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Inter- and intra-fat pad variation in vascularization and the release of 14C-labelled fatty acids in mice

Published online by Cambridge University Press:  02 April 2007

M. J. Gibney  and
J. Kearney
M. J. Gibney
Affiliation:
Division of Nutritional Sciences, Department of Clinical Medicine, Trinity College Medical School, St James‘ Hospital, Dublin 8, Republic of Ireland
J. Kearney
Affiliation:
Division of Nutritional Sciences, Department of Clinical Medicine, Trinity College Medical School, St James‘ Hospital, Dublin 8, Republic of Ireland
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Abstract

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Adult laca mice were dosed orally with 150 μl whole milk containing 2·5 μCi of either labelled stearic acid ([1-14C]18: 0; n 20) or labelled linoleic acid ([1 − 14C]18: 2; n 20). The mice were killed in groups of four at 6, 12, 24, 48 and 96 h following dosing and samples of perirenal and epididymal fat pads were taken from both inner and outer sites at each location. Significant differences in the rate of loss of label between sites were found. No differences (P = 0·018) between fat pad locations (epididymal v. perirenal) were found. A significant interaction between rate of loss of labelled fatty acid and site (P = 0·019) reflected the fact that between-site variations in this context were confined to labelled linoleic acid. In a second study weanling adult (n 10) and adult (n 10) mice were killed and their epididymal and perirenal fat pads prepared for histological examination. Both transverse and longitudinal sections were taken at the inner and outer sites of each fat pad location. Following staining, both the size and number of blood vessels were measured using computer-linked microscopy. In all instances there were significant differences between sites with the inner site consistently showing greater numbers and areas of blood vessels. In general the number of blood vessels in the inner site tended to be greater in older mice, while the reverse was seen for younger mice. The results lend support to the concept of multiple pools of triacylglycerol-fatty acids in adipose tissue such that the main determinant of shortterm supply of essential fatty acids is the quantity recently ingested.

Keywords

Adipose tissue turnoverFat pad vascularizationMice
Type
Lipid Metabolism
Information
British Journal of Nutrition , Volume 70 , Issue 3 , November 1993 , pp. 737 - 745
Copyright
Copyright © The Nutrition Society 1993

References

REFERENCES

American Institute of Nutrition. (1977). Report of the AIN ad hoe Committee on Standards for Nutritional Studies. Journal of Nutrition 107, 1340–1347.CrossRefGoogle Scholar
Angel, A. (1970). Studies on the compartmentation of lipid in adipose cells. 1. Subcellular distribution, composition and transport of newly synThesised lipid liposomes. Journal of Lipid Research 11, 420–432.CrossRefGoogle Scholar
Baker, N., Hill, V., Jacobson, M. & Bruckdorfer, K. R. (1984). Fatty acid metabolism in adipose tissue of ageing mice after direct tracer injection into fat pads. Mechanisms of Ageing and Development 27, 295–313.CrossRefGoogle ScholarPubMed
Belfrage, E., Hjemdore, P. & Fredholm, B. B. (1979). Metabolic effects of blood flow restrictions in adipose tissue. Acta Physiologrca Scandinavica 105, 222–227.CrossRefGoogle ScholarPubMed
Belfrage, P. (1985). Hormonal control of lipid degradation. In New Perspectives in Adipose Tissue: Structure, Function and Development, pp. 121–144. [Cryer, A. and Van, R. L. R., editors]. London: Butterworths.CrossRefGoogle Scholar
Blanchette-Mackie, E. J. & Scow, R. O. (1981). Membrane continuities within cells and intercellular contracts in white adipose tissue of young rats. Journal of Ultrastructure Research 77, 277–294..CrossRefGoogle Scholar
Burlow, J. & Madsen, J. (1981). Influence of blood flow on fatty acid mobilisation from lipolytically active adipose tissue. Pflügers Archives 390, 169–174.Google Scholar
Dayton, S., Hashimoto, S. & Dixon, W. (1966). Composition of lipids in human serum and adipose tissue during prolonged feeding of a diet high in unsaturated fat. Journal of Lipid Research 7, 103–111..CrossRefGoogle ScholarPubMed
Gellhorn, A. & Benjamin, W. (1965). Effects of aging on the composition and metabolism of adipose tissue in the rat. In Handbook of Physiology. Section 5. Adipose Tissue, pp. 399405. [Renold, A. and Cahill, G., editors]. Washington, DC: American Physiological Society.Google Scholar
Greenhouse, S. W. & Geisser, S. (1959). On methods in the analysis of profile data. Psychometrika 32, 95–112.CrossRefGoogle Scholar
Hamosh, M., Hamosh, P., Bar-maor, J. A. & Cohen, H. (1963). Fatty acid metabolism of human adipose tissue. Journal of Clinical Investigation 42, 1648–1652.CrossRefGoogle Scholar
Hartman, A. D. & Christ, D. W. (1978). Effect of cell size, age and anatomical location on the lipolytic response of adipocytes. Life Sciences 22, 1987–1996.CrossRefGoogle ScholarPubMed
Heimberg, M., Dunn, G. D. & Wilcox, H. G. (1974). The derivation of fatty acids from dietary neutral fat in man. Journal of Laboratory and Clinical Medicine 83, 393–402.Google ScholarPubMed
Hill, V. & Baker, N. (1983). Heterogenous labelling of adipocytes during in vivo-in vitro incubation of epididymal fat pads of ageing mice with 1-14C palmitate. Lipids 18, 25–31.CrossRefGoogle Scholar
Hirsch, J., Farquer, J. W., Ahrens, E. H., Peterson, M. L. & Stoffel, W. (1960). Studies of adipose tissue in man. A microtechnique for sampling and analysis. American Journal of Clinical Nutrition 8, 499–511.CrossRefGoogle Scholar
Hollenberg, C. H. & Vost, H. (1968). Regulation of DNA synThesis in fat cells and stromal elements from rat adipose tissue. Journal of Clinical Investigation 47, 2485–2498.CrossRefGoogle Scholar
Kearney, J., Kennedy, N. P., Keeling, P. W. N., Keating, J. J., Grubb, L., Kennedy, M. & Gibney, M. J. (1989). Dietary intakes and adipose tissue levels of linoleic acid in peptic ulcer disease. British Journal of Nutrition 62, 699–706.CrossRefGoogle ScholarPubMed
Kerpel, S., Shafrir, E. & Shapiro, B. (1961). Mechanisms of fatty acid assimilation in adipose tissue. Biochimica et Biophysicu Acta 46, 495–504.CrossRefGoogle ScholarPubMed
Klein, R. A., Halliday, D. & Pittet, P. G. (1980). The use of 13-methyl-tetradecanoic acid as an indicator of adipose tissue turnover. Lipids 15, 572–579.CrossRefGoogle Scholar
Kovach, A. G. B., Korach, E., Sandor, P., Spitzer, J. A. & Spitzer, J. J. (1976). Metabolic responses to localised ischaemia in adipose tissue. Journal of Surgical Research 20, 3744.CrossRefGoogle ScholarPubMed
Munkner, C. (1963). In vitro production of non-esterified fatty acids during the incubation of various types of adipose tissue from fasting and fed rats. 1. The mobilisation of fatty acids from epididymal, perinephric and subcutaneous adipose tissue. Scandinavian Journal of Clinicul and Laboratory Investigation 15, 83–90.CrossRefGoogle Scholar
Ookthens, M., Montisano, D., Lyon, I. & Baker, N. (1987). Transport and metabolism of extracellular free fatty acids in adipose tissue of fed and fasted mice. Journal of Lipid Research 28, 528–539.CrossRefGoogle Scholar
Statistical Analysis Systems (1985). SAS Users Guide: Statistics, version 5. Cary, NC: SAS Institute Inc.Google Scholar
Small, D. M. (1991). The effects of glyceride structure on absorption and metabolism. Annual Review of Nutrition 11, 413–434.CrossRefGoogle ScholarPubMed
Smith, U. (1985). Regional differences in adipocyte metabolism and possible consequences in vivo. International Journal of Obesiiy 9, 145–148.Google Scholar
Stein, Y. & Stein, O. (1962). Metabolic activity in rat epididymal fat pad labelled selectively by an in vivo incubation technique. Biochimica el Biophysica Aria 54, 555–571.CrossRefGoogle Scholar
Vernon, R. G. & Clegg, R. A. (1985). The metabolism of white adipose tissue in vivo and in vitro. In New Perspectives in Adipose Tissue: Structure, Function and Developmenr, pp. 65–86. [Cryer, A. and Van, R. L. R., editors]. London: Butterworths.CrossRefGoogle Scholar
Winand, G., Furnelle, J., Wodon, J. C. & Christophe, J. (1971). Spectrum of fatty acids synThesised in situ and metabolic heterogeneity of free fatty acids and glycerides within isolated rat adipocytes. Biochimica et Biophysicu Acta 239, 142–153.CrossRefGoogle ScholarPubMed
Wood, D. A.Butler, S. & Riemersma, R. A. (1984). Adipose tissue and platelet fatty acids and coronary heart disease in Scottish men. Lancet ii, 117–121.CrossRefGoogle Scholar
Zinder, O., Eisenberg, E. & Shapiro, B. (1973). Compartmentation of glycerides in adipose tissue cells. Journal of Biological Chemistry 248, 7673–7676.CrossRefGoogle ScholarPubMed