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Secretory IgA responses in rats to antigens of various developmental stages of Nippostrongylus brasiliensis

Published online by Cambridge University Press:  06 April 2009

H. Wedrychowicz ,
J. M. Maclean  and
P. H. Holmes
H. Wedrychowicz
Affiliation:
Department of Veterinary Physiology, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61
J. M. Maclean
Affiliation:
Department of Veterinary Physiology, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61
P. H. Holmes
Affiliation:
Department of Veterinary Physiology, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61
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Summary

Intestinal and faecal IgA responses against different developmental stages of Nippostrongylus brasiliensis were studied in Hooded Lister rats vaccinated subcutaneously with infective larvae or intragastrically with 4th-stage larvae, adult nematodes, adult worm somatic and metabolic antigens. It was found that intragastric administration of L4 or adult worms produced as high resistance to challenge as normal infection. Oral vaccination with adult nematode excretory–secretory (ES) and somatic antigens produced 69 and 17% protection respectively. All vaccination regimes induced a considerable IgA response against somatic and metabolic antigens of the intestinal stages. However, no correlation could be seen between the IgA levels and degree of protection. Analysis of intestinal mucosal, intestinal contents and faecal IgA specific to larval or adult antigens suggests that the IgA is probably induced by antigen(s) common to all intestinal stages. Also secretory IgA responses against surface antigens of intestinal L4 did not show stage specificity, although this was detected in sera.

Type
Research Article
Information
Parasitology , Volume 89 , Issue 1 , August 1984 , pp. 145 - 158
Copyright
Copyright © Cambridge University Press 1984

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