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Metabolism of ornithine, α-ketoglutarate and arginine in isolated perfused rat liver

Published online by Cambridge University Press:  09 March 2007

Jean Pascal De Bandt ,
Luc Cynober ,
Soo Kyung Lim ,
Colette Coudray-Lucas ,
Raoul Poupon  and
Jacqueline Giboudeau
Jean Pascal De Bandt
Affiliation:
Laboratoire de Biochimie A, Paris, France
Luc Cynober
Affiliation:
Laboratoire de Biochimie A, Paris, France INSERM U 402 Hôpital Saint Antoine, Paris, France
Soo Kyung Lim
Affiliation:
Laboratoire de Biochimie A, Paris, France
Colette Coudray-Lucas
Affiliation:
Laboratoire de Biochimie A, Paris, France INSERM U 402 Hôpital Saint Antoine, Paris, France
Raoul Poupon
Affiliation:
INSERM U 402 Hôpital Saint Antoine, Paris, France
Jacqueline Giboudeau
Affiliation:
Laboratoire de Biochimie A, Paris, France
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Abstract

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Ornithine (Orn; α-ketoglutarate (αKG) salt) and arginine (Arg) supplementation of enteral diets has been advocated in the treatment of hypercatabolism of trauma patients, but both compounds are subject to extensive hepatic metabolism. To compare the metabolism of these two compounds and to evaluate the possible influence of the αKG moiety, livers were perfused with αKG, Orn, ornithine α-ketoglutarate (OKG) or Arg (n 6 in each group) for 1 h. Arg uptake was nearly fourfold higher than Orn uptake (690 (SD 162) ν. 178 (SD 30) nmol/min per g liver), and Orn uptake was not modified by αKG. Orn was totally metabolized by the liver, whereas Arg led to Orn release (408 (SD 159) nmol/min per g liver) and a threefold stimulation of urea production (Arg 1·44 (SD 0·22) ν. Orn 0·45 (SD 0.09) μol/min per g liver). αKG alone only increased hepatic aspartate uptake but, when associated with Orn as OKG, it led to an increase in giutamate release and in proiine content in the liver and to a decrease in proiine uptake. From these findings we conclude that (1) Arg load is extensively metabolized by the liver, inducing urea production, (2) in enteral use, Orn supplementation appears preferable to Arg as it is less ureogenic (as also recently demonstrated in vivo in stressed rats receiving isomolar amounts of Arg and Orn), (3) the liver participates in the Orn-αKG metabolic interaction, mostly in proiine metabolism, which occurs in the splanchnic area.

Keywords

Arginineα-KetoglutarateLiverOrnithine
Type
Hepatic metabolism in rats
Information
British Journal of Nutrition , Volume 73 , Issue 2 , February 1995 , pp. 227 - 239
Copyright
Copyright © The Nutrition Society 1995

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