Hostname: page-component-76fb5796d-9pm4c Total loading time: 0 Render date: 2024-04-25T16:11:07.727Z Has data issue: false hasContentIssue false
  • English
  • Français

In vivo measurement of lipogenesis in ruminants using [1-14C]acetate

Published online by Cambridge University Press:  09 March 2007

H. M. R. Greathead ,
J. M. Dawson ,
N. D. Scollan  and
P. J. Buttery
H. M. R. Greathead*
Affiliation:
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK
J. M. Dawson
Affiliation:
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK
N. D. Scollan
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, UK
P. J. Buttery
Affiliation:
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK
*
*Corresponding author: Dr Henry Greathead, present address Centre for Animal Sciences, School of Biology, University of Leeds, Leeds LS2 9JT, UK, fax +44 113 2333066, email H.M.R.Greathead@leeds.ac.uk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A method for the measurement of the rate of lipogenesis in ruminants using a continuous intravenous infusion of [1-14C]acetate and measuring the rate of [1-14C]acetate incorporation into adipose tissue lipid was evaluated. Subcutaneous adipose tissue samples obtained by biopsy over the course of a 6 h continuous intravenous infusion of [1-14C]acetate into a wether and a steer maintained in a ‘metabolic steady state’ demonstrated that the incorporation of [1-14C]acetate into subcutaneous adipose tissue lipid was linear for the duration of the infusion period. Subsequent measures of rates of [1-14C]acetate incorporation into adipose tissue lipid were made on adipose tissue samples taken at a single time point during the infusion period. The technique was used to measure rates of lipogenesis in the subcutaneous adipose tissue of fourteen Hereford × Friesian steers that had been fed a pelleted diet of dried grass at a range of metabolizable energy (ME) intakes from 1·1 × ME requirement for maintenance to ad libitum for 11 weeks. Rates of lipogenesis increased linearly (P<0·001) with increasing ME intake. It was concluded that the method is an effective technique for measuring rates of lipogenesis in specific adipose tissue depots in vivo in ruminants.

Keywords

RuminantLipogenesisAcetateMetabolizable energy intake
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 1 , July 2001 , pp. 37 - 44
Copyright
Copyright © The Nutrition Society 2001

References

Agriculture and Food Research Council (1993) Energy and Protein Requirements of Ruminants. Wallingford: CAB INTERNATIONAL.Google Scholar
Annison, EF (1993) Fat deposition in ruminants. In Recent Advances in Animal Nutrition in Australia pp. 51–60 [Farrell, DJ, editor]. Armidale, New South Wales, Austraila: University of New England.Google Scholar
Agriculture Research Council (1980) The Nutrient Requirements of Ruminant Livestock. Slough: Commonwealth Agricultural Bureau.Google Scholar
Bauman, DE (1976) Intermediary metabolism of adipose tissue. Federation Proceedings 35, 2308–2313.Google ScholarPubMed
Berg, RT & Walters, LE (1983) The meat animal – changes and challenges. Journal of Animal Science 57, 133–146.Google Scholar
Broad, TE & Ulyatt, MJ (1980) The effect of level of food intake on the incorporation of acetate into lipids and its distribution among various tissues in sheep. British Journal of Nutrition 44, 71–79.CrossRefGoogle ScholarPubMed
Cronjé, PB, Nolan, JV & Leng, RA (1991) Acetate clearance rate as a potential index of the availability of glucogenic precursors in ruminants fed on roughage-based diets. British Journal of Nutrition 66, 301–312.CrossRefGoogle ScholarPubMed
Davey, HW (1986) Measurement of fat synthesis in sheep. MSc Thesis University of Canterbury, New Zealand.Google Scholar
Dawson, JM, Greathead, HMR, Sessions, VA, Tye, FM & Buttery, PJ (1999) Effect of gastric inhibitory polypeptide on bovine fat metabolism. Comparative Biochemistry and Physiology 123, 79–88.CrossRefGoogle ScholarPubMed
Dole, VP & Meinertz, H (1960) Microdetermination of long-chain fatty acids in plasma and tissues. Journal of Biological Chemistry 235, 2595–2599.CrossRefGoogle ScholarPubMed
Dunshea, FR, Harris, DM, Bauman, DE, Boyd, RD & Bell, AW (1992) Effect of porcine somatotropin on in vivo glucose kinetics and lipogenesis in growing pigs. Journal of Animal Science 70, 141–151.CrossRefGoogle ScholarPubMed
England, P & Gill, M (1983) The effect of wilting and short-chopping of grass on the subsequent voluntary intake of silage, and live-weight gain of calves. Animal Production 36, 73–77.Google Scholar
Ingle, DL, Bauman, DE & Garrigus, US (1972 a) Lipogenesis in the ruminant: in vitro study of tissue site, carbon source and reducing equivalent generation for fatty acid synthesis. Journal of Nutrition 102, 609–616.CrossRefGoogle Scholar
Ingle, DL, Bauman, DE & Garrigus, US (1972 b) Lipogenesis in the ruminant: in vivo site of fatty acid synthesis in sheep. Journal of Nutrition 102, 617–624.CrossRefGoogle ScholarPubMed
Madsen, A (1983) The molecular basis of animals production: metabolism in skeletal muscle cells. In Dynamic Biochemistry of Animal Production, pp. 9–28 [Riis, P, editor]. Amsterdam: Elsevier.Google Scholar
Mersmann, HJ (1986) Comparison of plasma free-fatty-acid and blood-glycerol concentrations with measurement of lipolysis in porcine adipose tissue in vitro. Journal of Animal Science 63, 757–769.CrossRefGoogle ScholarPubMed
Mills, SE, Lemenager, RP & Horstman, LA (1989) Adipose tissue lipogenesis in growing steers adapted to different levels of feed intake. Journal of Animal Science 67, 3011–3017.CrossRefGoogle ScholarPubMed
Persson, M, Bleiberg, B, Kiss, D & Miles, J (1991) Measurement of plasma acetate kinetics using high-performance liquid chromatography. Analytical Biochemistry 198, 149–153.CrossRefGoogle ScholarPubMed
Pond, CM (1992) An evolutionary and functional view of mammalian adipose-tissue. Proceedings of the Nutrition Society 51, 367–377.CrossRefGoogle ScholarPubMed
Pond, CM (1999) Physiological specialisation of adipose tissue. Progress in Lipid Research 38, 225–248.CrossRefGoogle ScholarPubMed
Smith, SB, Prior, RL, Koong, LJ & Mersmann, HJ (1992) Nitrogen and lipid metabolism in heifers fed at increasing levels of intake. Journal of Animal Science 70, 152–160.CrossRefGoogle ScholarPubMed
Vernon, RG (1981) Lipid metabolism in the adipose tissue of ruminant animals. In Lipid Metabolism in Ruminants, pp. 279–362 [Christie, W, editor]. Oxford: Pergamon Press.CrossRefGoogle Scholar
Vernon, RG (1992) Control of lipogenesis and lipolysis. In The Control of Fat and Lean Deposition, pp. 59–81 [Buttery, P, Boorman, K and Linsay, D, editors]. Oxford: Butterworth Heinemann Ltd.CrossRefGoogle Scholar
Waterlow, J, Garlick, P & Millward, D (editors) (1978) Protein Turnover in Mammalian Tissues and in the Whole Body. Amsterdam: North Holland.Google Scholar