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Identification of a new laccase gene and confirmation of genomic predictions by cDNA sequences of Trametes sp. I-62 laccase family

Published online by Cambridge University Press:  01 August 2003

Tania GONZÁLEZ ,
María del Carmen TERRÓN ,
Ernesto ZAPICO ,
Susana YAGÜE ,
Alejandro TÉLLEZ ,
Howard JUNCA  and
Aldo GONZÁLEZ
Tania GONZÁLEZ
Affiliation:
Centro de Investigaciones Biológicas, Velázquez 144, E-28006 Madrid, Spain. E-mail: aldo@cib.csic.es Present addresses: Instituto Cubano de Derivados de la Caña de Azúcar, Havana, Cuba;
María del Carmen TERRÓN
Affiliation:
Centro de Investigaciones Biológicas, Velázquez 144, E-28006 Madrid, Spain. E-mail: aldo@cib.csic.es
Ernesto ZAPICO
Affiliation:
Centro de Investigaciones Biológicas, Velázquez 144, E-28006 Madrid, Spain. E-mail: aldo@cib.csic.es Biotechnology Department, University of Hamburg, D-21073, Hamburg, Germany;
Susana YAGÜE
Affiliation:
Centro de Investigaciones Biológicas, Velázquez 144, E-28006 Madrid, Spain. E-mail: aldo@cib.csic.es
Alejandro TÉLLEZ
Affiliation:
Centro de Investigaciones Biológicas, Velázquez 144, E-28006 Madrid, Spain. E-mail: aldo@cib.csic.es Departmento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa, S. Rafael Atlixco n° 186, Col. Vicentina, C.O. 09340 México D.F., México;
Howard JUNCA
Affiliation:
Centro de Investigaciones Biológicas, Velázquez 144, E-28006 Madrid, Spain. E-mail: aldo@cib.csic.es Department of Environmental Microbiology, GBF-National Research Centre for Biotechnology, D-38124 Braunschweig, Germany.
Aldo GONZÁLEZ
Affiliation:
Centro de Investigaciones Biológicas, Velázquez 144, E-28006 Madrid, Spain. E-mail: aldo@cib.csic.es
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Abstract

The strain Trametes sp. I-62 (CECT 20197) is a white-rot fungus with great potential for biotechnological applications in the fields of industrial waste water decolorization and clean up. Three laccase genes: lcc1, lcc2 and lcc3 have been cloned and sequenced from this basidiomycete. In this work, the coding regions of the corresponding cDNAs have been synthesized, cloned, and sequenced. They are 1563, 1563 and 1575 bp in length, respectively. Former putative intron/exon structures from genomic DNA are fully confirmed by match analysis with our cDNA sequences. Using Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR–RFLP) analysis, an additional laccase cDNA was also identified, corresponding to a new gene, lcc1A, which displayed 99.6% identity with lcc1 at protein level. Such high similarity between lcc1 and lcc1A sequences, and the comparison with reports from other basidiomycete laccases, suggest that in this strain these two genes are allelic variants.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 6 , June 2003 , pp. 727 - 735
Copyright
© The British Mycological Society 2003

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