Hostname: page-component-848d4c4894-89wxm Total loading time: 0 Render date: 2024-07-08T03:42:10.148Z Has data issue: false hasContentIssue false

Anti-inflammatory properties of brilliant blue G on chronic unpredictable mild stress-induced changes in rat hippocampus

Published online by Cambridge University Press:  23 March 2020

F. Aricioglu
Affiliation:
Marmara University School of Pharmacy, Department of Pharmacology and Psychopharmacology Research Unit, Istanbul, Turkey
T. Bastaskin
Affiliation:
Marmara University School of Pharmacy, Department of Pharmacology and Psychopharmacology Research Unit, Istanbul, Turkey
C. Kandemir
Affiliation:
Marmara University School of Medicine, Department of Histology Embriology, Istanbul, Turkey
S. Sirvanci
Affiliation:
Marmara University School of Medicine, Department of Histology Embriology, Istanbul, Turkey
C. Ozkartal
Affiliation:
Marmara University School of Pharmacy, Department of Pharmacology and Psychopharmacology Research Unit, Istanbul, Turkey
T. Utkan
Affiliation:
Kocaeli University School of Medicine, Department of Pharmacology, Kocaeli, Turkey

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Objective

Purinergic 2X7 receptor (P2X7R) activation has recently been considered to be involved in depression at least partially by triggering microglial activation. The aim of the present study was to examine whether the chronic administration of brilliant blue G (BBG), a highly selective P2X7R antagonist, has antidepressant-like effects and microglial (Iba-1) immunoreactivity in chronic unpredictable mild stress (CUMS) model in rats.

Methods

Male Wistar Albino rats (290–360 g) were divided into groups such as control (saline), CUMS, CUMS + Imipramine (20 mg/kg; i.p.), CUMS + BBG25 (25 mg/kg; i.p.), CUMS + BBG50 (50 mg/kg; i.p.) groups (n = 10–12 in each). In CUMS model, various stressors were applied for 40 days. On day 20, the treatment of BBG was started for 20 days. At the end, sucrose preference and forced swimming tests were performed. Then brains were removed with paraformaldehyde perfusion for Iba-1 immunohistochemical analysis in hippocampus. One-way analysis of variance and Tukey's test were used for statistical analysis.

Results

The time of immobility in forced swim test was significantly reduced while sucrose preference was increased in Imipramine and CUMS + BBG50 groups compared to control and CUMS groups, respectively. In immunohistochemical experiments, Iba-1 was overexpressed in CUMS group and BBG significantly reduced the overexpression of Iba-1.

Conclusion

Our results suggest that chronic administration of BBG has an antidepressant-like activity supporting the notion of P2X7 receptors involvement in depression by modulating microglial activation.

This research was supported by grant from Marmara University, Scientific Research Projects – SAG-C-YLP-110915-0416 and SAG-E-120613-0233.

Disclosure of interest

The authors have not supplied their declaration of competing interest.

Type
e-Poster Walk: Psychopharmacology and pharmacoeconomics and psychoneuroimmunology
Copyright
Copyright © European Psychiatric Association 2017
Submit a response

Comments

No Comments have been published for this article.