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The Effects of Exercise Intensity on p-NR2B Expression in Cerebral Ischemic Rats

Published online by Cambridge University Press:  02 December 2014

Anjing Zhang ,
Yulong Bai ,
Yongshan Hu ,
Feng Zhang ,
Yi Wu ,
Yang Wang ,
Ping Zheng  and
Qiang He
Anjing Zhang
Affiliation:
Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China
Yulong Bai*
Affiliation:
Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China
Yongshan Hu
Affiliation:
Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China
Feng Zhang
Affiliation:
Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China
Yi Wu
Affiliation:
Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China
Yang Wang
Affiliation:
Department of Anatomy and Embryology, Fudan University, Shanghai, China
Ping Zheng
Affiliation:
State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China
Qiang He
Affiliation:
Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China
*
Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China. Email: doctorbaiyulong@126.com
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Abstract

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Background:

The current study explored the effects of treadmill exercise intensity on functional recovery and hippocampal phospho-NR2B (p-NR2B) expression in cerebral ischemic rats, induced by permanent middle cerebral artery occlusion (MCAO) surgery.

Method:

Adult male Sprague-Dawley rats were randomly divided into four groups, including sham, no exercise (NE), low intensity training (LIT, v = 15 m/min), and moderate intensity training groups (MIT, v = 20 m/min). At different time points, the hippocampal expressions of p-NR2B and total NR2B were examined. In addition, neurological deficit score (NDS), body weight, and 2,3,5-triphenyltetrazolium chloride (TTC) staining were used to evaluate brain infarct volume as assessments of post-stroke functional recovery. In order to investigate the effect of exercise on survival, the mortality rate was also recorded.

Results:

The results showed that treadmill exercise significantly decreased hippocampal expression of p-NR2B but didn't change the total NR2B, compared to the NE group on the 3rd, 7th, and 14th days following MCAO surgery. The effect on changes in p-NR2B levels, body weight, and brain infarct volume were more significant in the LIT compared to the MIT group.

Discussion and Conclusion:

The current findings demonstrate that physical exercise can produce neuroprotective effects, in part by down-regulating p-NR2B expression. Furthermore, the appropriate intensity of physical exercise is critical for post-stroke rehabilitation.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 39 , Issue 5 , September 2012 , pp. 613 - 618
Copyright
Copyright © The Canadian Journal of Neurological 2012

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