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Occurrence and biochemical characteristics of natural bioactive substances in bovine milk lipids

Published online by Cambridge University Press:  09 March 2007

Joachim Molkentin
Joachim Molkentin*
Affiliation:
Institute for Chemistry and Physics, Federal Dairy Research Centre, PO Box 6069, D-24121 Kiel, Germany
*
*Corresponding author: J. Molkentin, tele, fax +49 431 6092300, email molkentin@bafm.de
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Abstract

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Bovine milk lipids (BML) contain a number of bioactive substances with positive as well as negative properties, mainly in the class of fatty acids. Besides trans fatty acids (TFA), conjugated linoleic acids (CLA) are of particular interest. Apart from ruminant meat products the main source of CLA in food are BML. Although TFA as well as saturated fatty acids (12:0–16:0) are thought to be positively correlated with atherosclerosis and coronary heart disease, CLA are considered antiatherogenic. Further, CLA are reported to reduce adipose fat and to have anticarcinogenic properties. The varying CLA and TFA contents of lipids from milk and dairy products are positively correlated with one another. However, TFA are also negatively correlated with 12:0–16:0 in BML. Anticarcinogenic effects are also ascribed to butyric acid as well as to some phospholipids and ether lipids present in BML. Moreover, the essential fatty acids 18:2 n-6 and 18:3 n-3 are found in BML which are involved in a variety of biochemical processes and thus have numerous functions in human metabolism. Contents of the individual bioactive components of BML are summarised taking into account also seasonal variations. The total content of bioactive substances in BML is approximately 75 % but their overall impact on human health considering benefits and drawbacks is difficult to assess.

Keywords

Bioactive substancesBovine milk fatTrans fatty acidsConjugated linoleic acid
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue S1 , November 2000 , pp. 47 - 53
Copyright
Copyright © The Nutrition Society 2000

References

Adolf, T, Eberhardt, W, Heseker, H, Hartmann, S, Herwig, A, Matiaske, B, Moch, KJ, Schneider, R, Kübler, W (1994) Lebensmittel- und Nährstoffaufnahme in der Bundesrepublik Deutschland. In VERA-Schriftenreihe, Band XII [W, Kübler, HJ, Anders and and W, Heeschen, editors]. Niederkleen: Wissenschaftlicher Fachverlag Dr. Fleck.Google Scholar
Belury, MA (1995) Conjugated dienoic linoleate: a polyunsaturated fatty acid with unique chemopreventive properties. Nutrition Reviews 53, 83–89.CrossRefGoogle Scholar
Berdel, WE (1991) Membrane-interactive lipids as experimental anticancer drugs. British Journal of Cancer 64, 208–211.CrossRefGoogle ScholarPubMed
Berry, EM, Eisenberg, S, Haratz, D, Friedlander, Y, Norman, Y, Kaufmann, NA and & Stein, Y (1991) Effects of diets rich in monounsaturated fatty acids on plasma lipoproteins – The Jerusalem Nutrition Study: high MUFAs vs high PUFAs. American Journal of Clinical Nutrition 53, 899–907.CrossRefGoogle Scholar
Beynen, AC, Katan, MB (1989) Impact of dietary cholesterol and fatty acids on serum lipids and lipoproteins in man. In Role of Fats in Human Nutrition, pp. 237–286 [Vergroesen, AJ and Crawford, M, editors]. London: Academic Press.CrossRefGoogle Scholar
Bonanome, A and & Grundy, SM (1988) Effect of dietary stearic acid on plasma cholesterol and lipoprotein levels. New England Journal of Medicine 318, 1244–1248.CrossRefGoogle ScholarPubMed
Chan, JK, Bruce, VM and & McDonald, BE (1991) Dietary ω3-linolenic acid is as effective as oleic acid and linoleic acid in lowering blood cholesterol in normolipidemic men. American Journal of Clinical Nutrition 53, 1230–1234.CrossRefGoogle Scholar
Chen, ZX and & Breitman, TR (1994) Tributyrin: a prodrug of butyric acid for potential clinical application in differentiation therapy. Cancer Research 54, 3494–3499.Google ScholarPubMed
Chen, ZY, Chan, PT, Kwan, KY and & Zhang, A (1997) Reassessment of the antioxidant activity of conjugated linoleic acids. Journal of the American Oil Chemists' Society 74, 749–753.CrossRefGoogle Scholar
Chin, SF, Liu, W, Storkson, JM, Ha, YL and & Pariza, MW (1992) Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. Journal of Food Composition and Analysis 5, 185–197.CrossRefGoogle Scholar
Chin, SF, Storkson, JM, Albright, KJ, Cook, ME and & Pariza, MW (1994) Conjugated linoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed efficiency. Journal of Nutrition 124, 2344–2349.CrossRefGoogle ScholarPubMed
Christie, WW, Noble, RC and & Davies, G (1987) Phospholipids in milk and dairy products. Journal of the Society of Dairy Technology 40, 10–12.CrossRefGoogle Scholar
Cohen, LA, Thompson, DO, Maeura, Y, Choi, K, Blank, ME and & Rose, DP (1986) Dietary fat and mammary cancer. I. Promoting effects of different dietary fats on N-nitrosomethylurea-induced rat mammary tumorigenesis. Journal of the National Cancer Institute 77, 33–42.Google ScholarPubMed
Cook, MC, Miller, CC, Park, Y and & Pariza, M (1993) Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poultry Science 72, 1301–1305.CrossRefGoogle ScholarPubMed
DeMan, L and & DeMan, JM (1983) Trans fatty acids in milk fat. Journal of the American Oil Chemists' Society 60, 1095–1098.CrossRefGoogle Scholar
Dhiman, TR, Anand, GR, Satter, LD and & Pariza, M (1996) Conjugated linoleic acid content of milk from cows fed different diets Journal of Dairy Science 79 S1, 35.Google Scholar
Diomede, L, Colotta, F, Piovani, B, Re, F, Modest, EJ & Salmona, M (1993) Induction of apoptosis in human leukemic cells by the ether lipid 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine. A possible basis for its selective action. International Journal of Cancer 53, 124–130.CrossRefGoogle ScholarPubMed
Dugan, MER, Aalhus, JL, Schaefer, AL and & Kramer, JKG (1997) The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Canadian Journal of Animal Science 77, 723–725.CrossRefGoogle Scholar
Dupont, J, White, PJ and & Feldman, EB (1991) Saturated and hydrogenated fats in food in relation to health. Journal of the American College of Nutrition 10, 577–592.CrossRefGoogle ScholarPubMed
Emken, EA (1984) Nutrition and biochemistry of trans and positional fatty acid isomers in hydrogenated oils. Annual Review of Nutrition 4, 339–376.CrossRefGoogle ScholarPubMed
Enig, MG, Munn, RJ and & Keeney, M (1978) Dietary fat and cancer trends – a critique. Federal Proceedings 37, 2215–2220.Google Scholar
Fogerty, AC, Ford, GL and & Svoronos, D (1988) Octadeca-9,11-dienoic acid in foodstuffs and in the lipids of human blood and breast milk. Nutrition Reports International 38, 937–944.Google Scholar
Frede, E, Precht, D & Timmen, H (1990) Lipide: Fettsäuren, Fette und Fettbegleitstoffe einschlieβlich fettlöslicher Vitamine. In Kompendium zur milchwirtschaftlichen Chemie, pp. 57–78 [Schlimme, E, editors]. München: Volkswirtschaftlicher Verlag.Google Scholar
Fritsche, J and & Steinhart, H (1998) Amounts of conjugated linoleic acid (CLA) in German foods and evaluation of daily intake. Zeitschrift für Lebensmittel-Untersuchung und -Forschung A 206, 77–82.CrossRefGoogle Scholar
Gray, IK (1973) Seasonal variations in the composition and thermal properties of New Zealand milk fat. Journal of Dairy Research 40, 207–214.CrossRefGoogle ScholarPubMed
Grundy, SM and & Denke, MA (1990) Dietary influences on serum lipids and lipoproteins. Journal of Lipid Research 31, 1149–1172.CrossRefGoogle ScholarPubMed
Ha, YL, Grimm, NK and & Pariza, MW (1987) Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8, 1881–1887.CrossRefGoogle ScholarPubMed
Ha, YL, Storkson, JM and & Pariza, MW (1990) Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Research 50, 1097–1101.Google ScholarPubMed
Hallgren, B, Niklasson, A, Stallberg, G and & Thorin, H (1974) On the occurrence of 1-O-alkylglycerols and 1-O(2-methoxyalkyl)glycerols in human colostrum, human milk, cow's milk, sheep's milk, human red bone marrow, red cells, blood plasma and a uterine carcinoma. Acta Chemica Scandinavica B28, 1029–1034.CrossRefGoogle Scholar
Hegsted, DM, McGandy, RB and & Myers, ML (1965) Quantitative effects of dietary fat on serum cholesterol in man. American Journal of Clinical Nutrition 17, 281–295.CrossRefGoogle ScholarPubMed
Henninger, M and & Ulberth, F (1994) Trans fatty acid content of bovine milk fat. Milchwissenschaft 49, 555–558.Google Scholar
Holvoet, P and & Collen, D (1994) Oxidized lipoproteins in atherosclerosis and thrombosis. FASEB Journal 8, 1279–1284.CrossRefGoogle ScholarPubMed
Horrobin, DF (1997) Essentiality of oils and fats for human nutrition and disease. In Modern Developments in Food Lipids, pp. 171–192. [Shukla, VKS and Kochhar, SP, editors]. AarhusDanmark: International Food Science Center/(ISBN 87-894166-3-4).Google Scholar
Hunter, JE & Applewhite, TH (1986) Isomeric fatty acids in the US diet: levels and health perspectives. American Journal of Clinical Nutrition 44, 707–717.CrossRefGoogle ScholarPubMed
Ip, C (1997) Review of the effects of trans fatty acids, oleic acid, n-3 polyunsaturated fatty acids, and conjugated linoleic acid on mammary carcinogenesis in animals. American Journal of Clinical Nutrition 66, (Suppl), 1523S-1529S.CrossRefGoogle ScholarPubMed
Ip, C, Chin, SF, Scimeca, JA & Pariza, MW (1991) Mammary cancer prevention by conjugated linoleic acid. Cancer Research 51, 6118–6124.Google Scholar
Ip, C, Singh, M, Thompson, HJ & Scimeca, JA (1994) Conjugated linoleic acid suppresses mammary carcinogenesis and proliferative activity of the mammary gland in the rat. Cancer Research 54, 1212–1215.Google ScholarPubMed
Jahreis, G (1997) Krebshemmende Fettsäuren in Milch und Rindfleisch. Ernährungs-Umschau 44, 168–172.Google Scholar
Jahreis, G, Fritsche, J and & Steinhart, H (1996) Monthly variation of milk composition with special regard to fatty acids depending on season and farm management sytems – conventional versus ecological. Fett/Lipid 98, 356–359.CrossRefGoogle Scholar
Jensen, RG & Newburg, DC (1995) Bovine milk lipids. In Handbook of Milk Composition, pp. 543–575. [Jensen, RG, editors]. San Diego/CF: Academic Press.CrossRefGoogle Scholar
Jiang, J, Bjoerk, L, Fonden, R and & Emanuelson, M (1996) Occurrence of conjugated cis-9,trans-11-octadecadienoic acid in bovine milk: effects of feed and dietary regimen. Journal of Dairy Science 79, 438–445.CrossRefGoogle ScholarPubMed
Judd, JT, Clevidence, BA, Muesing, RA, Wittes, J, Sunkin, ME and & Podczasy, JJ (1994) Dietary trans fatty acids: effects on plasma lipids and lipoproteins of healthy men and women. American Journal of Clinical Nutrition 59, 861–868.CrossRefGoogle ScholarPubMed
Katan, MB, Zock, PL and & Mensink, RP (1994) Effects of fat and fatty acids on blood lipids in humans: an overview. American Journal of Clinical Nutrition 60, (Suppl), 1017S-1022S.CrossRefGoogle Scholar
Kelly, ML, Kolver, ES, Bauman, DE, Van Amburgh, ME & Muller, LD (1998) Effect of intake of pasture on concentration of conjugated linoleic acid in milk of lactating cows. Journal of Dairy Science 81, 1630–1636.CrossRefGoogle ScholarPubMed
Keys, A, Anderson, JT and & Grande, F (1965) Serum cholesterol response to changes in the diet. IV. Particular saturated fatty acids in the diet. Metabolism 14, 776–787.CrossRefGoogle ScholarPubMed
Kinsella, JE, Broughton, KS and & Whelan, JW (1990) Dietary unsaturated fatty acids: Interaction and possible needs in relation to eicosanoid synthesis. Journal of Nutritional Biochemistry 1, 123–141.CrossRefGoogle ScholarPubMed
Koletzko, B (1991) Zufuhr, Stoffwechsel und biologische Wirkungen trans-isomerer Fettsäuren bei Säuglingen. Nahrung 35, 229–283.CrossRefGoogle Scholar
Koletzko, B (1992) Trans fatty acids may impair biosynthesis of long-chain polyunsaturates and growth in man. Acta Pædiatrica 81, 302–306.CrossRefGoogle ScholarPubMed
Lagrost, L (1992) Differential effects of cis and trans fatty acid isomers, oleic and elaidic acid, on the cholesteryl ester transfer protein activity. Biochimica et Biophysica Acta 1124, 159–162.CrossRefGoogle ScholarPubMed
Lee, KN, Kritchewsky, D and & Pariza, MW (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 19–25.CrossRefGoogle ScholarPubMed
Lichtenstein, AH, Ausman, LM, Carrasco, W, Jenner, JL, Ordovas, JM and & Schaefer, EJ (1993) Hydrogenation impairs the hypolipidemic effect of corn oil in humans. Arteriosclerosis and Thrombosis 13, 154–161.CrossRefGoogle ScholarPubMed
Lin, H, Boylston, TD, Chang, MJ, Luedecke, LO & Shultz, TD (1995) Survey of the conjugated linoleic acid contents of dairy products. Journal of Dairy Science 78, 2358–2365.CrossRefGoogle ScholarPubMed
Marnett, LJ (1994) Generation of mutagens during arachidonic acid metabolism. Cancer Metastasis Reviews 13, 303–308.CrossRefGoogle ScholarPubMed
Mata, P, Garrido, JA, Ordovas, JM, Blazquez, E, Alvarez-Sala, LA, Rubio, MJ, Alonso, R, de Oya, M (1992) Effect of dietary monounsaturated fatty acids on plasma lipoproteins and apolipoproteins in women. American Journal of Clinical Nutrition 56, 77–83.CrossRefGoogle ScholarPubMed
Mattson, FH and & Grundy, SM (1985) Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man. Journal of Lipid Research 26, 194–202.CrossRefGoogle ScholarPubMed
Mensink, RP and & Katan, MB (1989) Effect of a diet enriched with monounsaturated or polyunsaturated fatty acids on levels of low-density and high-density lipoprotein cholesterol in healthy women and men. New England Journal of Medicine 321, 436–441.CrossRefGoogle ScholarPubMed
Mensink, RP and & Katan, MB (1990) Effect of dietary trans fatty acids on high-density and low-density lipoprotein cholesterol levels in healthy subjects. New England Journal of Medicine 323, 439–445.CrossRefGoogle ScholarPubMed
Mensink, RP, Zock, PL, Katan, MB and & Hornstra, G (1992) Effect of dietary cis and trans fatty acids on serum lipoprotein(a) levels in humans. Journal of Lipid Research 33, 1493–1501.CrossRefGoogle ScholarPubMed
Michal, JJ, Chew, BP, Shultz, TD, Wong, TS and & Magnuson, NS (1992) Interaction of conjugated dienoic derivates of linoleic acid with β-carotene on cellular host defence. FASEB Journal 6, A1102.Google Scholar
Miller, CC, Park, Y, Pariza, MW & Cook, ME (1994) Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biochemical and Biophysical Research Communications 198, 1107–1112.CrossRefGoogle ScholarPubMed
Molkentin, J and & Precht, D (1995) Optimized analysis of trans-octadecenoic acids in edible fats. Chromatographia 41, 267–272.CrossRefGoogle Scholar
Molkentin, J and & Precht, D (1997) Representative determination of the butyric acid content in European milk fats. Milchwissenschaft 52, 82–85.Google Scholar
Molkentin, J and & Precht, D (1997) Occurrence of trans-C16:1 acids in bovine milkfat and partially hydrogenated edible fats. Milchwissenschaft 52, 380–385.Google Scholar
Nestel, P, Noakes, M, Belling, B, McArthur, R, Clifton, P, Janus, E & Abbey, M (1992) Plasma lipoprotein lipid and Lp[a] changes with substitution of elaidic acid for oleic acid in the diet. Journal of Lipid Research 33, 1029–1036.CrossRefGoogle ScholarPubMed
Nicolosi, RJ, Rogers, EJ, Kritchevsky, D, Scimeca, JA & Huth, PJ (1997) Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery 22, 266–277.Google ScholarPubMed
Pariza, MW (1988) Dietary fat and cancer risk: evidence and research needs. Annual Review of Nutrition 8, 167–183.CrossRefGoogle ScholarPubMed
Park, Y, Albright, KJ, Liu, W, Storkson, JM, Cook, ME & Pariza, MW (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32, 853–858.CrossRefGoogle ScholarPubMed
Parodi, PW (1970) Fatty acid composition of Australian butter and milk fat. Australian Journal of Dairy Technology 25, 200–205.Google Scholar
Parodi, PW (1996) Milk fat components: possible chemopreventive agents for cancer and other diseases. Australian Journal of Dairy Technology 51, 24–32.Google Scholar
Parodi, PW (1997) Cow's milk fat components as potential anticarcinogenic agents. Journal of Nutrition 127, 1055–1060.CrossRefGoogle ScholarPubMed
Parodi, PW & Dunstan, RJ (1971) The trans unsaturated content of Queensland milk fats. Australian Journal of Dairy Technology 26, 60–62.Google Scholar
Parthasarathy, S and & Santanam, N (1994) Mechanisms of oxidation, antioxidants, and atherosclerosis. Current Opinion in Lipidology 5, 371–375.CrossRefGoogle ScholarPubMed
Pfeuffer, M (1997) Bedeutung mehrfach ungesättigter Fettsäuren in der Ernährung. In Fette in der Ernährung, Schriftenreihe des BML, Reihe A: Angewandte Wissenschaft, Heft 464, pp. 35–67 [Warwel, S and Weber, N, editors]. Bonn: Köllen-Verlag.Google Scholar
Pouillart, P, Cerutti, I, Ronco, G, Villa, P and & Chany, C (1991) Butyric monosaccharide ester-induced cell differentiation and antitumor activity in mice. Importance of their prolonged biological effect for clinical applications in cancer therapy. International Journal of Cancer 49, 89–95.CrossRefGoogle ScholarPubMed
Prasad, KN (1980) Butyric acid: a small fatty acid with diverse biological functions. Life Science 27, 1351–1358.CrossRefGoogle ScholarPubMed
Precht, D (1990) Quantitativer Nachweis von Milchfett in Schokoladenmischungen I: Bestimmung von Milchfettanteilen in Kakaobutter. Fat Science and Technology 92, 153–161.Google Scholar
Precht, D (1995) Variation of trans fatty acids in milk fats. Zeitschrift für Ernährungswissenschaft 34, 27–29.CrossRefGoogle ScholarPubMed
Precht, D, Frede, E, Hagemeister, H and & Timmen, H (1985) Zur fütterungsbedingten Variation des Milchfettes der Kuh unter besonderer Berücksichtigung von Regulationsmechanismen beim Fettstoffwechsel. Fette Seifen Anstrichmittel 87, 117–126.CrossRefGoogle Scholar
Precht, D and & Molkentin, J (1995) Trans fatty acids: Implications for health, analytical methods, incidence in edible fats and intake. Nahrung 39, 343–374.CrossRefGoogle ScholarPubMed
Precht, D and & Molkentin, J (1996) Rapid analysis of the isomers of trans-octadecenoic acid in milk fat. International Dairy Journal 6, 791–809.CrossRefGoogle Scholar
Precht, D and & Molkentin, J (1997) Trans-geometrical and positional isomers of linoleic acid including conjugated linoleic acid (CLA) in German milk and vegetable fats. Fett/Lipid 99, 319–326.CrossRefGoogle Scholar
Precht, D and & Molkentin, J (1997) Effect of feeding on conjugated cisΔ9,transΔ11-octadecadienoic acid and other isomers of linoleic acid in bovine milk fats. Nahrung 41, 330–335.CrossRefGoogle Scholar
Precht, D and & Molkentin, J (1997) Vergleich der Fettsäuren und der Isomerenverteilung der trans-C18:1-Fettsäuren von Milchfett, Margarine, Back-, Brat- und Diätfetten. Kieler Milchwirtschaftliche Forschungsberichte 49, 17–34.Google Scholar
Precht, D, Molkentin, J (1998) Correlations of anticarcinogenic conjugated linoleic acid with other C18 fatty acids in German bovine milk fat. In European Research Towards Safer and Better Food. Proceedings 3rd Karlsruhe Nutrition Symposium 1998. Part 2, pp. 150–158 [Gaukel, V and Spie&, WEL, editors]. Karlsruhe: Bundesanstalt für Ernährung..Google Scholar
Precht, D and & Molkentin, J (1999) Analysis and seasonal variation of conjugated linoleic acid and further cis-/trans-isomers of C18:1 and C18:2 in bovine milk fat. Kieler Milchwirtschaftliche Forschungsberichte 51, 63–78.Google Scholar
Rabizadeh, E, Shaklai, M, Nudelman, A, Eisenbach, L and & Rephaeli, A (1993) Rapid alteration of c-myc and c-jun expression in leukemic cells induced to differentiate by a butyric acid prodrug. FEBS Letters 328, 225–229.CrossRefGoogle ScholarPubMed
Reddy, BS (1994) Chemoprevention of colon cancer by dietary fatty acids. Cancer Metastasis Reviews 13, 285–302.CrossRefGoogle ScholarPubMed
Saito, H and & Ishihara, K (1997) Antioxidant activity and active sites of phospholipids as antioxidants. Journal of the American Oil Chemists' Society 74, 1531–1536.CrossRefGoogle Scholar
Shultz, TD, Chew, BP, Seaman, WR and & Luedecke, LO (1992) Inhibitory effects of conjugated dienoic derivatives of linoleic acid and beta-carotene on the in vitro growth of human cancer cells. Cancer Letters 63, 125–133.CrossRefGoogle ScholarPubMed
Siguel, EN and & Lerman, RH (1993) Trans fatty acid patterns in patients with angiographically documented coronary artery disease. American Journal of Cardiology 71, 916–920.CrossRefGoogle ScholarPubMed
Stanton, C, Lawless, F, Kjellmer, G, Harrington, D, Devery, R, Connolly, JF and & Murphy, J (1997) Dietary influences on bovine milk cis-9,trans-11-conjugated linoleic acid content. Journal of Food Science 62, 1083–1086.CrossRefGoogle Scholar
Valsta, LM, Jauhiainen, M, Aro, A, Katan, MB and & Mutanen, M (1992) Effects of a monounsaturated rapeseed oil and a polyunsaturated sunflower oil diet on lipoprotein levels in humans. Arteriosclerosis and Thrombosis 12, 50–57.CrossRefGoogle Scholar
Willett, WC, Stampfer, MJ, Manson, JE, Colditz, GA, Speizer, FE, Rosner, BA, Sampson, LA and & Hennekens, CH (1993) Intake of trans fatty acids and risk of coronary heart disease among women. Lancet 341, 581–585.CrossRefGoogle ScholarPubMed
Witztum, JL and & Steinberg, D (1991) Role of oxidized low density lipoprotein in atherogenesis. Journal of Clinical Investigation 88, 1785–1792.CrossRefGoogle ScholarPubMed
Wolff, RL (1994) Contribution of trans-18:1 acids from dairy fat to European diets. Journal of the American Oil Chemists' Society 71, 277–283.CrossRefGoogle Scholar
Wolff, RL and & Bayard, CC (1995) Improvement in the resolution of individual trans-18:1 isomers by capillary gas-liquid chromatography: use of a 100-m CP-Sil 88 column. Journal of the American Oil Chemists' Society 72, 1197–1201.CrossRefGoogle Scholar
Wood, R, Kubena, K, O'Brien, B, Tseng, S and & Martin, G (1993) Effect of butter, mono- and polyunsaturated fatty acid-enriched butter, trans fatty acid margarine, and zero trans fatty acid margarine on serum lipids and lipoproteins in healthy men. Journal of Lipid Research 34, 1–11.CrossRefGoogle ScholarPubMed
Zock, PL; 1995 Dietary fatty acids and risk factors for coronary heart disease: controlled studies in healthy volunteersPhD Thesis.Google Scholar
Zock, PL and & Katan, MB (1992) Hydrogenation alternatives: effects of trans fatty acids and stearic acid versus linoleic acid on serum lipids and lipoproteins in humans. Journal of Lipid Research 33, 399–410.CrossRefGoogle ScholarPubMed
Zöllner, N and, Tatò, F (1992) Fatty acid composition of the diet: impact on serum lipids and atherosclerosis. Clinical Investigation 70, 968–1009.Google ScholarPubMed