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Decrease in the Rate of Protein Synthesis by Polysomes from Cultured Fibroblasts of Patients and Carriers with Duchenne Muscular Dystrophy

Published online by Cambridge University Press:  18 September 2015

M. Boulé ,
M. Vanasse  and
L. Brakier-Gingras
M. Boulé
Affiliation:
Département de Biochimie, Université de Montréal et Section de Neurologie, Hôpital Sainte-Justine Montréal, Québec, Canada
M. Vanasse
Affiliation:
Département de Biochimie, Université de Montréal et Section de Neurologie, Hôpital Sainte-Justine Montréal, Québec, Canada
L. Brakier-Gingras*
Affiliation:
Département de Biochimie, Université de Montréal et Section de Neurologie, Hôpital Sainte-Justine Montréal, Québec, Canada
*
Département de Biochimie, Université de Montréal, H3C 3J7, Montréal, Québec, Canada
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Polysomes extracted from cultured fibroblast cells isolated from patients with Duchenne muscular dystrophy (DMD), carriers of the disease, and normal controls were used for in vitro measurement of protein synthesis in a wheat germ extract system. It was observed that polysomes from patients and carriers (seven of each aged 17 years or older) exhibited a 3-fold and a 1.5-fold decrease in the rate of protein synthesis, respectively, as compared with controls. These results are discussed with a view to developing a sensitive and easily available assay for the detection of DMD carriers.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 6 , Issue 3 , August 1979 , pp. 355 - 358
Copyright
Copyright © Canadian Neurological Sciences Federation 1979

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