Greater cultivation of the underutilised Gac fruit, Momordica cochinchinensis, by poorly resourced householders and farmers would potentially improve livelihoods, and, on a larger scale, meet the increasing demand for Gac as a health product. Cultivation methods need to be developed to suit small- and large-scale production and must consider the unpredictable ratio of male to female plants grown from seed, and slow growth induced by cool temperatures. In this study, we examined the responses of Gac to propagation and protected cropping techniques to identify potential methods for increasing production. Plants germinated from seed in seed-raising mix under warm and humid conditions were grown hydroponically to maturity in a climate-controlled greenhouse during a temperate winter, producing fruits that were harvested ripe, from 44 weeks after sowing. Cuttings taken from female plants were dipped in indole-3-butyric rooting hormone powder or gel, or were left untreated, and then placed in rock wool, potting mix, water or closed media sachet. All treatment combinations, with the exception of the untreated potting mix, permitted the development of healthy plants in a second greenhouse crop. Growing plants from seed, then vegetatively increasing the number of productive female plants by cuttings is a means to increase Gac production with limited resources. Gac production using greenhouse technology, as described here for the first time, is relevant to other temperate regions. The finding that larger fruits have a higher percentage of edible aril than smaller fruits provides a new area of investigation towards enhancing production.

High temperature stress at flowering can adversely affect rice yield, largely due to failure of fertilization. Oxidative damage can be a major reason inducing spikelet sterility in rice. In the present study, the effect of high temperatures on antioxidant metabolism in rice spikelets was characterised using nine different genotypes. Exposure to different temperatures at flowering stage revealed significant differences among various antioxidant enzymes in spikelets, both quantitatively and qualitatively. Spikelets of susceptible genotypes withstood temperature stress of up to 35 °C, those of moderately tolerant between 35 °C and 38 °C and those of tolerant genotypes up to 40 °C. Presence or absence, and changes in the isozyme intensities were consistent with alterations in their activities. Superoxide dismutase (SOD) isozymes II and III were present after exposure at 30 °C and 35 °C, while SOD I appeared above 40°C. Intensities of catalase isozymes I and III and the only isozyme of ascorbate peroxidase altered, while the only isozyme of guaical peroxidase and two (III and IV) of the four isozymes of catechol peroxidase disappeared after high temperature exposure of 45 °C. Thus, this work provides an evidence of the role of antioxidant metabolism in spikelets under high temperature stress conditions. Hence, changes in antioxidant isozymes in rice spikelets can be used as a biomarker for characterizing high temperature stress tolerance in rice spikelets.

This experiment was conducted to study the long-term effect of soil management treatments on crop yields, sustainability yield indices (SYI) and soil fertility in rainfed semi-arid tropical Alfisol at Hayathnagar Research Farm, Hyderabad, India, during the period 1995 to 2009. The experiment was conducted in a split–split plot design with conventional tillage (CT) and minimum tillage (MT) as main factors, surface application of sorghum stover @ 2 t ha−1 (SS), fresh Gliricidia loppings @ 2 t ha−1 (GL) and ‘no’ residue (NR) as sub-factors and levels of N viz. 0 (N0), 30 (N30), 60 (N60) and 90 (N90) kg N ha−1 as sub–sub factors in a castor–sorghum two-year rotation. On an average, CT maintained 30.4 and 57.0% higher grain yields of sorghum and castor, respectively, over MT. Between two residues, GL performed well in both the crops. The highest yields of sorghum (1425 kg ha−1) and castor (876 kg ha−1) were recorded at 90 kg N ha−1. CT maintained higher SYI of 0.44 compared to MT (0.38) and higher agronomic efficiency (AE) of 13.5 and 6.76 kg grain kg−1 N for sorghum and castor crop, respectively. Use of crop residue as mulch had an advantage in increasing the yield of both the crops with increase in rainfall under CT even without N application (control), probably by making the soil more receptive to water infiltration, better moisture storage and by reducing the evaporative losses. Using response functions, the optimum fertilizer N requirement was also computed for a given set of tillage and residue combinations. The revised optimum fertilizer N doses for sorghum and castor varied from 45 to 56 kg ha−1 and 46 to 74 kg ha−1, respectively, under different tillage and residue combinations and could be recommended depending upon the soil management practices.

Data from three cocoa (Theobroma cacao) clonal selection trials are used to investigate the genetic and environmental components of variation in yield and the percentage of total pods affected by black pod disease (Phytophtora pod rot). Simulations based on these estimated components of variation are then used to discuss the best choice in future of numbers of clones, replicates and years of harvest to maximise selection advances in the traits measured. The three main conclusions are the need to increase the number of clones at the expense of the number of replicates of each clone, the diminishing returns from additional years of harvesting and the importance of widening the genetic base of the clones chosen to be tested.

Cyanobacteria represent promising organic inputs in rice–wheat cropping system, as they contribute towards accretion of N and C, besides secreting growth-promoting substances which influence plant productivity and soil fertility. The present study focused towards using a combinatorial approach for evaluating field-level colonization of cyanobacteria in soil and their effect on soil microbiological and plant parameters, employing agronomic and molecular tools. A consortium of cyanobacterial strains (BF1, Anabaena sp., BF2, Nostoc sp., BF3, Nostoc sp. and BF4, Anabaena sp.) was employed in different three-and four-member combinations along with 75% N + Full dose of P and K fertilizers. A significant enhancement in microbial activity and plant growth/yields and savings of 25% N in the wheat–rice cropping sequence were recorded, especially in treatments involving 75% N + Full dose of PK+BF1+BF2+BF4 and T5, i.e. 75% N + Full dose of PK+BF1+BF2+BF3. Such treatments were significantly higher or statistically at par with fertilizer controls – 75% N + Full dose of PK fertilizers. The use of DNA-based markers further helped to establish the colonization of the inoculated cyanobacteria, especially BF2 and BF3 strains. Our study clearly illustrated the establishment of inoculated cyanobacterial strains and their role in enhancing the crop productivity and soil health of the rice–wheat cropping system.

It is generally accepted that the two forms of Passiflora edulis, the golden and the purple, originated on the edges of tropical rainforests in Brazil. Extensive hybridisation has since taken place between these two forms and their hybrids. The passion fruit (a vine) is now grown throughout the tropics and subtropics. A limited amount of basic, fundamental research has been published on the water relations of passion fruit. Leaf production and expansion are both sensitive to water deficits, while water stress reduces leaf and floral bud initiation. A single axillary flower bud forms at each leaf node of new growth along with a tendril. Flower bud development and fruit set are less sensitive to water stress than leaf initiation. Heavy rain during pollination prevents fertilization. Unevenness in crop distribution during the year is possibly linked to water stress and temperature variation. Potential evapotranspiration rates in Brazil varied between 3.5 mm d−1 and 5.8 mm d−1. The value for the crop coefficient increases from about 0.6 during apical vegetative growth up to about 1.25 during flowering and fruiting. Water productivities still need to be determined. Micro-sprinklers and drip are the most effective ways of applying irrigation water with precision to passion fruit. Opportunities exist for international cooperation in research projects of mutual interest on passion fruit water relations.

The positive role of plant-derived smoke on seed germination and post-germination processes is well documented. The present study examined if plant-derived smoke with various methods of application influence the agronomic performance of a traditional cereal crop, tef [Eragrostis tef (Zucc.) Trotter]. Comparisons were made in potted tef plants, which germinated from seeds treated with smoke-water (1:500 dilution), Karrikinolide1 (KAR1, 10−8 M) solutions and seeds pretreated with cool aerosol-smoke for 10 min (rinsed and unrinsed afterwards). The smoke-related treatments modified a number of physiological, morphological and agronomic features of Eragrostis tef. Compared with the control, KAR1 and aerosol-smoke treatment of the seeds significantly improved plant height. All the smoke-related treatments significantly promoted stem-thickness whereas number of tillers and grain and dry biomass responded positively to aerosol-smoke and smoke-water treatments. These findings indicate that the plant-derived smoke treatment has a great potential to improve grain and dry biomass yields of tef. Moreover, due to its positive role in improving stem-thickness, smoke treatment may assist in combating lodging problems in cereals such as rice, wheat and barley, provided they are responsive to smoke treatments.

The macadamia nut is the only commercial food crop indigenous to Australia. It originated along the fringes of rainforests, only becoming an important internationally traded crop in the late 20th century. Australia is now the main centre of production, following a lead set by Hawai'i. The crop is also important in Central America and southern Africa. The majority of recent research reported in the literature on the physiology and water relations of macadamia has been undertaken in Australia. Macadamia is a tall evergreen tree in which vegetative growth occurs in a series of flushes. Mild water stress suppresses growth, but compensatory growth occurs on re-watering. Flowers form on hardened wood within the leaf canopy. Floral initiation occurs in late autumn, with flowering in the following spring. Flowering is profuse but only 5–10% of the flowers set fruit and less than 1% of the flowers reach maturity as fruits due largely to premature abscission. High temperatures (>30 °C) can contribute to the shedding of fruit. It takes about 12 months from floral initiation to the completion of harvest. Macadamia root systems are described as relatively shallow and spreading. The taproot can extend to depths of >1.2 m, with a dense matt of fibrous roots in the top 0.4 m. Proteoid rootlets are also present. Stomata are only found on the abaxial surface of the leaf. Macadamia has several attributes that help to explain its apparent tolerance to periodic dry conditions as found in its native habitat. These include leaves with xeromorphic adaptations, stomatal closure without concurrent changes in leaf water potential and an efficient water transport system. There has only been one serious attempt to measure the actual water use of macadamia. Most irrigation experiments have been poorly planned and/or the outcomes were inconclusive. Nut yields are cyclical and highly variable, making it difficult to establish cause and effect. It is not yet possible to say with evidence where and when irrigation is worthwhile.

The results of research on the water relations and irrigation need of avocado are collated and reviewed in an attempt to link fundamental studies on crop physiology to irrigation practices. Background information is given on the centre of origin (Mexico and Central America) and the three distinct ecological areas where avocados are grown commercially: (1) Cool, semi-arid climates with winter-dominant rainfall (e.g. Southern California, Chile, Israel); (2) Humid, subtropical climates with summer-dominant rainfall (e.g. eastern Australia, Mexico, South Africa); and (3) Tropical or semi-tropical climates also with summer-dominant rainfall (e.g. Brazil, Florida and Indonesia). Most of the research reported has been done in Australia, California, Israel and South Africa. There are three ecological races that are given varietal status within the species: Persea americana var. drymifolia (Mexican race), P. americana var. guatemalensis (Guatemalan race) and P. americana var. americana (Antillean, West Indian or Lowland race). Interracial crossing has taken place. This paper summarises the effects of water deficits on the development processes of the crop and then reviews plant–water relations, crop water requirements, water productivity and irrigation systems. Shoot growth in mature trees is synchronised into flushes. Flower initiation occurs in the autumn, with flowering in late winter and spring. Flowers form on the ends of the branches. A large heavily flowering tree may have over a million flowers, but only produce 200–300 fruits. Fruit load adjustment occurs by shedding during the first three to four weeks after fruit set and again in early summer. Water deficits during critical stages of fruit ontogeny have been linked to fruit disorders such as ring-neck. Reproductive growth is very resistant to water stress (compared with vegetative growth). Avocado is conventionally considered to be shallow rooted, although roots extend to depths greater than 1.5 m. The majority of feeder roots are found in the top 0.60 m of soil and root extension can continue throughout the year. Leaves develop a waxy cuticle on both surfaces, which is interrupted by stomata on the abaxial surface (350–510 mm−2), many of which are blocked by wax. Stomata are also present on the sepals and petals at low densities (and on young fruit). During flowering, the canopy surface area available for water loss is considerably increased. Stomatal closure is an early indicator of water stress, which together with associated changes in leaf anatomy, restricts CO2 diffusion. There have only been a few attempts to measure the actual water use of avocado trees. In Mediterranean-type climates, peak rates of water use (in summer) appear to be between 3 and 5 mm d−1. For mature trees, the crop coefficient (Kc) is usually within the range 0.4–0.6. The best estimate of water productivity is between 1 and 2 kg fruit m−3. Soil flooding and the resultant reduction in oxygen level can damage roots even in the absence of root rot. Avocado is particularly sensitive to salinity, notably that caused by chloride ions. Rootstocks vary in their sensitivity. Both drip and under-tree microsprinklers have been/are successfully used to irrigate avocado trees. Mulching of young trees is a recommended water conservation measure and has other benefits. A large proportion of the research reviewed has been published in the ‘grey’ literature as conference papers and annual reports. Sometimes, this is at the expense of reporting the science on which the recommendations are based in peer-reviewed papers. The pressures on irrigators to improve water productivity are considered.

The effects of leaves cut during fibre harvesting of Attalea funifera under contrasting irradiance availability were evaluated, studying defoliation-induced changes in photosynthetic and growth characteristics and fibre production in adult individuals of A. funifera growing under full sun and forest understorey. Fibre harvesting was performed with or without (control plants) leaf removal twice in a 12-month interval. Maximum measured values of net photosynthesis (A), stomatal conductance and transpiration were higher in full sun defoliated palms than in non-defoliated palms in the same environment or in understorey palms of the two treatments. Non-defoliated palms from the two environments emitted more leaves than defoliated palms in the same evaluation period. The increment in the leaf-level rate of carbon assimilation following defoliation did not lead to high production of fibre, suggesting that the photosynthetic compensation for leaf removal was not effective, at least during the period of evaluation. The results indicate that leaf removal during harvesting did not represent an advantage for fibre production and may even lead to decreases in leaf and fibre production.

Date palm is a crop suited to hot, arid regions. It originated in Mesopotamia and the centres of production are in West Asia and North Africa. Despite its regional and international importance, and its dependence on irrigation or a shallow water table for survival, relatively little research has been published on the water relations and irrigation need of date palm. Following early work in California, the majority of the recent research reported in the literature has been conducted in Saudi Arabia and Tunisia. The date palm has a terminal crown of 100–120 leaves. At the base of each leaf is an axillary bud, most of which develop in the winter as flower buds. It takes 150–200 days from pollination to fruit maturity. Stomata occur on both leaf surfaces. Different techniques have been used to measure the water use of date palm, including micrometeorological and sap flow methods. In Syria, mean actual evapotranspiration rates varied between 0.5 mm d−1 (winter) and 3.5 mm d−1 (summer), in Saudi Arabia from 2–3 to 8–11 mm d−1 depending on location and in Jordan from 2 to 8–10 mm d−1 respectively. In Tunisia, there was some (limited) evidence of stomatal control of transpiration rates (seasonal range 0.5 to 3.5 mm d−1) when temperatures exceeded 32 °C. Experimentally determined values of the crop coefficient were inconsistent, varying from 0.6–0.7 to 1.18. In the vicinity of an oasis, advection may increase potential water use substantially above that of a reference crop. In the absence of a shallow water table, roots can extract water from soil depths greater than 2 m. There is little published information on the water productivity of date palm. A target benchmark figure is probably about 1.3-kg fresh fruit m−3 of irrigation water applied. Under controlled conditions, phenotypes differed in their responses to water stress. The date palm is traditionally considered to be relatively salt-tolerant, with a threshold electrical conductivity value for the saturated soil extract of 4.0 dS m−1, but recent evidence from Israel suggests that this view may be mistaken. Since early times, flood irrigation has been used to irrigate date palm and it is still probably the most common method in many countries. Since the 1980s, farmers have been encouraged by governments to use localised irrigation methods (e.g. micro-sprinklers, drip and bubbler) as a means of saving water. Since water is a scarce resource in the West Asia and North Africa region, research should focus on developing ways to improve the water productivity of this high value crop.

The results of research on the water relations and irrigation needs of olive are collated and summarised in an attempt to link fundamental studies on crop physiology to irrigation practices. Background information on the ecology of the olive (it is native to the coastal areas of the eastern Mediterranean) and on crop development processes are presented, followed by reviews of the influence of water stress on gas exchange (stomatal conductance, photosynthesis and transpiration), crop water requirements, water productivities and irrigation systems. The olive has many attributes that help to protect it against drought, including the morphology of the leaf, and the capacity to conserve water under conditions of high evaporative demand through stomatal closure, osmotic regulation and resistance to cavitation. The concept of ‘deficit irrigation’ has been the subject of much research. Although vegetative growth is restricted, there is no convincing evidence that ‘sustained deficit irrigation’ or ‘regulated deficit irrigation’ or ‘partial root zone drying’ offer any advantages over conventional practices. Water productivities are very variable and difficult to reconcile due, in part, to biennial bearing, tree-to-tree variability and differences in tree population densities. Similarly, no clear consensus has emerged on how best to exploit the sensitivity of trunk expansion to water availability in irrigation scheduling. As production methods for this historical crop are intensified (high-density hedgerows, irrigated and mechanized orchards), so will the need to understand the role that water plays in the production processes become ever more critical.

This paper explores changes in the organization of seed supply in China over the last decade by means of a multi-level institutional analysis. At the landscape level, the implications for China of the regulation of plant genetic resources through various international treaties and conventions are reviewed in the light of the evolution of the global seed industry. At the regime level, the transition in the Chinese context to market-based seed supply and the development of commercial and public seed sectors are examined. The study then analyses trends in seed supply at the niche level, with reference to participatory maize (Zea mays L.) breeding in three provinces in southwest China where high rural poverty persists. This work offers radical novelty in variety development and seed provision on behalf of smallholder farmers. However, a series of technical, organizational and market ‘mismatches’ are demonstrated within the existing seed regime. The participatory work emphasizes breeding for diverse cultivars adapted to specific ecosystems but these are prevented from reaching commercial markets by existing varietal testing procedures. Participatory breeding has potential to address farmers’ varietal needs as agriculture modernises and to support the public function of research institutes, but within mainstream intellectual property regimes the public value of participatory breeding cannot be accommodated adequately. Yet, when coupled to institutional innovations for recognising intellectual property and sharing benefit among all those who contribute, participatory breeding may initiate a powerful dynamics for change within seed regimes and a sui generis seed system suited to the Chinese context.

Regression models for the prediction of grain yields of non-irrigated winter wheat in a semi-arid Mediterranean environment were developed based on drought variability. Twenty-five years (1980–2004) of climate data and yield data from four soils (sandy loam, clay, clay loam and sandy clay loam soil) in northern Greece were used for this purpose. Two variables were selected as explanatory variables of the models: (a) the monthly precipitation versus the monthly reference evapotranspiration ratio (P/ETo), which describes the monthly drought and consequently the water deficit conditions during the wheat-growing season and (b) the mean observed yield (y) of each soil, which indirectly describes the intrinsic fertility of the soils. A resampling technique using subsets of the data (bootstrapping) was applied to estimate the coefficients of the models, to assess the uncertainty of the selected explanatory variables and to validate the models. The models showed adequate predictive ability of wheat yields, defining the time and intensity of drought effects. The most crucial period for winter wheat was found to be primarily the vegetative-reproductive stage period between late winter and mid-spring (i.e. February to April). Soil clay content was found to be the most representative parameter in describing most of the physico-chemical parameters and properties of the soils and consequently the mean yield, indicating that yield is non-linearly correlated with most soil properties. With the proposed models, yield gap (YG) predictions between two growing seasons of the selected soils presented 84% accuracy in all years in the identification of the correct signal (+ or −) of yield increase or decrease, respectively, and adequate performance in the prediction of the mean YG.

Changes in seed viability over 12 months of low-input storage conditions were monitored on five diverse seed species grown in the tropics: amaranth (Amaranthus cruentus), lablab bean (Lablab purpureus), moringa (Moringa oleifera), pumpkin (Cucurbita moschata) and tomato (Solanum lycopersicum). Because the costs of maintaining low-temperature storage can be prohibitively expensive in developing countries, this study explored alternatives to low-temperature storage at the Educational Concerns for Hunger Organization (ECHO) Asia Impact Center Seed Bank in Northern Thailand. Specifically, this research compared the effects of vacuum sealing and refrigeration on stored seed viability in both laboratory and field settings. While seed species was an influential factor in determining seed longevity, the relative importance of vacuum sealing and refrigeration differed for the dependent variables of seed moisture content, germination rate, mean time to 50% germination and field emergence. Although the combination of vacuum sealing and refrigeration was most effective at conserving seed quality as measured by each of these variables, the storage of seeds in vacuum-sealed packages at ambient temperatures was more effective than unsealed but refrigerated packets at conserving low moisture content and high germination and field emergence rates across species. This suggests that for resource-constrained seed banks in the tropics, vacuum sealing with or without refrigeration may represent a viable alternative to other expensive and energy-intensive storage techniques.

Salicylic acid (SA) can induce multiple stress tolerance in plants. This study investigated the relationship between SA and antioxidant enzyme activities in maize seedlings under chilling stress. Changes of endogenous SA, antioxidant enzyme activities and malondialdehyde (MDA) concentrations were assessed in two different chilling-tolerant maize inbred lines (Huang C and Mo17) under chilling stress. The results showed that both endogenous free and bound salicylic acid contents increased in roots and leaves of both lines. MDA concentrations also increased significantly in roots and leaves of both lines after chilling stress. In addition, in Huang C, chilling stress increased the activities of four antioxidant enzymes, ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR) and peroxidase, while in Mo17, only CAT and APX increased. Furthermore, a regression analysis was conducted between SA and MDA concentrations or antioxidant enzyme activities under chilling stress. The results indicated that MDA concentrations were positively correlated with total SA contents in roots (r = 0.9776, p = 0.0224) and bound SA in leaves (r = 0.9974, p = 0.0458), respectively. Total SA contents had positive correlations with APX activities both in roots (r = 0.9993, p = 0.002) and leaves (r = 0.9630, p = 0.037) and GR in leaves (r = 0.9298, p = 0.0221). Together, these results suggested that chilling stress improved the biosynthesis of endogenous SA, and lipid peroxidation and antioxidant enzyme activities could be indicated by endogenous SA contents of maize seedlings under chilling stress. Furthermore, increased activities of antioxidant enzymes, especially in roots, may contribute to the chilling tolerance of maize seedlings.

Farm households in the Andean region of South America face serious livelihood challenges, including a poor natural resource base and declining agricultural yields. Conservation agriculture has been identified as a potential solution to environmental degradation and the associated poverty and food insecurity in the region. This study analyses the potential economic impact of conservation agriculture in two sub-watersheds in central Ecuador utilizing a linear programming model and data from experiments in farmer fields. The model found that specific cover crops, crop rotations and reduced tillage designed to reduce soil erosion and increase soil organic matter can lead to increased incomes for farm households in a time period of as short as two years. It appears that conservation agriculture practices have the potential to improve the livelihoods of the rural poor in Ecuador because conservation agriculture activities entered the revenue-maximizing model solution for both sub-watersheds.

Using computer simulation model as a management tool requires model calibration and validation against field data. A three-year (2008–2009 to 2010–2011) field study was conducted at the Postgraduate Agricultural Research Station of the University of Agriculture, Faisalabad, Pakistan, to simulate wheat grain yield production as a function of urea fertilizer applications using Crop Environment REsource Synthesis (CERES)-Wheat model. The model was calibrated using yield data for treatment of urea fertilizer application at the rate of 247 kg-urea ha−1 during growing season 2009–2010 and was validated against independent data sets of yield of two years (2008–2009 and 2010–2011) for a wide variety of treatments ranging from no urea application to 247 kg-urea ha−1 application. The model simulations were found to be acceptable for calibration as well as validation period, as the model evaluation indicators showed a mean difference of 8.9%, ranging from 0.05 to 15.38%, root mean square error of 356 having its range from 242 to 471 kg ha−1, against all observed grain yield data. The scenario simulations showed maximum grain yield of 4100 kg ha−1 for 350 kg-urea ha−1 in 2008–2009; 4600 kg ha−1 for 300 kg-urea ha−1 in 2009–2010 and 5200 kg ha−1 for 340 kg-urea ha−1 in 2010–2011. Any further increase in urea application resulted in decline of grain yield function. These results show that model has the ability to simulate effects of urea fertilizer applications on wheat yield; however, the simulated maximum grain yield data need field-based verification.