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Postprandial lipaemia is exacerbated in fat-cholesterol-fed rabbits: relationship to atheroma deposition

Published online by Cambridge University Press:  09 March 2007

Christine Juhel ,
Christophe Dubois ,
Michele Senft ,
Emile Levy ,
Huguette Lafont  and
Denis Lairon
Christine Juhel
Affiliation:
Unité 130-INSERM (Institut National de la Santé et de la Recherche Médicale), 13009 Marseille, France
Christophe Dubois
Affiliation:
Unité 130-INSERM (Institut National de la Santé et de la Recherche Médicale), 13009 Marseille, France
Michele Senft
Affiliation:
Unité 130-INSERM (Institut National de la Santé et de la Recherche Médicale), 13009 Marseille, France
Emile Levy
Affiliation:
Höpital Sainte-Justine, Centre de Recherche Pédiatrique, Gastroentérologie-Nutrition, Montréal, Québec, H3T IC5, Canada
Huguette Lafont
Affiliation:
Unité 130-INSERM (Institut National de la Santé et de la Recherche Médicale), 13009 Marseille, France
Denis Lairon
Affiliation:
Unité 130-INSERM (Institut National de la Santé et de la Recherche Médicale), 13009 Marseille, France
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Abstract

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The aim of the present study was to evaluate the links between chronic fat-cholesterol intake, postprandial lipaemia and atherogenesis in New Zealand White rabbits. Adult rabbits were fed on either a low-fat control diet (LF) or a high-fat, high-cholesterol diet (HF). Rabbits received a test meal containing [3H]cholesterol and [14C]triolein on days 0 and 63 for the LF group and days 14,28, 42,63 and 84 for the HF group. Blood was collected 24h post-absorptively and 10,24,34 and 48h after test-meal intake. Post-absorptive as well as postprandial lipoproteins and lipaemia were not modified in the LF rabbits, who did not show any atheroma deposition on day 119. In HF rabbits, postprandial plasma triacylglycerols peaked 24–34h after meal intake. The 0-48 h areas under the curves of triacylglycerol andtriacylglycerol-rich lipoproteins (TRL) steadily increased with time of chronic lipid feeding with values significantly higher than those in the LF rabbits. The postprandial plasma and TRL concentrations of dietary radiolabelled lipids were significantly higher in HF than LF rabbits. Post-heparin lipoprotein lipase (EC 3.1.1.34) and hepatic lipase (EC 3.1.1.3) activities were twofold higher in HF rabbits than in LF rabbits at day 105. In HF rabbits, a marked atheroma plaque deposition in the aorta was observed (30·9 (SE 3·9) % of total surface). The extent of atheroma deposition was positively correlated to the postprandial responses of plasma total triacylglycerols and dietary-derived lipids as well as total cholesterol and dietary-derived cholesterolin HF rabbits. In conclusion, chronic ingestion of a HF diet led to marked increases in postprandial lipaemia and TRL particles, and atheroma deposition.

Keywords

Postprandial lipaemiaDietary lipidsHypercholesterolaemic rabbits
Type
General Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 2 , August 1997 , pp. 301 - 311
Copyright
Copyright © The Nutrition Society 1997

References

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