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The Cycling Pool of Cells within Human Brain Tumors: In Situ Cytokinetics Using the Monoclonal Antibody Ki-67

Published online by Cambridge University Press:  18 September 2015

Ana Maria Tsanaclis ,
Françoise Robert ,
Jean Michaud  and
Steven Brem
Ana Maria Tsanaclis
Affiliation:
Lady Davis Institute for Medical Research and the Department of Neuroscience, Sir Mortimer B. Davis-Jewish General Hospital, Montréal, Québec Department of Neurology and Neurosurgery, McGill University, Montréal, Québec
Françoise Robert
Affiliation:
Department of Pathology, Sir Mortimer B. Davis-Jewish General Hospital, Montréal, Québec Department of Pathology, University of Montréal, Montréal, Québec
Jean Michaud
Affiliation:
Department of Pathology, Ste. Justine Hospital, Montréal, Québec Department of Pathology, University of Montréal, Montréal, Québec
Steven Brem*
Affiliation:
Lady Davis Institute for Medical Research and the Department of Neuroscience, Sir Mortimer B. Davis-Jewish General Hospital, Montréal, Québec Department of Oncology, Sir Mortimer B. Davis-Jewish General Hospital, Montréal, Québec Department of Neurology and Neurosurgery, McGill University, Montréal, Québec
*
Neurosurgical Oncology, 233 East Erie Street, Suite 500, Chicago, Illinois, U.S.A. 60611-2906
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Abstract:

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Brain tumor growth results from the relative proportion of cells contained in three populations: a) cycling/proliferative; b) quiescent (Go)/static, and c) terminally differentiated/dying. The cycling compartment can be detected by the mouse monoclonal Ki-67 antibody, an available, rapid, safe, sensitive, and specific method for immunostaining of proliferative cells. We report the Ki-67 labeling index (LI) in 48 brain tumors. Malignant brain tumors have elevated Lis, ranging from 6.0% to 56.9%: anaplastic astrocytoma, 8.0 ± 7.3; glioblastoma multiforme, 10.1 ±4.2; germinoma, 11.7; medulloblastoma, 13.1 ± 6.6; metastases, 40.3 ± 13.1. By contrast, slow-growing tumors showed lower values (P < .001), approaching 1%: acoustic schwannoma, 0.4 ± 0.6; pituitary adenoma, 1.3 ± 1.9; meningioma, 1.2 ± 1.2; low-grade astrocytoma, < 1; pilocytic astrocytoma, 5.6. Human brain tumors can therefore be ranked according to the percentage of cycling cells with the acoustic schwannoma among the least proliferative and the metastatic carcinoma among the most proliferative. Within a given histotype, the Ki-67 LI may have prognostic and therapeutic implications for the individual patient. Already important for neuro-oncology research, the Ki-67 labeling index should be added to the armamentarium of the clinical neuropathologist to complement the standard histopathologic diagnosis with a cytokinetic analysis of cellular proliferation.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 18 , Issue 1 , February 1991 , pp. 12 - 17
Copyright
Copyright © Canadian Neurological Sciences Federation 1991

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