Skip to main content Accessibility help
×
Home
Hostname: page-component-cf9d5c678-m9wwp Total loading time: 0.256 Render date: 2021-08-03T05:34:15.276Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Article contents

Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi

Published online by Cambridge University Press:  01 December 1997

J. ARAYA
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil Unidad de Parasitologia, Universidad de Antofagasta, Av. Coloso s/n, Casilla 170, Antofagasta, Chile
M. I. CANO
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil
H. B. M. GOMES
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil
E. M. NOVAK
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil
J. M. REQUENA
Affiliation:
Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Canto Blanco 28049, Madrid, Spain
C. ALONSO
Affiliation:
Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Canto Blanco 28049, Madrid, Spain
M. J. LEVIN
Affiliation:
Ingebi, Obligado 2490, 1428 Buenos Aires, Argentina
P. GUEVARA
Affiliation:
Centro De Biologia Celular, UCV, Apdo 47525, Caracas, Venezuela
J. L. RAMIREZ
Affiliation:
Centro De Biologia Celular, UCV, Apdo 47525, Caracas, Venezuela
J. FRANCO DA SILVEIRA
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil

Abstract

We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. Sequences homologous to C6 are interspersed in the genome and can be mapped out on most chromosomal bands of different T. cruzi strains. The copy number of the C6 element is about 1000 per haploid genome. Given the species specificity and different genomic distribution of C6 homologous sequences among the T. cruzi strains the C6 element could be a useful probe for diagnosis and typing of parasites. C6 is a polymorphic marker with potential as a tool for physical mapping of the T. cruzi genome.

Type
Research Article
Copyright
1997 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)
14
Cited by

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *