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Influence of diets containing different natural oils on the incorporation of [1-14C]acetate in the various lipid fractions of rat liver

Published online by Cambridge University Press:  09 March 2007

E. P. M. Bhattathiry
E. P. M. Bhattathiry
Affiliation:
Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
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Abstract

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1. A comparative study was undertaken with rats on the effect of various diets (normal stock, fat-free, palm oil and olive oil) on the in vitro incorporation of [14C]acetate by the liver into cholesterol and into the fatty acids of phospholipids and neutral fats. 2. The total lipids extracted from the incubation mixtures were fractionated into acetone-precipitable and digi- tonin-precipitable portions and also into the fatty acids of neutral lipids. 3. The incorporation of [14C]acetate into the acetone-precipitable fraction and into fatty acids of neutral fats was greatest in livers of rats given the fat-free diet, followed by those of the groups given olive oil, the normal stock diet, and palm oil. Livers from the group given the fat-free diet also exhibited the highest percentage of 14C activity in the digitonin-precipitable fraction and were closely followed by the group on the normal stock diet. Compared with those of the other two groups, the livers of the groups given olive oil and palm oil showed much less activity in the digitonin- precipitable fraction. 4. The greater the amount of a specific type of fatty acid in the diet, the less was the 14C activity incorporated into that type of fattyacid in the ncutral fats of liver slices, hut this was not so with the fatty acids obtained froin the acetone-precipitahlc fraction of the lipids.

Type
Research Article
Information
British Journal of Nutrition , Volume 20 , Issue 3 , September 1966 , pp. 553 - 559
Copyright
Copyright © The Nutrition Society 1966

References

REFERENCES

Bernhard, K. & Steinhauser, H. (1944). Helv. chim. Acta 27, 207.CrossRefGoogle Scholar
Boxer, G. E. & Stetten, D. Jr. (1944). J. biol. Chem. 153, 607.CrossRefGoogle Scholar
Brice, E. G., Okey, R. & Stone, M. M. (1956). J. biol. Chem. 218, 107.CrossRefGoogle Scholar
Burchfield, H. P. & Stores, E. E. (1962). Biochemical Applications of Gas-chromatography, p. 494. New York: Academic Press Inc.Google Scholar
Chase, G. D. & Rabinowitz, J. L. (1963). Principles of Radioisotope Methodology, p. 187. Minneapolis: Burgess Publishing Co.Google Scholar
Dawson, R. M. C., Hemington, N. & Davenport, J. B. (1962). Biochem. J. 84, 497.CrossRefGoogle Scholar
De Boer, T. J. & Backer, H. J. (1956). Org. Synth. 36, 16.Google Scholar
De Vries, (1963). J. Am. Oil. Chem. Soc. 40, 184.CrossRefGoogle Scholar
Enser, M. & Bartley, W. (1962). Biochem. J. 85, 607.CrossRefGoogle Scholar
Folch, J., Lees, M. & Stanley, G. H. S. (1957). J. biol. Chem. 226, 497.CrossRefGoogle Scholar
Haven, F. L. & Bloor, W. R. (1956). Adv. cancer Res. 4, 237.CrossRefGoogle Scholar
Hegsted, D. M., Whyman, C., Gotsis, A. & Andrews, S. A. (1960). J. clin. Nutr. 8, 209.CrossRefGoogle Scholar
Hill, R., Linazasoro, J. M., Chevallier, F. & Chaikoff, I. L. (1958). J. biol. Chem. 233, 305.CrossRefGoogle Scholar
Kline, D., McPherson, C., Pritchard & Rossiter, R. J. (1956 a). J. biol. Chem. 222, 219.CrossRefGoogle Scholar
Kline, D., McPherson, C., Pritchard & Rossiter, R. J. (1956 b). Proc. Soc. exp. Biol. Med. 92, 756.CrossRefGoogle Scholar
Masoro, E. J. (1962). J. Lipid Res. 3, 149.CrossRefGoogle Scholar
Schlenk, H. & Getterman, J. L. (1960). Analyt. Chem. 32, 1412.CrossRefGoogle Scholar
Sperry, W. M. & Webb, M. (1950). J. biol. Chem. 187, 97.CrossRefGoogle Scholar
Tischer, K., Opalka, E. & Glenn, J. L. (1963). Fedn Proc. Fedn Am. Socs exp. Biol. 22, 377.Google Scholar
Whitney, J. E. & Roberts, S. (1955). Am. J. Physiol. 181, 446.CrossRefGoogle Scholar