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Immunocytochemical Analysis of Intermediate Filaments in Human Ependymal Tumors

Published online by Cambridge University Press:  18 September 2015

D. Izukawa  and
B. Lach
D. Izukawa
Affiliation:
Department of Surgery, Division of Neurosurgery, Ottawa Civic Hospital and University of Ottawa, Ottawa
B. Lach*
Affiliation:
Department of Laboratory Medicine (Neuropathology), Ottawa Civic Hospital and University of Ottawa, Ottawa
*
Department of Laboratory Medicine (Neuropathology), Ottawa Civic Hospital, 1053 Carling Ave., Ottawa, Ontario, Canada K1Y 4E9
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Abstract:

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The peroxidase anti-peroxidase technique was used for localization of glial fibrillary acidic protein (GFAP) and vimentin (VM) in 19 ependymal tumors in order to determine if a unique pattern of intermediate filament (IF) expression could be demonstrated. Cytokeratin (CK) immunoreactivity was examined in a subgroup of 7 tumors with papillary pattern. Nineteen non-ependymal neuroectodermal tumors were used as controls. Ependymomas, subependymomas and astrocytomas were positive for both IF. Oligodendrogliomas, oligodendroglial portions of mixed gliomas and the majority of medulloblastomas were negative for GFAP and VM. Areas of poor differentiation in all tumors demonstrated little expression of any IF. A composite ependymoma/choroid plexus papilloma showed the presence of GFAP, VM and CK in the papillomatous portion only. Four papillary ependymomas were negative for CK. This study emphasizes the parallel distribution of GFAP and VM in well differentiated ependymomas and other glial tumors and casts doubt upon the concept of VM as a marker for de-differentiation in neuroectodermal neoplasia.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 15 , Issue 2 , May 1988 , pp. 114 - 118
Copyright
Copyright © Canadian Neurological Sciences Federation 1988

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