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Synergistic Cooperation Between T and B Lymphocytes from Old Mice in the Production of Auto-Anti-Idiotypic Antibody Regulation in Adult Irradiated Hosts

Published online by Cambridge University Press:  29 November 2010

Myron R. Szewczuk
Myron R. Szewczuk
Affiliation:
Queen's University, Kingston
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Abstract

The effect of age on the ability of B lymphocytes and thymus cells from donors of various ages to be capable of producing an anti-idiotype-blocked, hapten-augmentable PFC was studied by adoptive cell transfer techniques. Lethally irradiated mice were reconstituted with syngeneic B lymphocytes and thymus cells from donors of various ages. Recipients were immunized with trinitrophenylated bovine gamma globulin (TNP-BGG) one or seven days after cell transfer. Splenic IgG anti-TNP plaque-forming cell (PFC) responses were assayed in the absence and presence of hapten for anti-idiotype (Id)-blocked, hapten-augmentable PFC, 14 days after immunization. It was found that the B lymphocyte population from 2 month old donors together with thymus cells from donors of various ages (2 to 19 months) were incapable of reconstituting mice to produce anti-Id-blocked, hapten-augmentable PFC. Similar results were obtained when mice were reconstituted with thymus cells from 2-month-old donors together with B cells from donors of various ages (2 to 14 months). In contrast, mice reconstituted with B cells plus thymus cells from the same 8-month or older donors produced a significantly high percentage of anti-Id-block, hapten augmentable PFC. Mice reconstituted with B cells from 8 months or older donors plus thymus cells from donors of various ages (8 to 19) months also produced a significantly high percentage of hapten-augmentable PFC. Experiments with B cells and thymus cells from 2-or 8-month old donors parked in lethally irradiated 2-or 8-months old recipients for 7 days revealed that neither lymphocytes from old donors or old recipients were capable of inducing the appearance of anti-Id-blocked, hapten-augmentable PFC in the lymphocyte population from 2-month-old donors. Thus, the results of this study indicate syner-gistic co-operation between B lymphocytes and thymus cells from old donors for the production of auto-anti-idiotypic antibody regulation with age. This production of auto-anti-Id antibody with age seems not to be an induced maturation event but perhaps an intrinsic property unique to lymphocytes from old donors.

Résumé

L'effet du vieillissement sur la capacité des lymphocytes B et des cellules thymiques en provenance de sujets d'âges divers aptes à produire des cellules formant plaques (PFC) augmentables à l'hapten et inhibées par l'action d'un anti-idotype a été étudiée à l'aide de techniques de transfert de cellules d'adoption. Des souris ayant reçu une dose de radiation létale ont ensuite été rétabalies à aide de lymphocytes B et de cellules thymiques syngéniqucs provenant de sujets d'âges divers. Les donataires ont été immunisés à l'immunoglobinc G bovine trinitrophénolée (TNP-BGG) soit un, soit sept jours après le transfert. Quatorze jours après l'immunisation, les réponses des PFC IgG anti-TNP splénétiques en présence et en l'absence d'hapten ont été évaluées. Les résultats ont montré que la population des lymphocytes B provenant des sujets âgés de moins de 2 mois agissant en coopération avec les cellules thymiques provenant de sujets âgés de 2 à 19 mois étaient incapables de rétablir la production de PFC aumentables à l'hapten inhibées par l'anti-Id. Des résultats analogues ont été obtenus lors du rétablissement des donataires à l'aide de cellules thymiques provenant de sujets âgés de 2 mois en collaboration avec les cellules B provenant de sujets âgés de 2 à 24 mois. Au contraire, les souris rétablies à l'aide de cellules B et de cellules thymiques en provenance du même sujet âgé de 8 mois ou plus accusaient un taux de PFC augmentables à l'hapten sensiblement plus élevé. Les souris établies à l'aide de cellules B provenant de sujets âgés de 8 mois ou plus et de cellules thymiques provenant de sujets âgés de 8 à 19 mois présentaient également un taux élevé de PFC augmentables à l'hapten. Les expériences sur les cellules B et les cellules thymiques provenant de sujets âgés de 2 à 8 mois introduites pendant 7 jours dans des destinataires âgés de 2 à 8 mois ayant reçu une dose de radiation létale ont montré que ni les lymphocytes en provenance de donateurs âgés ni celles des donataires âgés étaient capables de faire apparaître des PFC augmentables à l'hapten inhibées par l'anti-Id dans la population de lymphocytes provenant de sujets âgés de 2 mois. Les résultats de cette étude indiquent donc une collaboration synergétique entre les lymphocytes B et les cellules thymiques en provenance de donateurs âgés en vue de la production de la régulation auto-anti-idiotypique avec l'âge. Cette production d'anticorps auto-anti-Id avec l'âge ne semble pas due à la maturation, mais paraît être une propriété intrinsèque des lymphocytes provenant de sujets âgés.

Type
Articles
Information
Canadian Journal on Aging / La Revue canadienne du vieillissement , Volume 1 , Issue 1-2 , September 1982 , pp. 3 - 10
Copyright
Copyright © Canadian Association on Gerontology 1982

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