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Dynamic changes of rDNA condensation state during mitosis in filamentous fungi revealed by fluorescence in situ hybridisation

Published online by Cambridge University Press:  23 September 2003

Masatoki TAGA ,
Dai TSUCHIYA  and
Minoru MURATA
Masatoki TAGA
Affiliation:
Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan. E-mail: mtaga@cc.okayama-u.ac.jp
Dai TSUCHIYA
Affiliation:
Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan. E-mail: mtaga@cc.okayama-u.ac.jp
Minoru MURATA
Affiliation:
Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan.
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Abstract

We visualised rDNA of two ascomycetes, Cochliobolus heterostrophus and Haematonectria haematococca, by fluorescence in situ hybridisation (FISH) and analysed the condensation state of rDNA during mitosis. Both fungi showed a similar course of change in rDNA condensation corresponding to different mitotic stages. rDNA was decondensed in its entire length at interphase, and became increasingly condensed during prophase. The condensation reached a maximum at metaphase, remaining in that state through anaphase. Metaphase observation revealed the single distal location on a chromosome of rDNA in each fungus. This study provides the first visual evidence of the cyclic change of the condensation/decondensation state of rDNA during mitosis in filamentous fungi by FISH.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 9 , September 2003 , pp. 1012 - 1020
Copyright
© The British Mycological Society 2003

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