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Net flux of folates and vitamin B12 through the gastrointestinal tract and the liver of lactating dairy cows

Published online by Cambridge University Press:  09 March 2007

Christiane L. Girard ,
Hélène Lapierre ,
André Desrochers ,
Chaouki Benchaar ,
J. Jacques Matte  and
Didier Rémond
Christiane L. Girard*
Affiliation:
Agriculture and Agri-Food Canada, Lennoxville, Lennoxville, QC, Canada
Hélène Lapierre
Affiliation:
Agriculture and Agri-Food Canada, Lennoxville, Lennoxville, QC, Canada
André Desrochers
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, QC, Canada
Chaouki Benchaar
Affiliation:
Agriculture and Agri-Food Canada, Lennoxville, Lennoxville, QC, Canada Nova Scotia Agricultural College, Truro, NS, Canada
J. Jacques Matte
Affiliation:
Agriculture and Agri-Food Canada, Lennoxville, Lennoxville, QC, Canada
Didier Rémond
Affiliation:
Unité digestion et absorption des nutriments, INRA, St-Genés-Champanelle, France
*
*Corresponding author: Dr C. L. Girard, fax +1 819 564 5507, email girardch@em.agr.ca
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Abstract

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In study 1, four cows had a ruminal canula, a catheter in the right ruminal vein and an ultrasonic flow probe around the right ruminal artery; a catheter was placed in the auricular artery on experimental days. Blood samples were taken every 10 min from -20 to 60 min after ruminal infusion of 5·79 mmol pteroylmonoglutamic acid and cyanocobalamin. There was a net release of these vitamins across the rumen wall following the infusion (P=0·06). In studies 2 and 3, four cows had catheters in the portal, one hepatic and two mesenteric veins and one mesenteric artery. Plasma flow was determined using p-aminohippurate. In study 2, blood samples were taken before and every 30 min for 6 h after feeding 0 or 4 mg of pteroylmonoglutamic acid. Flow of folates through the portal-drained viscera (PDV) and the total splanchnic tissues (TSP) tended to increase with the ingestion of pteroylmonoglutamic acid (P=0·19). In study 3, blood samples were collected every 30 min for the first 3 h to calculate plasma flow and basal net fluxes of folates and vitamin B12. The cows were fed 2·6 g pteroylmonoglutamic acid and 500 mg cyanocobalamin; blood samples were taken every 2 h for 24 h. Vitamin supplements increased the net release of folates and vitamin B12 from PDV (P=0·04) and TSP (P=0·13). The present results demonstrate that, in dairy cows, at doses reported to improve animal performance, passage of pteroylmonoglutamic acid to the portal blood appears during the 6 h following its ingestion, whereas for cyanocobalamin, it is a slow process, not yet completed 24 h after its ingestion.

Keywords

Dairy cowFolic acidvitamin B12AbsorptionLiver
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 6 , December 2001 , pp. 707 - 715
Copyright
Copyright © The Nutrition Society 2001

References

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