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Obesity induced during sexual maturation is linked to LDL-triacylglycerols in Yucatan miniature swine

Published online by Cambridge University Press:  08 March 2007

Sylvain P. Sébert ,
Gérard Lecannu ,
Sandrine Sené ,
Séverine Hucteau ,
Maud Chetiveaux ,
Khadija Ouguerram  and
Martine M.-J. Champ
Sylvain P. Sébert
Affiliation:
Human Nutrition and Gut Functions Unit, National Institute of Agronomic Research Rue de la Géraudière, BP 71627, 44316, Nantes, Cedex 3, France
Gérard Lecannu
Affiliation:
Human Nutrition and Gut Functions Unit, National Institute of Agronomic Research Rue de la Géraudière, BP 71627, 44316, Nantes, Cedex 3, France
Sandrine Sené
Affiliation:
Human Nutrition and Gut Functions Unit, National Institute of Agronomic Research Rue de la Géraudière, BP 71627, 44316, Nantes, Cedex 3, France
Séverine Hucteau
Affiliation:
Human Nutrition and Gut Functions Unit, National Institute of Agronomic Research Rue de la Géraudière, BP 71627, 44316, Nantes, Cedex 3, France
Maud Chetiveaux
Affiliation:
Human Nutrition Research Centre, CHU Hôtel Dieu Nantes, 1 place Alexis Ricordeau, 44093, Nantes, Cedex 1, France
Khadija Ouguerram
Affiliation:
Human Nutrition Research Centre, CHU Hôtel Dieu Nantes, 1 place Alexis Ricordeau, 44093, Nantes, Cedex 1, France
Martine M.-J. Champ*
Affiliation:
Human Nutrition and Gut Functions Unit, National Institute of Agronomic Research Rue de la Géraudière, BP 71627, 44316, Nantes, Cedex 3, France Human Nutrition Research Centre, CHU Hôtel Dieu Nantes, 1 place Alexis Ricordeau, 44093, Nantes, Cedex 1, France
*
*Corresponding author: Dr Martine Champ, fax +33 (0)2 40 67 51 12, email Champ@nantes.inra.fr
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Abstract

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The incidence of childhood obesity is rising dramatically throughout industrialised countries. To evaluate and study the impact of childhood obesity on lipoprotein metabolism, we developed a new animal model of premature obesity. Yucatan mini-pigs aged 4 months were studied over a 12-month period from childhood to adulthood. Animals were divided into two groups: the first group were overfed a Western misbalanced diet; the second group were normally fed a recommended human-type diet. Cholesterol and triacylglycerol concentrations in VLDL-, LDL- and HDL-lipoproteins were followed from baseline to adulthood by fast protein liquid chromatography. At 10 (the end of sexual maturation) and 16 months old (adulthood), liver, visceral and subcutaneous adipose tissues were sampled. Real-time RT-PCR was performed in order to compare apo AI, apo B, apo C-III, PPAR-α, insulin receptor and lipoprotein lipase gene expression between groups and ages. Differences between groups were observed only after sexual maturity. Adult overfed mini-pigs had a higher LDL-cholesterol:HDL-cholesterol ratio (P<0·05; 0·55 (SE 0·06) for overfed v. 0·42 (SE 0·04) for normally fed pigs at the tenth month of the study). In both groups, VLDL-triacylglycerol decreased (P<0·05). VLDL-triacylglycerol evolution in the overfed group was associated with an increase in LDL-triacylglycerol plasma concentrations (P<0·05) after sexual maturation. LDL-triacylglycerol concentration in overfed mini-pigs went from an average of 0·28 mmol/l before sexual maturation to reach an average concentration of 0·56 mmol/l afterwards. This phenomenon has never been observed in similar studies when obesity is induced in adult mini-pigs and may represent a specific hallmark of an obesity induced during sexual maturity.

Keywords

ObesityChildhoodAdulthoodLipoproteinsMini-pigs
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 2 , August 2005 , pp. 282 - 289
Copyright
Copyright © The Nutrition Society 2005

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