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Culture of microsporidia from invertebrates in vertebrate cells

Published online by Cambridge University Press:  06 April 2009

Judith E. Smith ,
Rosalind J. Barker  and
P. F. Lai
Judith E. Smith
Affiliation:
Department of Pure and Applied Biology, Imperial College, LondonSW1 2AZ
Rosalind J. Barker
Affiliation:
Department of Pure and Applied Biology, Imperial College, LondonSW1 2AZ
P. F. Lai
Affiliation:
Department of Pure and Applied Biology, Imperial College, LondonSW1 2AZ
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Extract

Nosema algerae and N. eurytremae were successfully cultured in embryonic rat brain, Xenopus XTC-6 and Chang liver cells, and in embryonic rat brain and XTC-6 cells respectively. No parasites grew in cultures incubated at 38 °C but development took place in cells incubated at 34 °C and 27 °C. Increased levels of infections of the cultured cells were achieved by centrifugation of the spores on to the cells. The level of infection was also related to the type of medium used for hatching the spores, modifies NMRI giving better results than Leibowitz L15, and to the interval between adding the spores to the cell cultures and centrifugation.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 3 , December 1982 , pp. 427 - 435
Copyright
Copyright © Cambridge University Press 1982

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References

Beaudoin, R. L., Strome, C. P. A. & Clutter, W. G. (1974). Cultivation of avian malaria parasites in mammalian liver cells. Experimental Parasitology 36, 355–9.CrossRefGoogle ScholarPubMed
Canning, E. U. (1976). Report of work conducted in collaboration with G. C. Higby and B. M. Pilley. Proceedings of the 1st International Colloquium on Invertebrate Pathology, Kingston. p. 88.Google Scholar
Gupta, K. S. (1964). Cultivation of Nosema mesnili (Microsporidia) in vitro. Current Science, India 33, 407–8.Google Scholar
Ishihara, R. & Sohi, S. S. (1966). Infection of ovarian tissue culture of Bombyx mori by Nosema bombycis spores. Journal of Invertebrate Pathology 8, 538–40.Google Scholar
Kurtti, T. J. & Brooks, M. A. (1976). Propagation of Microsporidia in invertebrate cell culture. Proceedings of the 1st International Colloquium on Invertebrate Pathology, Kingston, p. 123.Google Scholar
Kurtti, T. J. & Brooks, M. A. (1977). The rate of development of a microsporidian in moth cell culture. Journal of Invertebrate Pathology 29, 126–32.CrossRefGoogle Scholar
Lai, P. F. & Canning, E. U. (1982). Some factors affecting spore replication of Nosema algerae (Microspore, Nosematidae) in Pieris brassicae (Lepidoptera). Journal of Invertebrate Pathology (in the Press).Google Scholar
Ohshima, K. (1937). On the function of the polar filament of Nosema bombycis. Parasitology 29, 220–4.Google Scholar
Pilley, B. M., Canning, E. U. & Hammond, J. C (1978). The use of microinjection procedure for large-scale production of the microsporidian Nosema eurytremae in Pieris brassicae. Journal of Invertebrate Pathology 32, 355–8.Google Scholar
Smith, J. E. & Sinden, R. E. (1980). A technique for the culture of Nosema algerae in primary cultures of rat brain. Journal of Protozoology 27, 59 A.Google Scholar
Sohi, S. S. & Wilson, G. G. (1976). Persistent infection of Malacosoma disstrae (Lepidoptera, Lasicompidae) cell cultures with Nosema (Glugea) disstriae (Microsporidia, Nosematidae). Canadian Journal of Zoology 54, 336–42.CrossRefGoogle Scholar
Streett, D. A., Ralph, D. & Hink, W. F. (1980). Replication of Nosema algerae in three insect cell lines. Journal of Protozoology 27, 113–17.Google Scholar
Trader, W. (1937). The hatching of spores of Nosema bombycis Nägeli and the partial development of the organism in tissue cultures. Journal of Parasitology 23, 226–7.Google Scholar
Undeen, A. H. (1975). Growth of Nosema algerae in pig kidney cell cultures. Journal of Protozoology 22, 107–9.Google Scholar
Undeen, A. H. & Alger, N. E. (1976). Nosema algerae: Infection of the white mouse by a mosquito parasite. Experimental Parasitology 40, 86–8.CrossRefGoogle ScholarPubMed