Earwigs (Insecta, Dermaptera) show astonishing diversity in penis morphology. In several families, males have a single penis (termed virga), whereas males of other families possess two functional virgae. Taxonomists have assumed that the two-virgae state is ancestral; however, ecological reasons why the ancestor acquired two virgae have not been explored. This study investigated in detail male and female genital structures, mating behaviour and insemination processes in the earwig Diplatys flavicollis (Diplatyidae). Diplatyidae are considered to be the most primitive family of earwigs. SEM and light-microscopy revealed that males of this species have two gonopores on each of two virgae, similar to those reported in other diplatyids (i.e. two double-barrelled penises), while females have four to six independent sperm-storage organs (spermathecae). Rapid fixation of mating pairs and insemination success of males from which one virga had been removed clearly revealed that only one virga was used for mating and was usually sufficient for inseminating multiple spermathecae. This finding rejects the one-to-one correspondence between male gonopores and female spermathecae. Based on allometric analysis of spermathecal variation, the possible significance of multiple spermathecae in relation to sperm-storage strategies of females is discussed. Compared to studies of male genital morphology, few studies have described spermathecal morphology. Based on compiled data of spermathecal and virgal morphology among earwigs, a parallel evolutionary trend between spermathecae and virgae from complicated (multiple) to simple (single) ones is suggested, and further investigation of the phylogeny, female genital morphology and insemination processes among earwigs is encouraged.

Specific procedures are described to measure the body dimensions of large African predators; these include measurements of body and tail length, head, canines, mane, limbs, paws and testes. Data on body mass and dimensions were collected from an immobilized sub-adult male African lion Panthera leo. He was 40 months old and weighed 190 kg. After being immobilized, data on body mass and dimensions were collected by a team comprising a scribe, two operators and assistants. The procedures of measuring the 43 variables on body dimensions were then repeated three times in quick succession, each session lasting about 10 min. The four sessions were handled as if a different individual was measured each time. Except for reports on body mass of lions, there is a paucity of comparable data in the literature for most of the variables presented in this paper. The small dispersion measures of the 43 variables suggest that the specific procedures to measure the body dimensions of a large African predator such as the lion have a high degree of repeatability and can be applied with confidence. The procedure has been used to obtain data on body dimensions of a large number of lions as well as dead specimens of black-backed jackal Canis mesomelas, caracal Caracal caracal and Cape fox Vulpes chama. The data form part of a database on body dimensions of large African predators created by ALPRU. The objective is to develop non-invasive techniques to determine whether wild predator populations might have been subjected to abnormal growth and development as a result of negative impacts on their habitat and thus food base.

Male secondary sexual traits may increase the risk of predation because mating signals make them conspicuous to predators and hamper evasive manoeuvres. Males of Xenotoca variata, a viviparous freshwater fish, show on their flanks bright and colourful spots (speckles), the number of which varies geographically. In this study, the association of this variation with the presence of the piscivorous snake Thamnophis melanogaster, which co-occurs with X. variata, was investigated. A test was also done to establish whether the snake distinguishes between male fish with contrasting numbers of speckles and if the perception of speckles is influenced by water turbidity. The amount of speckling and the prevalence of snakes in key localities was assessed by using one-way mirrors, and the effect of speckles on the predatory responsiveness of snakes was evaluated by presenting them with pairs of male fish in clear and in turbid water. In localities where snakes were infrequent there was a tendency for male fish to have many speckles. The snakes preferentially approached the males with more speckles than the males with fewer speckles. The direction of the preference did not change with the conditions of the water, but the magnitude was stronger in clear water than in turbid water. The snakes also approached first the males with more speckles. These findings indicate that predation risk by T. melanogaster may select against speckles and produce population differentiation.

Post-metamorphic growth patterns were analysed in the tropical caecilian amphibian Ichthyophis cf. kohtaoensis (Amphibia: Gymnophiona) experimentally in captivity over 6 years. Two questions were addressed. (1) Does this Ichthyophis display sexual dimorphism for size? (2) Do ichthyophiid caecilians express an indeterminate growth pattern? Total length did not differ between sexes in the first, second or third year after metamorphosis. In the fourth, fifth and sixth years, females were significantly larger than males. The index of sexual size dimorphism increased from zero in the first year after metamorphosis to 0.104 in the sixth year. Relative growth rates of total length were different for each sex. In the first two growth seasons males grew at higher rates, while from the third growth season females grew faster than males. In general, growth rates increased from the first to the second year after metamorphosis and decreased dramatically after both sexes became mature. As all Ichthyophis in this study grew constantly throughout the first 6 years after metamorphosis, indeterminate growth probably occurs in caecilians as reported for frogs and salamanders. Additionally, growth curves for both sexes of Ichthyophis cf. kohtaoensis were estimated based on the von Bertalanffy model.

For many burrowing species of mammals, the shape of the burrow may affect the success with which resources are acquired. Parameters used to characterize burrows have included: number of branches, mean segment length, turn angle, and linearity. However, the interactions among these parameters are unclear, making it desirable to find a single measure to characterize burrow geometry. For this reason, fractal dimension has been proposed as a measure of burrow geometry. However, the ways in which fractal dimension relates to other burrow metrics are not obvious. In this study, we examine correlations between fractal dimension and other measures of burrow geometry, using burrows of the pocket gopher Thomomys bottae. We also investigate whether burrows excavated by pocket gophers of different age, sex, and at two locations differing in food abundance have different fractal dimensions. We show that there are strong correlations between fractal dimension and other measures of burrow geometry, but that replacing fractal dimension with any single measure of burrow geometry discards important information about burrow structure. We conclude that fractal dimension offers a useful way of describing burrow geometry. No differences were found in burrow fractal dimension between pocket gophers of different ages or sex or in sites of different productivities, and we conclude that the factors that determine the pattern of burrowing in this species are invariant with respect to these parameters.

The mating system displayed by a species or population is a complex result of interactions among many factors, including social organization and resource distribution. Lekking is rare among mammals, and despite decades of research and theoretical debate, there is still little agreement concerning the socio-ecological conditions that give rise to this mating system. This study documents a lek-like mating system in a population of white-nosed coatis Nasua narica: the first such system observed in a carnivore species. Male coatis in Tikal National Park, Guatemala, aggregated around mobile bands of females during the mating season. The males displayed from the forest canopy above the bands, and females ascended to a male's perch when they were ready to mate. Males did not provide resources or parental care. Thus, the mating system, which differed from classic lekking only in the lack of an arena, can be best described as mobile lekking. Male coatis apparently resort to self-advertisement when the large size and large home ranges of female groups make defence of females or resources impractical.

The tiger quoll or spotted-tailed quoll Dasyurus maculatus, a nationally threatened species, is the largest surviving carnivorous marsupial on mainland Australia and the sole surviving member of the genus in south-eastern mainland Australia. Live-trapping and radio-tracking studies at three sites in south-eastern Australia found that tiger quolls were solitary, except when mating, and that male and female tiger quolls occupied very large home ranges, both during and after the breeding season. The mean size of the home range of male tiger quolls (minimum convex polygon (MCP) 1755.4 ha, kernel 3761.7 ha) was significantly larger than the mean size of the home range of females (MCP 495.9 ha, kernel 1113.0 ha). Adult female tiger quolls displayed intrasexual territoriality throughout the year, but they seemed to tolerate the presence of female offspring. Male tiger quolls were not territorial and their home ranges overlapped both with other males and with females throughout the year. Males were recorded regularly moving back and forth between a number of female territories during the breeding season. The spatial organization of the tiger quoll recorded in this study differs markedly from that of the eastern quoll Dasyurus viverrinus and the Tasmanian devil Sarcophilus harrisii, but is similar to that of the western quoll D. geoffroii, northern quoll D. hallucatus and brush-tailed phascogale Phascogale tapoatafa. The spatial organization and home-range size of the tiger quoll dictate that conservation of the species requires management across all land tenures at the landscape level.

Partial (DNA) sequences were examined for one nuclear (28S rRNA gene) and one mitochondrial (16S rRNA) locus for nine species of pomatiopsid snail (Gastropoda: Rissooidea: Pomatiopsidae) from south-east Asia and south-west China. Fresh field samples were collected for the following taxa: Delavaya dianchiensis (Triculinae: Triculini) from Dianchi lake, Yunnan Province, China; Neotricula aperta (Triculinae: Pachydrobiini) from north-east Thailand; Neotricula burchi from northern Thailand; Oncomelania hupensis robertsoni (Pomatiopsinae: Pomatiopsini) from south-west China; Tricula bambooensis (Triculinae: Triculini) from Dianchi lake; Tricula bollingi from northern Thailand; Tricula hortensis from Sichuan Province, China; Tricula ludongbini from Dianchi lake; and Tricula xiaolongmenensis from Dianchi lake. This work represents the first published DNA-sequence data for the Dianchi lake taxa and the first 28S sequence data for all nine taxa. All of these taxa with the exception of N. burchi and the Dianchi taxa transmit Schistosoma in nature; N. aperta and O. h. robertsoni transmit Schistosoma to humans. The data were used to estimate phylogenies using the maximum likelihood method, a Bayesian method, and the maximum parsimony method. The paper aims to examine the relationships between N.aperta, N. burchi and T. bollingi and the relationship between the Chinese taxa and the south-east Asian taxa; these relationships being important in evaluating certain historical biogeographical hypotheses. Good congruence was found between the phylogenies estimated by the three methods for both the 16S and 28S loci. However, poor congruence was found between the phylogenies based on the 16S and 28S data when the maximum likelihood and Bayesian methods were used. The lack of congruence is explained as a consequence of a rapid, recent, endemic radiation of the Yunnanese Tricula spp.; this hypothesis is consistent with current historical biogeographical models for the Triculinae. The paper puts forward dispersal hypotheses, based on palaeogeographical changes, as possible explanations for the phylogenetic relationships estimated here and for the current biogeography of these taxa.

Large-scale anthropogenic habitat degradation is taking place in the Negev Desert and such degradation is considered to be the primary cause of population decline in many bat species. A study of bat community structure in the central Negev Desert Highlands was undertaken to examine habitat associations, activity patterns and the distribution of bats in relation to landscape structure. During 1999 and 2000, mist-nets and bat detectors were used to capture bats and to monitor their presence in natural and artificial sites. In total, 12 species of insectivorous bats were found. Communities of insectivorous bats were divided into three guilds based on foraging space: open space foragers, background-cluttered space foragers, and highly cluttered space foragers. Bats of the background-cluttered space guild foraged over a variety of habitats while bats from the other guilds were more restricted with regard to their foraging spaces. In both years, season had a significant effect on bat activity. Despite there being significantly more bat activity in artificial sites than in natural sites, bat species richness was least in the artificial sites. For most species of desert-dwelling bats, areas that are typified by dense vegetation cover and have a perennial water source are of primary importance.

Many small carnivores include rodents in their diet. However, due to varying evolutionary strategies, carnivores differ in their metabolism and energy requirements. Hence, comparisons of diet between carnivores would be more meaningful if the body size and energetics of the predators are considered. The diet of three small carnivores (jungle cat Felis chaus; caracal Caracal caracal; golden jackal Canis aureus) from a semi-arid part of western India was studied through scat analysis, and the importance of rodents in their diet was estimated as a percentage of their daily energy requirement. Although percentage frequency in scats and biomass consumption showed rodents to be equally important to all three carnivores, energy calculations showed that rodents were more important as prey for the felids than the jackal. Up to 70% of the daily metabolizable energy in felids was obtained from rodents, as compared to 45% in the jackal. Rodents are generally viewed as pests, and their importance to the small carnivore community is overlooked. Change in land use over the decades in the arid/semi-arid tract of western India has led to several adverse as well as favourable modifications in rodent assemblages, which could influence the persistence of species like the caracal and jungle cat in this region, that largely depend on rodents for their survival.

The purpose of this study was to determine whether hibernating European hamsters Cricetus cricetus, L., 1758 stay in their burrows all winter or if they occasionally spent time above ground. If ‘time (spent) above ground’ (TAG) occurred regularly throughout hibernation, the second objective of this work was to categorize and quantify the patterns of TAG during winter. For this purpose, 11 European hamsters were individually housed in wire mesh cages that were buried in the ground of an outdoor enclosure. Each cage was filled with soil up to ground level allowing the animals to construct their own burrows within the cages. Antennae in the bottom and top of the cage were calibrated to receive signals only when the hamsters were in their burrows or above the ground, respectively. This method enabled continuous recordings of the animal's TAG and provided ecologically meaningful results as the animals were housed in a way that closely resembled their natural habitat. About half of the hamsters stayed continuously below ground. Of these hamsters, almost all showed a dense pattern of episodes of reduced body temperature separated by only brief periods of normothermia. In contrast, the other half of the animals moved either episodically or even regularly above ground. For all of these hamsters, TAG was significantly reduced as compared to normothermic values. One hamster did not show any hibernation but did have a significantly reduced normothermic body temperature and did not leave its burrow for periods of up to 3 weeks.

Tibetan argali Ovis ammon hodgsoni and blue sheep Pseudois nayaur have almost completely overlapping distributions encompassing most of the Tibetan plateau and its margins. Such a sympatric distribution of related species with similar ecological requirements implies that there is some degree of resource partitioning. This may be accomplished on the basis of habitat and/or diet separation. This study evaluated such ecological separation on the basis of physical habitat partitioning by these two sympatric ungulates in Hemis High Altitude National Park, Ladakh, India, in an area where the argali established a small new population in 1978. Such separation was tested for on the basis of expected difference between the species in their proximity to cliffs, associated with species-specific anti-predator behaviour. Tibetan argali selected habitats away from cliffs while blue sheep selected habitats close to cliffs. Blue sheep also selected steep slopes whereas argali selected gentle slopes. The two species did not differ in their use of habitats in terms of elevation. They did, however, differ in their use of plant communities; blue sheep selected sub-shrub and grass-dominated communities whilst argali selected forb-dominated communities. We suggest that the two species coexist in this site as a result of the differential use of habitat associated with their species-specific anti-predator strategies.

Acoustic monitoring and radio-tracking were used to study the nocturnal activity of adult female Indiana bats Myotis sodalis at a maternity site in Michigan, U.S.A. Pregnant bats did not use the day roost at night, but lactating females returned 2–4 times/night for 32±7 (SE) min/visit, presumably to feed their young. Both pregnant and lactating females night roosted as solitary individuals in trees within their foraging areas; night roosting occurred 0–6 times/night for 14±1 min each time. Bats foraged for most of the night, with the total duration of flight equalling 375±16 min/night. The bats used 13 different foraging areas that were located 0.5–4.2 km from the day roost. Bats did not fly over open fields but travelled along wooded corridors, even though such behaviour increased commuting distance by 55±11%. Current models of habitat suitability for this endangered species should be modified, taking into account the use of wooded commuting corridors and the large home range of these bats.

The seasonal activity of the common dormouse Muscardinus avellanarius was studied in three different habitat types of central Italy: a deciduous beech wood dominated by Fagus sylvatica (Cimini Mountains), and a deciduous oak woodland with Quercus cerris and Q. pubescens and an evergreen wood dominated by holm oak Q. ilex, both along the central Tyrrhenic coast. In each site, 50 nest-boxes were fixed to trees and were monitored monthly for 2 years (May 1998–April 2000). In the beech wood site, the species showed an annual biological cycle similar to that described for populations of central and northern Europe: dormancy in winter, from the first 10 days of November to the end of December; end of hibernation in the first half of April; reproduction between May and July. On the contrary, in the two coastal sites, nest-boxes were occupied from October to June, and the breeding period was in autumn, showing that a winter dormancy phase is absent, probably owing to the mild winter. On the other hand, no dormice or new nests were observed in nest-boxes in summer suggesting that a dormancy phase occurred in summer, probably owing to dry climatic conditions. Two patterns of biological cycle therefore emerged from the results: a temperate pattern in beech forest with winter dormancy and spring reproduction, and a Mediterranean pattern in coastal habitats with autumnal reproduction, winter activity and summer dormancy.

The African rodent genus Saccostomus is common and widespread in the savannas, scrubby areas and cultivated fields from south Ethiopia and Somalia through East Africa down to the Cape Province. Its systematics and taxonomy are however poorly known, with two species currently recognized, S. mearnsi and S. campestris, the former is typical of the Somali–Maasai domain and the latter is typical of Zambezian–South African. The karyotypic findings and the analysis of the mitochondrial gene cytochrome b for some populations from Somalia, Tanzania, Zambia and South Africa are presented showing that the diversity within the genus is much higher than previously reported and believed, and that S. mearnsi and S. campestris rather represent species complexes, still actively speciating. The times of cladogenetic events and the extent of chromosomal variability within the genus that may be coupled with speciation are discussed. Finally, the possible taxonomy is considered in the context of the wide karyotypic variation shown by each species, the extent and the boundaries of which are still largely unknown, limiting therefore their definitive taxonomic assignment.

American mink Mustela vison are an invasive species in many parts of the world (e.g. Europe, Iceland and South America) and in many of these areas they are a threat to the native wildlife. It is therefore important to establish a reliable and efficient method to estimate their relative abundance in order to manage their populations. In this study, the use of surveys of signs (scats and footprints) as a way to estimate the relative abundance of American mink was evaluated. Populations of mink in three areas of England were studied to evaluate the impact of three factors (surveyor, marking places and month) on the probability of deposition and detection of signs. Among these factors only month had a significant effect. In the second part of the study, sign surveys were compared with live trapping and it was found that the proportion of sections with mink signs was only loosely correlated to the abundance of mink as estimated from live trapping. In conclusion, this study suggests that, provided that surveyors are trained, that there is a sufficient number of marking places in each section, and that the surveys are carried out in comparable seasons, sign surveys are an appropriate way to monitor the relative abundance of mink. Sign surveys are better than trapping when it is necessary to monitor mink populations over large areas because they are less time consuming, require less manpower and are cheaper.

The claw geometry of birds can be used to predict their mode of life. Previous studies, however, have not considered how bird size might affect these predictions. Thus, in the present study, the geometric scaling relationships of bird claws are examined for a variety of extant birds with different modes of life: predatory, climbing, perching or ground-dwelling. Measurements of hind-limb claw radius (i.e. claw size) and claw angle (i.e. claw ‘hookedness’) of the third digit claw were made on 120 species of bird ranging from 0.0057 kg to 44 kg in body mass. Claw radius was found to be proportional to (body mass)0.34 across all species. Claw angle was found to increase with body mass for predatory and climbing birds (i.e. bigger birds have relatively more hooked claws), and decrease with body mass for ground-dwelling birds (i.e. bigger birds have relatively less hooked or flatter claws). No significant relationship was found between claw angle and body mass for perching birds. Mode of life could not be predicted with any certainty using measurements of either claw radius or claw angle, suggesting difficulty in assigning fossil species such as Archaeopteryx to a specific locomotor category. As claw design should enable the claw to withstand the forces placed upon it, further work is needed to establish the stresses experienced by the claws of different types of bird.

This study was an investigation of the diet and of prey selection by a population of African wild dogs Lycaon pictus in the Savé Valley Conservancy (SVC) in the south-east lowveld of Zimbabwe. The diet of the dogs, based on the number of kills for each species located, consisted almost entirely of impala Aepyceras melampus (74%) and kudu Tragelaphus strepsiceros (22%), the two most abundant medium-sized antelope in the area. The level of selection for different prey classes killed by the wild dogs was investigated and it was found that the wild dogs: (1) exhibited strong selection for a number of prey classes on a seasonal basis; (2) selected kudu throughout the year but showed little selection between the other prey species; (3) selected the young of both impala and kudu and selected males at times of the rut. Bone marrow analysis showed that prey killed by the wild dogs on SVC were in poorer condition than unselectively culled individuals suggesting the selection of the weaker individuals in the prey populations.

This paper reports on efforts to trap jaguars Panthera onca on camera in the dry forests of the Kaa-Iya del Gran Chaco National Park in Bolivia. Ad hoc camera trapping provided certain information on jaguar presence and habits, but was limited in application. Activity patterns showed that jaguars are active all day, particularly at one of three sites, with peaks in the morning and evening the more common pattern. Minimum observed home range was variable, with males (up to 65 km2) occupying more area than females (up to 29 km2). The authors adapted systematic methodologies first developed to survey tigers in India, based on individually distinctive pelage patterns in tigers and jaguars. Abundance is estimated using capture–recapture statistical analysis, and a sample area defined based on the maximum distance that individual jaguars move during the sample period. The methodology has proved successful for jaguars in dry Chaco forest, population densities of 1/30–45 km2 and 1/20 km2 are estimated in the two most extensive landscape systems of Kaa-Iya. The entire 34 400 km2 protected area is estimated to sustain a population of over 1000 adult and juvenile jaguars, the largest single population of jaguar reported anywhere, and a viable population for long-term jaguar conservation.