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NMDA Receptor Blockade and Spinal Cord Ischemia Due to Aortic Crossclamping in the Rat Model

Published online by Cambridge University Press:  18 September 2015

F. Follis ,
K. Miller ,
O. U. Scremin ,
S. Pett ,
R. Kessler  and
J. Wernly
F. Follis*
Affiliation:
Department of Thoracic and Cardiothoracic Surgery, University of California Los Angeles School of Medicine, Los Angeles
K. Miller
Affiliation:
Department of Thoracic and Cardiothoracic Surgery, University of California Los Angeles School of Medicine, Los Angeles
O. U. Scremin
Affiliation:
University of New Mexico and West Los Angeles V.A. Medical Center and Department of Physiology, University of California Los Angeles School of Medicine, Los Angeles
S. Pett
Affiliation:
Department of Thoracic and Cardiothoracic Surgery, University of California Los Angeles School of Medicine, Los Angeles
R. Kessler
Affiliation:
Department of Thoracic and Cardiothoracic Surgery, University of California Los Angeles School of Medicine, Los Angeles
J. Wernly
Affiliation:
Department of Thoracic and Cardiothoracic Surgery, University of California Los Angeles School of Medicine, Los Angeles
*
University of New Mexico, Department of Thoracic and Cardiovascular Surgery, 2211 Lomas Blvd., Albuquerque, New Mexico, USA 87131
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Abstract:

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Recent brain research proposes that, during ischemia, synaptically released excitatory amino acid neurotransmitters accumulate at toxic concentrations with ensuing neuronal death. Their action is mediated by the receptor subtype N-methyl-D-aspartate (NMDA). The protective effect of NMDA receptor blockade with intrathecal MgS04 and MK-801 was investigated during spinal cord ischemia induced by aortic occlusion of 12 minutes. Male Sprague-Dawley rats, 250-300g, underwent intrathecal administration of 20 μL of normal saline (SA n = 16), MgS04 1M (MG n = 16), or MK-801, 25 mM solutions (MK n = 16) in a randomized order. After 2 hours, the animals underwent occlusion of the thoracic aorta and subclavian arteries for 12 min. An additional control group (CO n = 16) underwent occlusion for 12 minutes, without intrathecal injection. The animals were scored according to their functional performance (LS = lesion score) each day for four days by a blinded observer. Mean LS were calculated for each group at a given day. Treatment and control groups were not different at day 1 (P = 0.302). Group MG was improved from groups SA (P = < 0.0039) and CO (P = < 0.0048) at day 4. This study demonstrates that although intrathecal NMDA receptor blockade with MgS04 or MK-801 does not prevent paraplegia due to spinal cord ischemia in the rat, it could however influence the rate of recovery after ischemic injury.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 21 , Issue 3 , August 1994 , pp. 227 - 232
Copyright
Copyright © Canadian Neurological Sciences Federation 1994

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