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Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis

Published online by Cambridge University Press:  27 March 2018

Soon Bin Kwon
Affiliation:
Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul 03722, Korea Vaccine Translational Research Center, Yonsei University, Seoul 03722, Korea
Paul Kim
Affiliation:
Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul 03722, Korea Vaccine Translational Research Center, Yonsei University, Seoul 03722, Korea
Hae Sun Woo
Affiliation:
Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul 03722, Korea
Tae Yun Kim
Affiliation:
Division of Vectors and Parasitic Diseases, Center for Laboratory Control of Infectious Diseases, Korea Center for Disease Control and Prevention, Osong-eup, Cheongju-si, Chungcheongbuk-do 28159, Korea
Ju Yeong Kim
Affiliation:
Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul 03722, Korea
Hye Min Lee
Affiliation:
Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul 03722, Korea Vaccine Translational Research Center, Yonsei University, Seoul 03722, Korea
Yun Soo Jang
Affiliation:
Department of Environmental Medical Biology and Institute of Tropical Medicine, Seoul 03722, Korea
Eun-Min Kim
Affiliation:
Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul 03722, Korea
Tai-Soon Yong
Affiliation:
Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul 03722, Korea
Baik Lin Seong*
Affiliation:
Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul 03722, Korea Vaccine Translational Research Center, Yonsei University, Seoul 03722, Korea
*
Author for correspondence: Baik Lin Seong, E-mail: blseong@yonsei.ac.kr

Abstract

Due to the lack of an effective prophylactic intervention and diagnosis, human liver fluke Clonorchis sinensis continues to afflict a large human population, causing a chronic inflammatory bile duct disease. With an aim to identify target antigens for sensitive serodiagnosis, adenylate kinase 3 of C. sinensis (CsAK3) was successfully expressed in soluble form in Escherichia coli by fusion to an RNA-interacting domain derived from human Lys-tRNA synthetase and purified by Ni2+-affinity chromatography. Anti-CsAK3 serum was raised by immunization of mice, and Western blotting confirmed that CsAK3 was expressed in adult-stage C. sinensis. Histochemical analysis showed that CsAK3 was localized to the subtegumental tissue of C. sinensis and was excreted into the bile duct of the host. When tested against sera from various parasite-infected patients by enzyme-linked immunosorbent assay, the recombinant CsAK3 elicited a specific response to C. sinensis-infected sera. The results suggest that CsAK3, either alone or in combination with other antigens, could be used for improving the clinical diagnosis of clonorchiasis.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2018 

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