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Intraspecific and interspecific pairing of Haematoloechus medioplexus (Trematoda) and Echinostoma revolutum (Trematoda) adults in vitro, and observations on H. medioplexus lipophilic excretory–secretory products

Published online by Cambridge University Press:  06 April 2009

B. Fried  and
D. A. Leiby
B. Fried
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042
D. A. Leiby
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042
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Summary

Intraspecific pairing of Haematoloechus medioplexus and Echinostoma revolutum adults and interspecific pairing of H. medioplexus and E. revoltum adults were studied in vitro at 21 ± 1 °C and 30 ± 1 °C in Petri-dish cultures containing an agar substrate and a Locke's 1:1 solution overlay. Salinity and temperature tolerance tests showed that both species survived for 24 h in a Locke's 1:1 solution at 21 ± 1 °C and 30 ±1 °C. For behaviour studies, worms were placed 2 cm apart and then observed at various intervals from 5 min to 4 h. H. medioplexus and E. revolutum showed significant intraspecific pairing at 30±1 °C, whereas only H. medioplexus paired significantly at 21±1°C. Significant interspecific pairing of H. medioplexus and E. revolutum occurred at 21 ±1 °C and 30 ±1 °C. Thin-layer chromatographic analysis showed that H. medioplexus adults released mainly free sterol, along with traces of triacylglycerols and free fatty acids into a non-nutrient medium.

Type
Research Article
Information
Parasitology , Volume 84 , Issue 2 , April 1982 , pp. 375 - 380
Copyright
Copyright © Cambridge University Press 1982

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