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Transgenic plants

Published online by Cambridge University Press:  13 July 2009

Abstract

We can now genetically engineer many of the world's major crop plants. Generally, transformation strategies based on the soil bacterium Agrobacterium tumefaciens are used to generate transgenics, although success in cereal and legume transformation has been achieved by the introduction of DNA by particle bombardment. Plants have now been engineered to be tolerant of herbicides, produce fruit storable for prolonged periods, be resistant to insect predation and fungal pathogens, as well as have changed biochemical characteristics. Plant transformation can itself be used as a means of isolating genes from plants by gene tagging and this may provide a means of isolating plant genes of agronomic value. These advances, coupled with the recent demonstration of the ability of plants to produce novel pharmaceuticals and biodegradable plastics, have the potential to revolutionize agricultural practice, as well as to increase our knowledge of plant growth and development.

Type
Research Article
Copyright
Copyright © Academia Europaea 1996

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References

REFERENCES

1.Walden, R. (1988) Plant Genetic Transformation. Wiley, New York.Google Scholar
2.Baron, C. and Zambryski, P. (1995) Notes from the underground: highlights from plant—microbe interactions. TIBTECH 13, 56362.CrossRefGoogle Scholar
3.White, F. F. (1993) Vectors for gene transfer in higher plants. In Transgenic Plants Vol. 1 (Kung, D. and Wu, R., Eds) pp. 1548. Academic Press.CrossRefGoogle Scholar
4.Bechtold, N., Ellis, J. and Pelletier, G. (1993) In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C. R. Acad. Sci. Paris, Life Sciences 316, 11941199.Google Scholar
5.Hiei, Y., Ohte, S., Komari, T. and Kumashiro, T. (1994) Efficient transformation of rice mediated by gro bacterium and sequence analysis of the boundaries of T-DNA. Plant J. 6, 271282.CrossRefGoogle Scholar
6.Negruitiu, , et al. (1987) Hybrid genes in the analysis of transformation conditions. Plant Mol. Biol. 363–373.CrossRefGoogle Scholar
7.Christou, P. (1994) Genetic engineering of crop legumes and cereals: current status and recent advances. Agro Food Industry Hi-Tech. 5, 1727.Google Scholar
8.Wilson, T. M. A. and Davies, J. W. (1992) Genetic Engineering with Plant Viruses. CRC Press.Google Scholar
9.Kumagai, M. H. et al. (1993) Rapid, high level expression of biologically active alpha trichosanthin transfected plants by a viral vector. Proc. Nat. Acad. Sci. U.S.A. 90, 427430.CrossRefGoogle ScholarPubMed
10.Hamamoto, H. et al. (1993) A new tobacco mosaic virus vector and its use for the synthetic production of angiotensin—I—converting enzyme inhibitor in transgenic tobacco and tomato. Bio/Technology 11, 930932.Google Scholar
11.Reichel, C. et al. (1996) Enhanced green fluorence by the expression of an Aquequorea victoria GP mutant in mono- and dicotyledonous plant cells. Proc. Nat. Acad. Sci. U.S.A. submitted.CrossRefGoogle Scholar
12.Bourque, E. J. (1995) Antisense strategies for genetic manipulation in plants. Plant Science 105, 125149.CrossRefGoogle Scholar
13.Muller-Rober, B. T. (1992) Inhibition of ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage genes. EMBO 11, 12291238.CrossRefGoogle Scholar
14.Stitt, M. and Sonnewald, U. (1995) Regulation of metabolism in transgenic plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 46, 341368.CrossRefGoogle Scholar
15.Noling, J. W. and Becker, J. O. (1994) The challenge of research and extension to define and implement alternatives to methyl-bromide. J. Nematol. 26, 573586.Google ScholarPubMed
16.Urwin, P. E. et al. (1995) Engineered oryzacystin-I in transgenic hairy roots confers resistance Globodera pallida. Plant J. 8, 121131.CrossRefGoogle ScholarPubMed
17.Sijmons, P. C. et al. (1994) Parasitic strategies of root nematodes and associated host cell response. Ann. Rev. Phytopath. 32, 235259.CrossRefGoogle Scholar
18.Strittmatter, G. et al. (1995) Inhibition of fungal development in plants by engineering controlled cell death. Bio/Tech. 13, 10851089.CrossRefGoogle Scholar
19.Strittmatter, G. and Wegener, D. (1993) Genetic engineering of disease and pest resistance in plant present state of the art. Z. Naturforsch. 48c, 673688.CrossRefGoogle Scholar
20.Jach, G. et al. (1995) Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. Plant J. 8, 97109.CrossRefGoogle ScholarPubMed
21.Feitelson, J. S., et al. (1992) Bacillus thuringiensis: Insects and Beyond. Bio/Tech. 10, 271275.Google Scholar
22.Fitchen, J. F. and Beachy, R. N. (1993) Genetically engineered protection against viruses in transgenic plants. Ann. Rev. Microbiol. 47, 739763.CrossRefGoogle ScholarPubMed
23.Gressels, J. (1993) Plant Breeding Rev. 11, 155198.Google Scholar
24.Kramer, M. G. and Redenbaugh, K. (1994) Commercialisation of a tomato with an antisen polygalacturonidase gene: The FLAVR SAVR™ tomato story. Euphytica 79, 293297.CrossRefGoogle Scholar
25.Klee, H. et al. (1991) Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. Plant Cell 3, 11871193.Google ScholarPubMed
26.Altenbach, S. et al. (1990) Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine rich protein in transgenic plants. Plant Mol. Biol. 13, 513522.CrossRefGoogle Scholar
27.Shaul, O. and Galilli, G. (1992) Increased lysine synthesis in tobacco plants that express high levels of bacterial dihydropicolinate synthase in their chloroplasts. Plant J. 2, 203209.CrossRefGoogle Scholar
28.Shaul, O. and Galilli, G. (1992) Threonine overproduction in transgenic tobacco plants expressing mutant desensitised aspartate kinase of Escherichia coli. Plant Physiol. 100, 11571163.CrossRefGoogle ScholarPubMed
29.Shaul, O. and Galilli, G. (1993) Concerted regulation of lysine and threonine synthesis in tobacco plants expressing feed back insensitive aspartate kinase and dihydrodipicoline synthase. Plant Mol. Biol. 23, 759768.CrossRefGoogle Scholar
30.Tavladoraki, E. et al. (1993) Transgenic plants expressing a functional single-chain Fv antibody and specifically protected from virus attck. Nature 366, 469472.CrossRefGoogle Scholar
31.Ma, K. et al. (1995) Generation and assembly of secretory antibodies in plants. Science 268, 716719.CrossRefGoogle ScholarPubMed
32.Haq, T. A. et al. (1995) Oral immunization with a recombinant bacterial antigen produced transgenic plants. Science 268, 714716.CrossRefGoogle ScholarPubMed
33.Topfer, R. et al. (1995) Modification of plant lipid biosynthesis. Science 268, 681686.CrossRefGoogle Scholar
34.Hitz, W. D. et al. (1995) Reducing polyunsaturation in oils of transgenic canola and soybean. In Plant Lipid Metabolism. Kader, J-C. and Mazliak, P. (eds) Kluwer, Dordrecht, The Netherlands, pp. 506508.CrossRefGoogle Scholar
35.Stark, D. M. (1992) Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphorylase. Science 285, 287292.CrossRefGoogle Scholar
36.Muller-Rober, B. et al. (1992) Inhibition of the ADP-glucose pyrophosphorylase in transgenic potato leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes. EMBO J. 4, 12291238.CrossRefGoogle Scholar
37.Visser, R. G. F. et al. (1991) Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol. Gen. Genet. 225, 289296.CrossRefGoogle ScholarPubMed
38.van der Meer, I. M. et al. (1994) Fructan as a new carbohydrate sink in transgenic potato plants. Plant Cell 6, 561570.CrossRefGoogle ScholarPubMed
39.Nawrath, C., Poirier, Y. and Somerville, C. (1995) Plant polymers for biodegradable plastics: cellulose starch and polyhydroxyalkanoates. Molecular Breeding 1, 105122.CrossRefGoogle Scholar
40.Walden, R., Hayashi, H. and Schell, J. (1991) T-DNA as a gene tag. Plant J. 1, 281288.Google Scholar
41.Hayashi, H. et al. (1992) Activation of a plant gene by T-DNA tagging: auxin-independent growth vitro. Science 258, 13501353.CrossRefGoogle ScholarPubMed
42.Fritze, K. et al. (1995) T-DNA tagging of genes influencing polyamine metabolism: isolation mutant plant lines and rescue of DNA promoting growth in the presence of polyamine biosynthetic inhibitor. The Plant J. 7, 261272.CrossRefGoogle Scholar
43.United States Department of Agriculture (USDA); Animal and Plant Health Inspection Service. Internet http://www.aphis.usda.gov/bbep/bp.Google Scholar

Further reading

Chrispeels, M. J. and Sadava, D. E. (1994) Plants, Genes and Agriculture. Jones and Bartlett Publishers.Google Scholar
Kung, S. D. and Wu, R. (1992) Transgenic Plants (Vols 1 and 2). Academic Press.Google Scholar
Walden, R. (1988) Genetic Engineering in Plants. Wiley Scientific Publishers.Google Scholar
Tudge, C. (1988) Food Crops of the Future. Blackwell Scientific Press.Google Scholar
Marx, J. L. (Ed) (1989) A Revolution in Biotechnobgy. Cambridge University Press.Google Scholar