Two experiments were carried out to study the efficiency of energy utilization by Suffolk × Blackface lambs. In the first experiment, a digestibility trial, the lambs were fed on either a roughage (chopped dried grass), concentrate (whole barley, ground barley and fishmeal), or mixed (roughage and concentrate) diet. The dry matter (DM) and organic matter digestibility coefficients of the mixed diet were slightly, but not significantly, lower than the ‘expected’ values calculated by summation of the separately determined digestibility coefficients of the component feeds. Similarly, the metabolizable energy (ME) value was 1·6% lower than the ‘expected’ value.

In the second experiment, lambs were offered 52·5 kg roughage DM (ME 9·96 MJ/kg DM) and 52·5 kg concentrate DM (ME 11·59 MJ/kg DM) as:(i) a mixed diet (M); (ii) a progressive change from roughage to concentrate (R–C); (iii) a progressive change from concentrate to roughage (C–R); or 105 kg roughage or concentrate DM as either (iv) a roughage diet (R), or (v) a concentrate diet (C). Lambs on treatments R–C, C–R and R and C combined took a similar time to consume their allocation of food and achieved a similar final empty body weight (EBW) and empty body composition. In addition, the total energy retention (ER) of lambs on these treatments was similar to that predicted by a feeding standards model for lambs of a similar liveweight offered diets of the same ME content. Lambs on treatment M consumed their allocation of food faster than those on all other treatments and achieved a slightly, but not significantly, higher final EBW, which contained a higher proportion of fat. The ER was higher than predicted. The higher overall efficiency of lambs on treatment M was attributed to higher daily intakes and differences in the ratio of protein: fat deposited and maintained by lambs on each treatment. The adoption of separate efficiency constants for both deposition and maintenance of protein and fat in current feeding standards would result in a more accurate prediction of animal performance.

Ammonia volatilization from stored slurry or surface-applied slurry in the field is strongly affected by pH. Thus a simple, iterative model was developed to predict pH changes in slurry. Concentrations of NH4+/NH3, CO2/HCO3-/CO32- and volatile fatty acids were input data to the model. The model was validated by titrating 17 slurry samples collected from four cattle farms, seven pig farms and three biogas plants. Predictions of pH agreed well for 14 slurries with titration data in the pH interval from 4 to 10. Simulations indicated that microbial degradation of VFA to methane and carbon dioxide resulted in a pH increase if the carbon dioxide produced was lost to the atmosphere. There was little change in pH if the produced carbon dioxide dissolved in the slurry.

The effects of severe food restriction and of protein supplementation on the apparent digestibility of a forage were studied on 12 wethers in 1992 at INRA, Theix. Ruminal digestion of the forage cell walls was studied in a second experiment carried out on four ewes fitted with rumen and duodenal cannulas, receiving the same diets as the wethers. The animals were fed on two different hay-based diets: hay only (58 or 25 g/kg BW0·75 per day) or hay supplemented with fish meal (48 or 20 g hay with 6·5 and 3·0 g fish meal respectively per kg BW0·75). The effects of fish meal supplementation on apparent digestibility of the hay cell walls were small, + 1·8 and + 2·3 units for neutral detergent fibre (NDF) and acid detergent fibre (ADF) respectively. In contrast, NDF and ADF digestibility was respectively 4·0 and 4·7 units higher at low than at high intake. Rumen fluid volume decreased by 2·4 1 with decreasing intake, while water concentration in rumen content increased slightly from 89·8 to 91·8%. The mean retention time of the hay particles in the foreguts increased concomitantly from 39·5 to 55·1 h. This was achieved by a higher retention time to a similar extent in both slow and fast compartments. The calculated in situ degradability of NDF and ADF increased respectively by 12·1 and 13·1 units with decreasing intake. This reflected the lower fractional outflow rate recorded at low intake. If a fractional outflow rate value of 4·1%/h (as measured in high intake diets) was applied to all dietary treatments, NDF and ADF in situ degradability would be greater at low than at high intake (31·7 v. 26·6% for NDF; 27·9 v. 22·1 % for ADF), indicating a higher microbial activity in restricted diets. Interactions between intake and fish meal supply were detected for in situ degradation parameters, indicating principally a reduction of the cell wall undegraded fraction in supplemented diets when intake declined. Ruminal fermentation was modified by the dietary treatments, the acetate: propionate ratio being higher at low intake. Fish meal increased concentrations of ammonia and of isoacids in rumen liquid. The relationship between apparent digestibility and in situ degradability of plant particles is discussed.

The feeding behaviour of indigenous goats grazing on natural vegetation in a semi-arid region of Zimbabwe was studied over five seasons covering a period of one year (March 1989 to March 1990). The goats were randomly allocated to two groups, A and B, which grazed for 8 h (08.00–16.00 h) and 5 h (11.00–16.00 h) daily, respectively. These grazing times were based on the current practices of smallholder farmers. The botanical and chemical composition of the diet was determined by analysing samples collected from oesophageal fistulated goats. Daily feed intake was estimated by the total faecal collection method. Feed selection varied markedly with season and was similar in both groups. From January to March (wet season) grass formed 55–70% of the forage consumed, while from May to early November (dry period) browse, herbs and fruits formed 77–91% of the diet. Dietary crude protein levels ranged from 128 to 157 g/kg over the seasons. Neutral detergent fibre content ranged from 590 to 790 g/kg, and was highest in wet months and lowest in dry months. Feed intake ranged from 33 to 60 g/kg BW0·75 or 16–28 g/kg of body weight. It appeared that a feeding duration of 5 h per day was adequate for maintenance but insufficient for optimum production.

The physiological consequences of selection for weaning weight were investigated using 14-month-old castrated male Merino lambs from lines selected for (W +) and against (W – ) weight at weaning for over 10 generations. The selection experiment commenced in 1953 and was conducted at Trangie, NSW, Australia. Lambs from these lines now differ by 42% in weight at weaning and maturity, and have a proportionately similar composition of protein, fat and ash in their bodies at similar stages of maturity. In the whole body, there were no apparent between-strain differences in protein synthesis, but W + lambs had lower (P < 0·05) rates of phenylalanine oxidation than W – lambs. In hind limb muscle, protein metabolism of W + lambs was more responsive to food intake than in W – lambs. In particular, the rate of change of protein degradation differed (P < 0·05) between the lines in response to food intake per unit liveweight with protein degradation decreasing with increased feed intake in W + lambs. W + lambs had lower (P < 0·05) rates of blood flow to, and used less (P < 0·05) oxygen per kg hind limb muscle than W— lambs. Endocrine differences between the lines included increased (P < 0·05) plasma insulin-like growth factor-I (IGF-1) concentration in W + compared to W— lambs and, from regression analysis, an indication that there was a higher (P < 0·01) response in hind limb protein gain to insulin in the W + lambs.

These results suggest that selection for weaning weight has altered the dynamics of protein metabolism in lambs, principally through alterations in protein breakdown in muscle. Moreover, they suggest that there are associated genetic differences in endocrine control of protein metabolism which involve insulin and possibly IGF-I.

The effects of synchronizing dietary energy and nitrogen supply in diets with a similar carbohydrate composition on microbial protein synthesis and rumen fermentation were examined in sheep. Two diets were formulated to be either synchronous (diet S) or asynchronous (diet A) for the hourly release of nitrogen (N) and energy to the rumen. Diet S contained (g/kg) 425 g wheat straw, 400 g winter barley, 150 grapeseed meal and 25 g minerals/vitamins and diet A contained 505 g wheat straw, 458.5 g winter barley, 11·5 g urea and 25 g minerals/vitamins. Both diets were fed at the rate of 1 kg/day in four equal portions, to four cannulated sheep, in two periods in a change-over design. Rumen ammonia concentrations followed the predicted hourly trend in N degradation with a peak 1 h after feeding of 10 mM for diet S and 16 mM for diet A before falling within 3 h of feeding to 4 ITIM in animals fed either diet. Rumen volatile fatty acid (VFA) concentrations followed the cyclical trend predicted by stoichiometric equations, whilst rumen VFA ratios were more stable than predicted in animals fed either diet. The observed content of rumen degradable protein and organic matter truly degraded in the rumen was similar for both diets. The increase in total CHO digested in the rumen observed with diet A (427 g/kg DM) compared with diet S (364 g/kg DM) can be attributed to the greater content of starch in the asynchronous diet, which had a high degradability. The efficiency of microbial protein synthesis (g N/kg OM truly degraded in the rumen) was 11–20% greater in animals fed the synchronous diet (S) than the asynchronous diet (A). It is concluded that microbial N production was more efficient when dietary energy and N supply were synchronized.

Cumulative feed and water intakes and body weights of 30 growing ewe lambs of three different breeds were recorded weekly for 34 weeks between October 1990 and June 1991 at the Experimental Farm of the University of Pretoria. The results were analysed to obtain water efficiencies on both an absolute and metabolic weight gain basis. On an absolute basis, Mutton Merino sheep consumed 145% and Dorper 109% more water than Blackhead Persian sheep. On a metabolic weight gain basis, the corresponding differences were 92 and 66%, respectively. Mutton Merino and Dorper lambs also consumed 69 and 41% more water per kg feed intake than the Blackhead Persians, respectively. Weekly water consumption increased twice as much per 1 °C increase in ambient temperature in the Dorper and Mutton Merino lambs as in the Blackhead Persians. These results suggest that sheep of the Blackhead Persian, and probably also other indigenous breeds, balance their water requirements at a more economical level and would be a more appropriate choice than those breeds currently being managed in sustainable sheep production systems in areas where water availability is limited.

Sheep fitted with a ruminal and a duodenal cannula were used to study the effect of dry matter intake and the physical form of the diet on salivary P secretion and faecal endogenous P loss. At constant low P intake, changing the form of the diet had no effect on either parameter and while increasing dry matter intake did produce small changes in the partitioning of P excretion between urine and faeces it had no effect on overall endogenous P loss (faecal endogenous P + urine P) which was largely determined by P intake.