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Lipid fluidity of triacylglycerol-rich lipoproteins isolated from copper-deficient rats *

Published online by Cambridge University Press:  09 March 2007

Claude Motta ,
Elyett Gueux ,
Andrzej Mazur  and
Yves Rayssiguier
Claude Motta
Affiliation:
Centre de Recherche en Nutrition Humaine de Clermont-Ferrand, Laboratoire de Biochimie, Hôtel Dieu, 63000 Clermont-Ferrand, France
Elyett Gueux
Affiliation:
Laboratoire des Maladies Métaboliques INRA, Theix 63122 St-Genès-Champanelle, France
Andrzej Mazur
Affiliation:
Laboratoire des Maladies Métaboliques INRA, Theix 63122 St-Genès-Champanelle, France
Yves Rayssiguier
Affiliation:
Laboratoire des Maladies Métaboliques INRA, Theix 63122 St-Genès-Champanelle, France
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Abstract

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Triacylglycerol-rich lipoproteins (TGRLP) were isolated from Cu-deficient and control rats. TGRLP from Cu-deficient rats appeared more fluid than those from controls as sensed by the fluorescence anisotropy of 1,6-diphenyl-l-3,5-hexatriene (DPH). This high fluidity was related to alow cholesterol: phospholipid ratio and high triacylglycerol content in these lipoproteins. TGRLP from Cu-deficient rats were more susceptible to in vitro peroxidation than lipoproteins from control rats as shown by the rate of diene conjugation. The damage induced by the peroxidation resulted in a more ordered state of the lipid fraction especially in lipoproteins from Cu-deficient rats. Thus, after in vitro peroxidation, TGRLP from Cu-deficient rats were more rigid than those from controls. These results suggest that Cu deficiency induces modifications in physicochemical properties of TGRLP which could affect their metabolism.

Keywords

CopperLipoproteinsPeroxidationLipid fluidity
Type
Copper deficiency and lipoprotein fluidity
Information
British Journal of Nutrition , Volume 75 , Issue 5 , May 1996 , pp. 767 - 773
Copyright
Copyright © The Nutrition Society 1996

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