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An examination of the skin phase of schistosome migration using a hamster cheek pouch preparation

Published online by Cambridge University Press:  06 April 2009

R. A. Wilson  and
J. Ruth Lawson
R. A. Wilson
Affiliation:
Department of Biology, University of York, Heslington, York
J. Ruth Lawson
Affiliation:
Department of Biology, University of York, Heslington, York
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Summary

A living hamster cheek pouch preparation has been used to study the skin phase of schistosomulum migration. Between 0 and 16 h post-exposure schistosomula become increasingly difficult to see and lateral movement in the skin ceases. Up to 50 h post-exposure they remain poorly visible. They lie in fluid-filled lacunae within the epidermis, external to the basement membrane. The first schistosomula enter the dermis from. 40 h post-exposure, approximately half having done so by 52·5 h. Coincidentally, they can be more easily distinguished from surrounding tissue. Up to 91% of original penetrants have been observed alive in. pouch tissue at 65 h post-exposure. Since only 26% eventually mature in the hepatic portal system there would appear to be considerable attrition subsequent to the skin phase of migration. The mean diameter of venules penetrated was 22·7 μm and the mean time taken to penetrate was 8·01 h. The majority of schistosomula left the pouch between 60 and 80 h post-exposure.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 2 , April 1980 , pp. 257 - 266
Copyright
Copyright © Cambridge University Press 1980

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References

Billengham, R. E. & Silvers, W. K. (1962). Studies on pouch skin homografts in the Syrian hamster. In Ciba Symposium on Transplantation (ed. Wolstenholme, G. E. W. and Cameron, M. P.). Boston: Little Brown and Company.Google Scholar
Clegg, J. A. & Smithers, S. R. (1968). Death of schistosome cercariae during penetration of the skin. II. Penetration of mammalian skin by Schistosoma mansoni. Parasitology 58, 111–28.CrossRefGoogle ScholarPubMed
Ghandour, A. M. & Ibrahim, A. M. (1978). A study of the relationship between the energy content of Schistosoma mansoni cercariae and their death during penetration of mammalian host skin. Journal of Helminthology 52, 339–42.CrossRefGoogle ScholarPubMed
Ghandour, A. M. & Webbe, G. (1973). A study of the death of Schistosoma mansoni cercariae during penetration of mammalian host skin: the influence of the ages of cercariae and of the host. International Journal for Parasitology 3, 789–94.CrossRefGoogle ScholarPubMed
Gordon, R. M. & Griffiths, R. B. (1951). Observations on the means by which the cercariae of Schistosoma mansoni penetrate mammalian skin, together with an account of certain morphological changes observed in the newly penetrated larvae. Annals of Tropical Medicine and Parasitology 45, 227–43.CrossRefGoogle ScholarPubMed
Hitchcock, D. J. (1949). Penetration characteristics of Schistosoma mansoni cercariae. Journal of Parasitology 35, 216–17.CrossRefGoogle ScholarPubMed
Lewert, R. M. & Mandlowitz, S. (1963). Innate immunity to Schistosoma mansoni relative to the state of connective tissue. Annals of the New York Academy of Sciences 113, 54–62.CrossRefGoogle Scholar
von Lichtenberg, F., Sher, A., Gibbons, N. & Doughty, B. L. (1976). Eosinophil enriched inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni. American Journal of Pathology 84, 479–500.Google Scholar
Miller, P. & Wilson, R. A. (1978). Migration of the schistosomula of Schistosoma mansoni from skin to lungs. Parasitology 77, 281–302.CrossRefGoogle ScholarPubMed
Miller, P. & Wilson, R. A. (1980). Migration of the schistosomula of Schistosoma mansoni from the lungs to hepatic portal system. Parasitology 80, 267–88.CrossRefGoogle ScholarPubMed
Pantin, C. F. A. (1964). Notes on Microscopical Technique for Zoologists. Cambridge: Cambridge University Press.Google Scholar
Sher, A., Mackenzie, P. & Smithers, S. R. (1974). Decreased recovery of invading parasites from the lungs as a parameter of acquired immunity to schistosomiasis in the mouse. Journal of Infectious Diseases 130, 626–32.CrossRefGoogle ScholarPubMed
Stirewalt, M. A. (1959). Chronological analysis, pattern and rate of migration of cercariae of Schistosoma mansoni in body, ear and tail skin of mice. Annals of Tropical Medicine and Parasitology 53, 400–13.CrossRefGoogle ScholarPubMed
Stirewalt, M. A. & Hackey, J. R. (1956). Penetration of host skin by cercariae of Schistosoma mansoni. 1. Observed entry into skin of mouse, hamster, rat, monkey and man. Journal of Parasitology 42, 565–80.CrossRefGoogle Scholar
Stirewalt, M. A. & Kruidenier, F. J. (1961). Activity of the acetabular secretory apparatus of cercariae of Schistosoma mansoni under experimental conditions. Experimental Parasitology 11, 191–211.CrossRefGoogle ScholarPubMed
Wheater, P. R. & Wilson, R. A. (1979). Schistosoma mansoni: a histological study of migration in the laboratory mouse. Parasitology 79, 49–62.CrossRefGoogle ScholarPubMed