Background: Gender disparities endure in neurosurgery, impacting the experiences of female practitioners. Unveiling these challenges is crucial for promoting inclusivity and addressing the unique obstacles faced by women in the field. Methods: An international survey designed using a physician wellness framework was sent to neurosurgeons between June 2021 and November 2021. Univariate analysis (Kruskal-Wallis Test) was performed to assess feelings of inclusion and diversity as a function of gender. Results: Of the total 384 respondents (65% neurosurgeons, 6% fellows, and 29% residents), 71% identified as male, 27% as female, and 2% as other. Compared to their male colleagues, female neurosurgeons more strongly endorsed feeling that their career progression has been limited by their gender (p<0.05) and were less likely to feel entrusted in their surgical ability (p<0.05) or to have equal access to surgical resources (p<0.05). Furthermore, they were less likely to endorse feelings that leaders in their department were committed to creating an inclusive environment (p<0.05). Conclusions: Our survey sheds light on significant gender-related disparities in neurosurgery. Female neurosurgeons express heightened concerns about gender-limiting career progression, reduced trust in their surgical abilities, and disparities in resource access. These findings underscore the imperative to foster a more inclusive and supportive environment within the field.