoy ,
N. Roger ,
J.-F. Dubremetz  and
O. Mercereau-Puijalon
J.-C. Doury
Affiliation:
Unité de Biologie Parasitaire, Institut de Médecine Tropicale, Parc du Pharo, 13998 Marseille Armées, France
S. Bonnefoy
Affiliation:
Unité de Parasitologie expérimentale, Institut Pasteur, 25 Rue du Docteur Roux, 75015 Paris, France
N. Roger
Affiliation:
Unité 42 INSERM, 369 rue Jules Guesde, 59650 Villeneuve d'Ascq, France
J.-F. Dubremetz
Affiliation:
Unité de Parasitologie expérimentale, Institut Pasteur, 25 Rue du Docteur Roux, 75015 Paris, France
O. Mercereau-Puijalon
Affiliation:
Unité de Parasitologie expérimentale, Institut Pasteur, 25 Rue du Docteur Roux, 75015 Paris, France
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Summary

Twenty-one monoclonal antibodies, obtained after immunization of mice with erythrocytic stages of Plasmodium falciparum, produced a double dot image in IFA. Immunoelectronmicroscopy indicated that the mAbs reacted with the rhoptries. Rhoptries are pear-shaped apical organelles, believed to be involved in invasion of the host cell by the parasite. The mAbs all immunoprecipitated the high molecular weight antigen complex. Some mAbs recognized on immunoblots only 1 protein of this complex, whereas others reacted with RhopH1 and RhopH3, or RhopH2 and RhopH3 or with the 3 proteins. An additional antigen of 52 kDa was also recognized by some of the mAbs. The epitopes defined by the mAbs were present in most of the 40 P. falciparum strains or isolates studied by IFA. Interestingly, the mAbs also reacted with high titres on P. vivax and P. ovale, but produced images that did not indicate an apical location. The mAbs failed to react on the non-human malaria parasites studied, P. cynomolgi and P. inui. On P. berghei or P. chabaudi parasites, only 5 mAbs gave a positive reaction, labelling a large network outside the parasite. Finally, the mAbs did not react with P. falciparum sporozoites, indicating that the rhoptries of merozoites and sporozoites, the two invasive stages of the malaria life-cycle are equipped with distinct sets of proteins.

Keywords

Apicomplexarhoptriesepitopescell invasionmonoclonal antibodies
Type
Research Article
Information
Parasitology , Volume 108 , Issue 3 , April 1994 , pp. 269 - 280
Copyright
Copyright © Cambridge University Press 1994

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