Hostname: page-component-848d4c4894-2pzkn Total loading time: 0 Render date: 2024-05-11T12:50:10.769Z Has data issue: false hasContentIssue false
  • English
  • Français

Localization of Nestin in Amygdaloid Kindled Rat: An Immunoelectron Microscopic Study

Published online by Cambridge University Press:  02 December 2014

Toshitaka Nakagawa ,
Osamu Miyamoto ,
Najma A. Janjua ,
Roland N. Auer ,
Seigo Nagao  and
Toshifumi Itano
Toshitaka Nakagawa
Affiliation:
Departments of Neurobiology, Kagawa Medical University, Miki, Kagawa, Japan
Osamu Miyamoto
Affiliation:
Departments of Neurobiology, Kagawa Medical University, Miki, Kagawa, Japan
Najma A. Janjua
Affiliation:
Departments of Neurobiology, Kagawa Medical University, Miki, Kagawa, Japan
Roland N. Auer
Affiliation:
Departments of Pathology and Clinical Neurosciences, Neuroscience Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada
Seigo Nagao
Affiliation:
Department of Neurological Surgery, Kagawa Medical University, Miki, Kagawa, Japan
Toshifumi Itano
Affiliation:
Departments of Neurobiology, Kagawa Medical University, Miki, Kagawa, Japan
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Background:

Nestin is a class VI intermediate filament protein, expressed during early embryonic development in mammals. Postnatally, nestin and its mRNA are down-regulated and gradually disappear. Recently, nestin expression has been detected in the adult nervous system, and it has been suggested that this protein may be related to neurogenesis, although, its role in the mechanism of neurogenesis is not known.

Methods:

The present study examined the localization of nestin in CNS tissue of the amygdaloid kindled rat by light and electron microscopy.

Results:

Kindled animals showed nestin expression mainly in the piriform cortex and the perirhinal cortex. By light microscopy, nestin was shown to be expressed in astrocytes, neurons, and endothelial cells. Electron microscopy demonstrated nestin expression in endothelial cells, astrocytic perivascular end feet, the rare pericyte, neurons and oligodendrocytes.

Conclusion:

We conclude that epilepsy causes widespread nestin expression in many cell types in the CNS, including non-neural cells.

Type
Other
Information
Canadian Journal of Neurological Sciences , Volume 31 , Issue 4 , November 2004 , pp. 514 - 519
Copyright
Copyright © The Canadian Journal of Neurological 2004

References

1. Kuhn, HG, Dickinson-Anson, H, Gage, FH. Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation. J Neurosci 1996;16:2027–2033.Google Scholar
2. Kaneko, Y, Sakakibara, S, Imai, T, et al. Musashi1: an evolutionally conserved marker for CNS progenitor cells including neural stem cells. Dev Neurosci 2000;22:139–153.Google Scholar
3. Racine, RJ. Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol 1972;32:281–294.Google Scholar
4. Umeoka, S, Miyamoto, O, Janjua, NA, Nagao, S, Itano, T. Appearance and alteration of TUNELpositive cells through epileptogenesis in amygdaloid kindled rat. Epilepsy Res 2000;42:97–103.CrossRefGoogle Scholar
5. Clark, M, Post, RM, Weiss, SRB, Cain, CJ, Nakajima, T. Regional expression of c-fos mRNA in rat brain during the evolution of amygdala kindled seizures. Mol Brain Res 1991;11:54–64.Google Scholar
6. Mohapel, P, Corcoran, ME. Kindling antagonism: interactions of the amygdala with the piriform, perirhinal, and insular cortices. Brain Res 1996;733:211–218.CrossRefGoogle ScholarPubMed
7. Loscher, W, Ebert, U. The role of the piriform cortex in kindling. Prog Neurobiol 1996;50:427–481.Google Scholar
8. Li, Y, Chopp, M. Temporal profile of nestin expression after focal cerebral ischemia in adult rat. Brain Res 1999;838:1–10.CrossRefGoogle ScholarPubMed
9. Duggal, N, Hammond, RR. Nestin expression in ganglioglioma. Exp Neurol 2002;174:89–95.Google Scholar
10. Holmin, S, Almqvist, P, Lendahl, U, Mathiesen, T. Adult nestin-expressing subependymal cells differentiate to astrocytes in response to brain injury. Eur J Neurosci 1997;9:65–75.Google Scholar
11. Frisen, J, Johansson, CB, Torok, C, Risling, M, Lendahl, U. Rapid, widespread, and longlasting induction of nestin contributes to the generation of glial scar tissue after CNS injury. J Cell Biol 1995;131:453–464.Google Scholar
12. Tohyama, T, Lee, VM, Rorke, LB, et al. Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells. Lab Invest 1992;66:303–313.Google Scholar
13. Kobayashi, M, Sjoberg, G, Soderhall, S, et al. Pediatric rhabdomyosarcomas express the intermediate filament nestin. Pediatr Res 1998;43:386–392.Google Scholar
14. Krum, JM, Rosenstein, JM. Transient coexpression of nestin, GFAP, and vascular endothelial growth factor in mature reactive astroglia following neural grafting or brain wounds. Exp Neurol 1999;160:348–360.Google Scholar
15. Umeoka, S, Miyamoto, O, Nakagawa, T, et al. Expression of an embryonic intermediate filament protein in amygdaloid kindled rats. Epilepsy Res 2001;43:249–253.CrossRefGoogle ScholarPubMed
16. Miyamoto, O, Itano, T, Yamamoto, Y, et al. Effect of embryonic hippocampal transplantation in amygdaloid kindled rat. Brain Res 1993;603:143–147.Google Scholar
17. McNamara, JO. The neurobiological basis of epilepsy. Trends Neurosci 1992;15:357–359.Google Scholar
18. Minamoto, Y, Itano, T, Tokuda, M, et al. In vivo microdialysis of amino acid neurotransmitters in the hippocampus in amygdaloid kindled rat. Brain Res 1992;573:345–348.Google Scholar
19. Whittemore, SR, Morassutti, DJ, Walters, WM, Liu, RH, Magnuson, DS. Mitogen and substrate differentially affect the lineage restriction of adult rat subventricular zone neural precursor cell populations. Exp Cell Res 1999;252:75–95.Google Scholar
20. Sahin Kaya, S, Mahmood, A, Li, Y, Yavuz, E, Chopp, M. Expression of nestin after traumatic brain injury in rat brain. Brain Res 1999;840:153–157.CrossRefGoogle ScholarPubMed
21. Tropepe, V, Sibilia, M, Ciruna, BG, et al. Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Dev Biol 1999;208:166–188.Google Scholar
22. Dahlstrand, J, Lardelli, M, Lendahl, U. Nestin mRNA expression correlates with the central nervous system progenitor cell state in many, but not all, regions of developing central nervous system. Dev Brain Res 1995;84:109–129.Google Scholar
23. Tohyama, T, Lee, VM, Rorke, LB, et al. Monoclonal antibodies to a rat nestin fusion protein recognize a 220-kDa polypeptide in subsets of fetal and adult human central nervous system neurons and in primitive neuroectodermal tumor cells. Am J Pathol 1993;143:258–268.Google ScholarPubMed
24. Hockfield, S, McKay, RD. Identification of major cell classes in the developing mammalian nervous system. J Neurosci 1985;5:3310–3328.Google Scholar
25. Lendahl, U, Zimmerman, LB, McKay, RD. CNS stem cells express a new class of intermediate filament protein. Cell 1990;60:585–595.Google Scholar
26. Reynolds, BA, Tetzlaff, W, Weiss, S. A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes. J Neurosci 1992;12:4565–4574.Google Scholar
27. Hosokawa, J, Itano, T, Usuki, T, et al. Morphological changes in the hippocampus in amygdaloid kindled mouse. Epilepsy Res 1995;20:11–20.Google Scholar
28. Quinn, SM, Walters, WM, Vescovi, AL, Whittemore, SR. Lineage restriction of neuroepithelial precursor cells from fetal human spinal cord. J Neurosci Res 1999;57:590–602.Google Scholar
29. Palmer, TD, Willhoite, AR, Gage, FH. Vascular niche for adult hippocampal neurogenesis. J Comp Neurol 2000;425:479–494.Google Scholar
30. Goddard, GV, McIntyre, DC, Leech, CK. A permanent change in brain function resulting from daily electrical stimulation. Exp Neurol 1969;25:295–330.Google Scholar
31. Svendsen, CN, Smith, AG. New prospects for human stem-cell therapy in the nervous system. Trends Neurosci 1999;22:357–364.CrossRefGoogle ScholarPubMed
32. Craig, CG, D’sa, R, Morshead, CM, Roach, A, van der Kooy, D. Migrational analysis of the constitutively proliferating subependyma population in adult mouse forebrain. Neuroscience 1999;93:1197–1206.Google Scholar
33. Mokry, J, Nemecek, S. Cerebral angiogenesis shows nestin expression in endothelial cells. Gen Physiol Biophys 1999;18 Suppl 1:25–29.Google ScholarPubMed
34. Luskin, MB, Boone, MS. Rate and pattern of migration of lineally-related olfactory bulb interneurons generated postnatally in the subventricular zone of the rat. Chem Senses 1994;19:695–714.Google Scholar