Hostname: page-component-848d4c4894-sjtt6 Total loading time: 0 Render date: 2024-06-20T02:58:28.512Z Has data issue: false hasContentIssue false
  • English
  • Français

Active entry of bloodstream forms of Trypanosoma cruzi into macrophages

Published online by Cambridge University Press:  06 April 2009

T. L. Kipnis ,
V. L. G. Calich  and
W. Dias da Silva
T. L. Kipnis
Affiliation:
Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Univereidade de São Paulo, São Paulo, Brasil
V. L. G. Calich
Affiliation:
Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Univereidade de São Paulo, São Paulo, Brasil
W. Dias da Silva
Affiliation:
Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Univereidade de São Paulo, São Paulo, Brasil
Get access Rights & Permissions [Opens in a new window]

Summary

The uptake of bloodstream forms of Trypanosoma cruzi, Y and CL stocks, by mouse peritoneal macrophages and their intracellular differentiation and multiplication has been compared in vitro. After 48 h the number of macrophages showing intracellular amastigote forms was higher when the Y stock was used. The number of parasitized cells increased with the time of contact between parasites and macrophages. Prior treatment of the parasites with anti-T. cruzi antibodies and/or complement increased the number of infected macrophages, but did not interfere with their subsequent differentiation within the macrophages. The number of parasitized cells was greater when macrophages were obtained from mice previously treated with lipopolysaccharide, peptone or thioglycollate. Uptake was not appreciably affected when macrophages were pre-treated with trypsin or anti-macrophage serum, or when the parasites and macrophages were incubated in the presence of cytochalasin B. In the same experimental conditions, epimastigotes of T. cruzi were not able to differentiate into amastigotes. Their uptake was potentiated by previous treatment with specific antibodies and/or complement and was blocked by cytochalasin B. These results confirm that epimastigotes derived from T. cruzi cultures are phagocytosed and suggest that bloodstream forms penetrate actively into macrophages.

Type
Research Article
Information
Parasitology , Volume 78 , Issue 1 , February 1979 , pp. 89 - 98
Copyright
Copyright © Cambridge University Press 1979

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alexander, J. (1975). Effect of the antiphagocytic agent cytochalasin B on macrophage invasion by Leishmania mexicana promastigotes and Trypanosoma cruzi epimastigotes. Journal of Protozoology 22, 237–40.CrossRefGoogle ScholarPubMed
Allison, A. C., Davies, P. & de Petris, S. (1971). Role of contractile microfilaments in macrophage movement and endocytosis. Nature, New Biology 232, 153–5.CrossRefGoogle ScholarPubMed
Bianco, C., Griffin, F. M. & Silverstein, S. C. (1975). Studies of macrophage complement receptors: alteration of receptor function upon macrophage activation. Journal of Experimental Medicine 141, 1278–90.CrossRefGoogle ScholarPubMed
Brenner, Z. (1975). Significance of morphologic variation of bloodstream forms.: New approaches in American trypanosomiasis research. Pan American Health Organization. Scientific Publication No. 318, 127–31.Google Scholar
Camargo, E. P. (1964). Growth and differentiation in Trypanosoma cruzi. I. Origin of metacyclic trypanosomes in liquid media. Revista do Instituto de Medicina Tropical, São Paulo 93–100.Google ScholarPubMed
Cappa, S. M. G., Kloetzel, J. & dos Santos, Ribeiro R. (1977). Antibody-induced modulation of Trypanosoma cruzi surface membrane antigens. Pesquisa básica em Doença de Chagas. IV. Reunião Anual, Caxambu, Brasil.Google Scholar
Griffin, F. M. Jr, Griffin, J. A. & Silverstein, S. C. (1976). Studies on the mechanism of phagocytosis. II. The interaction of macrophages with anti-immunoglobulin IgG-coated bone marrow-derived lymphocytes. Journal of Experimental Medicine 144, 788–809.CrossRefGoogle ScholarPubMed
Holland, P., Holland, N. H. & Cohn, Z. A. (1972). The selective inhibition of macrophage phagocytic receptors by antimembrane antibodies. Journal of Experimental Medicine 135, 458–75.CrossRefGoogle Scholar
Kierszenbaum, F., Ivany, J. & Budzko, D. G. (1976). Mechanisms of natural resistance to trypanosomal infection. Role of complement in avian resistance to Trypanosoma cruzi infection. Immunology 30, 1–6.Google ScholarPubMed
Kloetzel, J. & Deane, M. P. (1977). Presence of immunoglobulins on the surface of bloodstream T. cruzi. Capping during differentiation in culture. Revistado Instituto de Medicina Tropical, São Paulo 19, 397–402.Google ScholarPubMed
Krettly, A. U. & Nussenzweig, R. (1977). A surface coat of immunoglobulins on the bloodstream forms of Trypanosoma cruzi. Pesquisa Básica em Doença de Chagas. IV. Reunião Anual Caxambu, Brasil.Google Scholar
Lay, W. H. & Nussenzweig, V. (1968). Receptors for complement on leukocytes. Journal of Experimental Medicine 128, 991–1009.CrossRefGoogle ScholarPubMed
Lay, W. H. & Nussenzweig, V. (1969). Ca++-dependent binding of antigen-19S antibody complexes to macrophages. Journal of Immunology 102, 1172–8.CrossRefGoogle Scholar
Mayer, M. M. (1961). Complement and complement fixation. In Experimental Immunochemistry (ed. Kabat, E. A.) Chapter 4, Springfield, Ill.: Charles C. Thomas.Google Scholar
Nelson, D. S. (1976). Immunobiology of the Macrophage. New York: Academic Press.Google Scholar
Noqueira, N. & Cohn, Z. (1976). Trypanosoma cruzi: mechanism of entry and intracellular fate in mammals cells. Journal of Experimental Medicine 143, 1402–20.CrossRefGoogle Scholar
Rabinovich, M. & DeStefano, M. J. (1973). Macrophage spreading in vitro. I. Inducers of spreading. Experimental Cell Research 77, 323–34.CrossRefGoogle Scholar
Rabinovich, M., Manejias, R. E., Russo, M. & Abbey, E. E. (1976). Increased spreading of macrophages from mice treated with interferon inducers. Cellular Immunology 29, 86–95.CrossRefGoogle Scholar
Schmuñis, G. A., Szarfman, H. & Souza, W. (1977). Antibody-induced modulation of Trypanosoma cruzi surface membrane antigens. Pesquisa Básica em Doença de Chagas. 4. Reunião Anual, Caxambu, Brasil.Google Scholar
Takehara, H. & da Silva, Dias W. (1975). Mechanism of resistance against T. cruzi in animals vaccinated with L. pessoai. New Approaches in American Trypanosomiasis. Pan American Health Organization. Scientific Publication No. 318, 174–8.Google Scholar
Werb, Z., Cohn, Z. A. (1971). Cholesterol metabolism in macrophages. 1. The regulation of cholesterol compartments and exchange in other cell types. Journal of Experimental Medicine 134, 1570–90.CrossRefGoogle Scholar