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The metabolism of glucose by Haemonchus contortus, in vitro

Published online by Cambridge University Press:  06 April 2009

P. F. V. Ward
P. F. V. Ward
Affiliation:
Biochemistry Department, A.R.C. Institute of Animal Physiology, Babraham, Cambridge, CB2 4AT
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Extract

Adult Haemonchus contortus obtained from freshly killed sheep were incubated in a medium similar to Tyrode's saline containing known amounts of D-[U-14C]glucose. The worms appeared to remain in a healthy condition throughout the incubations which lasted for up to 6 h. All the radioactivity was recovered either within the worms or in the incubation vessel in the form of CO2, excretory products and unmetabolized glucose. An oxygen electrode in the incubation liquid showed that, because of their rapid oxygen consumption, the worms were under anaerobic conditions during all or nearly all of the incubation period, despite the presence of oxygen in the gas phase of the vessel. In terms both of quantity and radioactivity the main excretory products in solution were propan-1-ol, propionate and acetate. Smaller amounts of ethanol, lactate and succinate were excreted. Much radioactivity was associated with the expired CO2. At the end of the incubations glucose other than the initial radioactive glucose was detected indicating that the absorption of glucose by H. contortus is complex and needs further investigation. The present experiments suggest that the rate of absorption depends on the worm's need for nutrients. The results are compared with those found for other parasitic nematodes, particularly Ascaris lumbricoides, Heterakis gallinae and Trichuris vulpis. The importance of CO2 fixation in the utilization of energy from glucose by H. contortus is discussed.

Type
Research Article
Information
Parasitology , Volume 69 , Issue 2 , October 1974 , pp. 175 - 190
Copyright
Copyright © Cambridge University Press 1974

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References

REFERENCES

Allen, S. H. G., Kellermeyer, R. W., Stjernholm, R. L. & Wood, H. G. (1964). Purification and properties of enzymes involved in the propionic acid fermentation. Journal of Bacteriology 87, 171–87.CrossRefGoogle ScholarPubMed
Brand, T. von (1966). Biochemistry of Parasites, p. 92. New York, London: Academic Press.Google Scholar
Bueding, E., Kmetec, E., Swartzwelder, C., Abadie, S. & Saz, H. J. (1961). Biochemical effects of dithiazanine on the canine whipworm Trichuris vulpis. Biochemical Pharmacology 5, 311–22.CrossRefGoogle Scholar
Fairbairn, D. (1954). The metabolism of Heterakis gallinae. II. Carbon dioxide fixation. Experimental Parasitology 3, 52–63.CrossRefGoogle Scholar
Hall, T. C. & Cocking, E. C. (1965). High efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins. Biochemical Journal 96, 626–33.CrossRefGoogle ScholarPubMed
James, A. T. & Piper, E. A. (1963). A compact radiochemical gas chromatograph. Analytical Chemistry 35, 515–20.CrossRefGoogle Scholar
Jeffay, H. & Alvarez, J. (1961). Liquid scintillation counting of carbon-14. Analytical Chemistry 33, 612–5.CrossRefGoogle Scholar
Leaver, F. W., Wood, H. G. & Stjernholm, R. (1955). The fermentation of three carbon substrates by Clostridium propionicum and Propionibacterium. Journal of Bacteriology 70, 521–30.CrossRefGoogle ScholarPubMed
Prichard, R. K. (1970). In Annual Report, Division of Animal Health, p. 58. Commonwealth Scientific and Industrial Research Organization, Melbourne, Australia.Google Scholar
Ramsay, J. A. & Brown, R. H. J. (1955). Simplified apparatus and procedure for freezingpoint determinations upon small volumes of fluid. Journal of Scientific Instruments 32, 372–5.CrossRefGoogle Scholar
Rogers, W. P. (1949). On the relative importance of aerobic metabolism in small nematode parasites of the alimentary tract. Australian Journal of Scientific Research B 2, 157–74.Google Scholar
Saz, H. J. (1969). Carbohydrate and energy metabolism of nematodes and acanthocephala. In Chemical Zoology, vol. 3 (ed. Florkin, M. and Scheer, B. T.), pp. 329–60. New York, London: Academic Press.CrossRefGoogle Scholar
Saz, H. J. & Lescure, O. L. (1969). The function of phosphoenolpyruvate carboxykinase and malic enzyme in the anaerobic formation of succinate by Ascaris lumbricoides. Comparative Biochemistry and Physiology 30, 49–60.CrossRefGoogle ScholarPubMed
Thivend, P. (1974). Digestion of starch in the intestine of sheep. Proceedings of the Nutrition Society 33, 7A.Google ScholarPubMed
Ward, C. W., Schofield, P. J. & Johnstone, I. L. (1968). Carbon dioxide fixation in Haemonchus contortus larvae. Comparative Biochemistry and Physiology 26, 537–44.CrossRefGoogle ScholarPubMed
Ward, P. F. V. & Crompton, D. W. T. (1969). The alcoholic fermentation of glucose by Moniliformis dubius (Acanthocephala), in vitro. Proceedings of the Royal Society B 172, 65–88.Google ScholarPubMed
Ward, P. F. V. & Huskisson, N. S. (1972). The metabolism of fluoroacetate in lettuce. Biochemical Journal 130, 575–87.CrossRefGoogle Scholar