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Pretranslational regulation of the expression of the lipoprotein lipase (EC 3.1, l.34) gene by dietary fatty acids in the rat

Published online by Cambridge University Press:  02 April 2007

M. C. Murphy ,
A. Zampelas ,
S. M. Puddicombe ,
N. P. Furlonger ,
L. M. Morgan  and
C. M. Williams
M. C. Murphy
Affiliation:
Nutritional Metabolism Research Group, School of Biological Sciences, University of Surrey, Guildford GU2 5XH
A. Zampelas
Affiliation:
Nutritional Metabolism Research Group, School of Biological Sciences, University of Surrey, Guildford GU2 5XH
S. M. Puddicombe
Affiliation:
Nutritional Metabolism Research Group, School of Biological Sciences, University of Surrey, Guildford GU2 5XH
N. P. Furlonger
Affiliation:
Nutritional Metabolism Research Group, School of Biological Sciences, University of Surrey, Guildford GU2 5XH
L. M. Morgan
Affiliation:
Nutritional Metabolism Research Group, School of Biological Sciences, University of Surrey, Guildford GU2 5XH
C. M. Williams
Affiliation:
Nutritional Metabolism Research Group, School of Biological Sciences, University of Surrey, Guildford GU2 5XH
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Abstract

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Although there have been a number of studies of effects of diet and hormones on lipoprotein lipase (EC 3.1.1.34; LPL) activity and levels of LPL mRNA (Raynolds et al. 1990), there have been no studies which have investigated effects of different dietary fatty acids on LPL gene expression. In the present study male Wistar Albino rats were pair-fed diets containing 50 g fat/kg of different fatty acid composition for 2 weeks. The diets fed were (1) a mixed oil (450 g saturated fatty acids, 420 g monounsaturated fatty acids, 130 g polyunsaturated fatty acids/kg; n 8), (2) maize oil (n 8), or (3) fish oil (n 8). Animals were killed, RNA was extracted from liver and perirenal and epididymal fat pads, and analysed by ‘Northern methodology’. Samples were hybridized to a human cDNA probe for LPL (Gotoda et al. 1989). Two transcripts were identified in epididymai and perirenal adipose tissue which were approximately 3·7 and 1·7 kb in size. The results suggested that (1) fish oil-fed animals had significantly greater production of LPL mRNA in epididymai adipose tissue compared with maize oil-fed animals (P < 0·05), (2) maize oil-fed animals had significantly greater production of LPL mRNA in perirenal fat compared with the other dietary groups (P < 0·05), (3) expression in the liver was not significant. Rats fed on a fish oil diet had significantly reduced plasma triacylglycerol concentrations compared with the mixed-oil group (P < 0·05), but there were no significant differences in plasma cholesterol. The differences in LPL could not be explained directly by the changes in plasma immunoreactive-insulin and glucose-dependent insulinotrophic polypeptide levels in the three groups.

Keywords

Lipoprotein lipaseFatty acidsFish oilsRat
Type
Lipid Metabolism
Information
British Journal of Nutrition , Volume 70 , Issue 3 , November 1993 , pp. 727 - 736
Copyright
Copyright © The Nutrition Society 1993

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