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In vitro mineralization on chitosan using solutions with excess of calcium and phosphate ions

Published online by Cambridge University Press:  01 December 2005

Marisa Masumi Beppu ,
Marco Antonio Torres ,
Cassiano Gomes Aimoli ,
Gilberto Alessandre Soares Goulart  and
Cesar Costapinto Santana
Marisa Masumi Beppu*
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
Marco Antonio Torres
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
Cassiano Gomes Aimoli
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
Gilberto Alessandre Soares Goulart
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
Cesar Costapinto Santana
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
*
a)Address all correspondence to this author. e-mail: beppu@feq.unicamp.br
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Abstract

Pseudo-simulated body fluids (SBFs) were used in in vitro experiments to promote chitosan porous membrane calcification. Common SBFs, which had concentrations of phosphate or calcium ions doubled, were so named because they do not replicate, by rigor, a genuine body fluid ion concentration. The objective of using such calcification fluids was to study the influence of phosphate and calcium excess in solution on mineralization deposit characteristics. SEM-EDS analyses showed that morphology and composition of deposits varies depending on which ion (phosphate or calcium) is in excess; x-ray diffractograms show that deposits are poorly crystalline (like biological apatites) but still show better crystallinity in deposits generated from P-rich SBF. This result, added to previous ones [such as those reported by Beppu and Santana Mater. Res.5, 47 (2002)] where a difference in the interconnectivity of the inorganic and organic (matrix) phases was stressed, suggests different deposition processes for each situation.

Keywords

BiomedicalBiomimeticCoating
Type
Articles
Information
Journal of Materials Research , Volume 20 , Issue 12 , December 2005 , pp. 3303 - 3311
Copyright
Copyright © Materials Research Society 2005

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References

REFERENCES

1.Beppu, M.M. and Santana, C.C.: Influence of calcification solution on in vitro chitosan mineralization. Mater. Res. 5, 47 (2002).CrossRefGoogle Scholar
2.Pathak, Y., Shoen, F.J. and Levy, R.J.: Pathologic calcification of biomaterials, in Biomaterials Science: An Introduction to Materials in Medicine, edited by Ratner, B.D., Hoffman, A.S., and Schoen, F.J. (Academic Press, San Diego, CA, 1996), p. 272.Google Scholar
3.Sandford, P.A.: Chitosan: commercial uses and potential applications, in Chitin and Chitosan, edited by G. Skjaek-Braek. (Elsevier Applied Science, New York, 1989), p. 51.Google Scholar
4.Beppu, M.M. and Santana, C.C.: Influence of acetylation on in vitro chitosan membrane biomineralization. Key Eng. Mater. 31, 192 (2001).Google Scholar
5.Kokubo, T., Ito, S., Huang, Z.T., Hayashi, T., Sakka, S., Kitsugi, T. and Yamamuro, T.: Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. J. Biomed. Mater. Res. 24, 331 (1990).Google Scholar
6.Davies, J.E.: Mechanisms of endosseous integration. 1st COLAOB Conference, Belo Horizonte, Brazil, 1998.Google Scholar
7.Mucalo, M.R., Toriyama, M., Y, Y. Yokogawa, Suzuki, T., Kawamoto, Y., Nagata, F. and Nishizawa, K.: Growth of calcium-phosphate on ion-exchange resins pre-saturated with calcium or hydrogenophosphate ions—an SEM/EDX and XPS study. J. Mater. Sci.-Mater. Med. 6, 409 (1995).Google Scholar
8.Ren, J. and Jiang, C.: Transport phenomena of chitosan membrane in pervaporation of water–ethanol mixture. Sep. Sci. Technol. 33, 517 (1998).Google Scholar
9.Golomb, G. and Wagner, D.: Development of a new in vitro model for studying implantable polyurethane calcification. Biomaterials 12, 397 (1991).CrossRefGoogle ScholarPubMed
10.Saito, H., Tabeta, R. and Ogawa, K.: High-resolution solid-state 13C NMR study of chitosan and its salts with acids: conformational characterization of polymorphs conformational-dependent 13C chemical shifts. Macromolecules 20, 2424 (1987).Google Scholar
11.Ogawa, K., Hirano, S., Miyanishi, T., Yui, T. and Watanabe, T.: A new polymorph of chitosan. Macromolecules 17, 973 (1984).Google Scholar
12.Kim, H.M., Kishimoto, K., Miyaji, F., Kokubo, T., Yao, T., Suetsugu, Y., Tanaka, J. and Nakamura, T.: Composition and structure of the apatite formed on PET substrates in SBF modified with various ionic activity products. J. Biomed. Mater. Res. 46, 228 (1999).Google Scholar
13.Kim, H.M., Kishimoto, K., Miyaji, F., Kokubo, T., Yao, T., Suetsugu, Y., Tanaka, J. and Nakamura, T.: Composition and structure of apatite formed on organic polymer in simulated body with a high content of carbonate ion. J. Mater. Sci.-Mater. Med. 11, 421 (2000).Google Scholar
14.Oyane, A., Onuma, K., Ito, A., Kim, H.M., Kokubo, T. and Nakamura, T.: Formation and growth of clusters in conventional and new kinds of simulated body fluids. J. Biomed. Mater. Res. 64A, 339 (2003).Google Scholar
15.Bayraktar, D. and Tas, A.C.: Chemical preparation of carbonated calcium hydroxyapatite powders at 37 °C in urea-containing synthetic body fluids. J. Eur. Ceram. Soc. 19, 2573 (1999).CrossRefGoogle Scholar
16.Calvert, P. and Rieke, P.: Biomimetic mineralization in and on polymers. Chem. Mater. 8, 1715 (1996).CrossRefGoogle Scholar
17.Keller, A., Hikosaka, M., Rastogi, S., Toda, A., Barham, P.J. and Goldbeck-Wood, G.: An approach to the formation and growth for new phases with application to polymer crystallization: Effect of finite size, metastability, and Ostwald’s rule of stages. J. Mater. Sci. 29, 2579 (1994).CrossRefGoogle Scholar
18.Liu, X. and Ding, C.: Morphology of apatite formed on surface of wollastonite coating soaked in simulate body fluid. Mater. Lett. 57, 652 (2002).Google Scholar
19.Landi, E., Tampieri, A., Celotti, G., Langenati, R., Sandri, M. and Sprio, S.: Nucleation of biomimetic apatite in synthetic body fluids: Dense and porous scaffold development. Biomaterials 26, 2835 (2005).Google Scholar
20.Hölland, W., Rheinberger, V. and Frank, M.: Mechanisms of nucleation and controlled crystallization of needle-like apatite in glass-ceramics of the SiO2–Al2O3–K2O–CaO–P2O5 system. J. Non-Cryst. Solids 253, 170 (1999).Google Scholar
21.Brown, P.W.: Phase relationships in the ternary system CaO–P2O5–H2O at 25 °C. J. Am. Ceram. Soc. 75, 17 (1992).Google Scholar
22.Aoba, T.: Solubility properties for human tooth mineral and pathogenesis of dental caries. Oral Dis. 10, 249 (2004).Google Scholar
23.Christoffersen, J., Dohrup, J. and Christoffersen, M.: Kinetics of growth and dissolution of calcium hydroxyapatite in suspension with variable calcium to phosphate ratio. J. Cryst. Growth 186, 275 (1998).Google Scholar
24.Tung, M.S.: Calcium Phosphate in Biological and Industrial System (Kluwer Academic Publishers, Hingham, U.K., 1998), p. 1.Google Scholar
25.Handbook of Chemistry and Physics, 2003, edited by Lide, David R. (CRC Press, Boca Raton, FL), p. 4.Google Scholar
26.Weiner, S. and Traub, W.: Bone structure: from angstroms to microns. FASEB J. 6, 879 (1992).CrossRefGoogle ScholarPubMed
27.Karlsson, K.H.: Bone implants—a challenge to materials science. Ann. Chir. Gynaecol. 88, 226 (1999).Google Scholar
28.Takadama, H., Kim, H.M., Kokubo, T. and Nakamura, T.: Mechanism of biomineralization of apatite on a sodium silicate glass: TEM-EDEX study in vitro. Chem. Mater. 13, 1108 (2001).CrossRefGoogle Scholar
29.Rahzi, M., Desbrières, J., Tolaimate, A., Rinaudo, M., Vottero, P., Alagui, A. and Meray, M. El: Influence of the nature of the metal ions on the complexation with chitosan. Application to the treatment of liquid waste. Eur. Polym. J. 38, 1523 (2002).Google Scholar
30.Tianwei, T., Xiaojing, H. and Weixia, D.: Adsorption behaviour of metal ions imprinted chitosan resin. J. Chem. Technol. Biotechnol. 76, 191 (2001).Google Scholar
31.Mann, S.: Biomineralization and biomimetic materials chemistry. J. Mater. Chem. 5, 935 (1995).Google Scholar
32.Nielsen, A.E.: Electrolyte crystal growth mechanisms. J. Cryst. Growth 67, 289 (1984).Google Scholar
33.Nielsen, A.E. and Toft, J.M.J.: Electrolyte crystal growth kinetics. J. Cryst. Growth 67, 278 (1984).CrossRefGoogle Scholar
34.Li, P., Nakanishi, K., Kokubo, T. and de Groot, K.: Introduction and morphology of hydroxyapatite, precipitated from metastable simulated body fluids on sol-gel prepared silica. Biomaterials 14, 963 (1993).Google Scholar