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Crown of Microfilaments in the Extending Cytoplasmic Processes of Medulloblastoma Glial Progenitors

Published online by Cambridge University Press:  18 September 2015

Bernard L. Maria ,
Robert Cumming  and
Loretta Sukhu
Bernard L. Maria*
Affiliation:
Division of Neurology (Department of Pediatrics), University of Florida College of Medicine, Gainesville, Florida
Robert Cumming*
Affiliation:
Division of Neurology (Department of Pediatrics), University of Florida College of Medicine, Gainesville, Florida
Loretta Sukhu*
Affiliation:
Division of Neurology (Department of Pediatrics), University of Florida College of Medicine, Gainesville, Florida
*
Division of Pediatric Neurology, University of Florida College of Medicine, Gainesville, Florida, U.S.A. 32610–0296
Division of Pediatric Neurology, University of Florida College of Medicine, Gainesville, Florida, U.S.A. 32610–0296
Division of Pediatric Neurology, University of Florida College of Medicine, Gainesville, Florida, U.S.A. 32610–0296
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Abstract:

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Microfilaments and microtubules play a part in the extension of neuronal processes but their roles in the formation of glial processes have not yet been determined. The objectives of this study were to determine the organization of microfilaments in differentiating glial progenitors (RB2 cells) and to study the effects of microfilament or microtubule disruption on process extension. Intense F-actin staining (crown of microfilaments) was observed at the leading edge of a small extending conical tip in differentiating RB2 cells, but was absent in process-bearing TE671 rhabdomyosarcoma cells. No significant difference was noted in the mean number of TE671 cells with processes treated with a microfilament disrupter from that of similarly treated controls. In contrast, a significant difference was noted in the mean number of RB2 cells with processes after microfilament disruption treatment from that of similarly treated controls. Microtubule disruption arrested extension and caused process retraction in both cell types. The results of this study demonstrate that microtubules play an equally important part in the extension and stabilization of the RB2 and TE671 processes. Moreover, the crown of microfilaments concentrated in the glial RB2 process (and not in the TE671 process) may be critical to its extension during differentiation.

Type
Articles
Information
Canadian Journal of Neurological Sciences , Volume 19 , Issue 1 , February 1992 , pp. 23 - 33
Copyright
Copyright © Canadian Neurological Sciences Federation 1992

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