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203Hg and other gamma-emitting radio-isotopes as labels for Dirofilaria immitis microfilariae

Published online by Cambridge University Press:  06 April 2009

F. W. Lengemann ,
R. B. Grieve ,
M. Chmielewicz  and
J. R. Georgi
F. W. Lengemann
Affiliation:
Department of Physiology and Preventive Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853
R. B. Grieve
Affiliation:
Department of Physiology and Preventive Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853
M. Chmielewicz
Affiliation:
Department of Physiology and Preventive Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853
J. R. Georgi
Affiliation:
Department of Physiology and Preventive Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853
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Summary

The in vitro uptake of gamma-emitting radionuclides by microfilariae of Dirofilaria immitis was investigated. Radionuclides tested were 133Ba, 207Bi, 82Br, 109Cd, 51Cr, 60Co, 59Fe, 203Hg, 125I, 54Mn, 32P, 125Sb, 75Se, and 65Zn. Only 207Bi, 59Fe, 203Hg, 54Mn, and 46Sc showed more than 2% of the available radioactivity to bind to the microfilariae. When tested for retention in vitro only 203Hg showed retention levels approaching 90%. Moreover, when dimethyl suiphoxide was incorporated into the medium at levels of 1% (v/v) the uptake of 203Hg could be increased by 3–5 times; no other radio-isotope tested responded in this manner. The uptake of 203Hg was directly related to temperature and time of incubation. Mercury, as mercuric chloride, was toxic to the microfilariae and respresents an impediment to the incorporation of high levels of 203Hg in microfilariae.

Type
Research Article
Information
Parasitology , Volume 92 , Issue 2 , April 1986 , pp. 451 - 461
Copyright
Copyright © Cambridge University Press 1986

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References

REFERENCES

Bolton, A. E. & Hunter, W. M. (1973). The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. The Biochemical Journal 133, 529–38.Google Scholar
Casarett, A. P. (1968). Radiation Biology, pp. 150–151, 255–256. Englewood Cliffs, New Jersey: Prentice Hall, Inc.Google Scholar
Christensen, N. O. (1977). A method for the in vivo labeling of Schistosolna mansoni and S. intercalatum cercariae with radioselenium. Zeitschrift für Parasitenkunde 54, 275–88.CrossRefGoogle ScholarPubMed
Dulbecco, R. & Vogt, M. (1954). Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99, 167–82.Google Scholar
Georgi, J. R. (1982). Schistosoina mansoni: quantification of skin penetration and early migration by differential external radioassay and autoradiography. Parasilology 84, 263–81.Google Scholar
Grieve, R. B., Mika-Grieve, M., Lok, J. B., Marchell, T. F. & Cupp, E. W. (1984). Recovery and viability of Dirofilaria immjtis microfilariae. Ada Tropica 41, 271–8.Google Scholar
Hunter, W. M. & Greenwood, F. C. (1962). Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194, 495–6.CrossRefGoogle ScholarPubMed
Morgan, J. R., Morton, H. J. & Parker, R. C. (1950). Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proceedings of the Society for Experimental Biology and Medicine 73, 1–8.Google Scholar
Knight, W. B., Laird, F., Ritchie, L. S., Pellegrino, J. & Chirobaga, J. (1968). Labelling of Biomphalaria glabrata and cercariae of Schistosoma mansoni with radioseleniunl. Experimental Parasitology 22, 309–15.Google Scholar
Smith, T. D. & Richards, P. (1975). A simple kit for the preparation of 99mTc-labeled red blood cells. Journal of Nuclear Medicine 19, 126–32.Google Scholar
Wood, D. C. & Woon, J. (1975). Pharmacologic and biochemical considerations of dimethyl sulfoxide. Annals of the New York Academy of Sciences 243, 7–19.CrossRefGoogle ScholarPubMed