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H2OAthletes study protocol: effects of hydration changes on neuromuscular function in athletes

Published online by Cambridge University Press:  01 February 2024

Rúben Francisco*
Affiliation:
Exercise and Health Laboratory, CIPER, Faculdade Motricidade Humana, Universidade de Lisboa, 1499–002 Cruz-Quebrada, Lisbon, Portugal
Filipe Jesus
Affiliation:
Exercise and Health Laboratory, CIPER, Faculdade Motricidade Humana, Universidade de Lisboa, 1499–002 Cruz-Quebrada, Lisbon, Portugal
Catarina L. Nunes
Affiliation:
Exercise and Health Laboratory, CIPER, Faculdade Motricidade Humana, Universidade de Lisboa, 1499–002 Cruz-Quebrada, Lisbon, Portugal
Paulo Santos
Affiliation:
Neuromuscular Research Lab, CIPER, Faculdade Motricidade Humana, Universidade de Lisboa, Lisbon, Portugal
Marta Alvim
Affiliation:
National Institute of Health Doutor Ricardo Jorge, Lisbon, Portugal
Francesco Campa
Affiliation:
Department of Biomedical Sciences, University of Padua, Padua, Italy
Dale A. Schoeller
Affiliation:
Interdepartmental Graduate Program in Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, USA
Henry Lukaski
Affiliation:
Department of Kinesiology and Public Health Education, Hyslop Sports Center, University of North Dakota, Grand Forks, ND, USA
Goncalo V. Mendonca
Affiliation:
Neuromuscular Research Lab, CIPER, Faculdade Motricidade Humana, Universidade de Lisboa, Lisbon, Portugal
Luís Fernando Cordeiro Bettencourt Sardinha
Affiliation:
Exercise and Health Laboratory, CIPER, Faculdade Motricidade Humana, Universidade de Lisboa, 1499–002 Cruz-Quebrada, Lisbon, Portugal
Analiza Mónica Lopes de Almeida Silva
Affiliation:
Exercise and Health Laboratory, CIPER, Faculdade Motricidade Humana, Universidade de Lisboa, 1499–002 Cruz-Quebrada, Lisbon, Portugal
*
*Corresponding author: Rúben Francisco, email rubenfrancisco@fmh.ulisboa.pt

Abstract

We aim to understand the effects of hydration changes on athletes’ neuromuscular performance, on body water compartments, fat-free mass hydration and hydration biomarkers and to test the effects of the intervention on the response of acute dehydration in the hydration indexes. The H2OAthletes study (clinicaltrials.gov ID: NCT05380089) is a randomised controlled trial in thirty-eight national/international athletes of both sexes with low total water intake (WI) (i.e. < 35·0 ml/kg/d). In the intervention, participants will be randomly assigned to the control (CG, n 19) or experimental group (EG, n 19). During the 4-day intervention, WI will be maintained in the CG and increased in the EG (i.e. > 45·0 ml/kg/d). Exercise-induced dehydration protocols with thermal stress will be performed before and after the intervention. Neuromuscular performance (knee extension/flexion with electromyography and handgrip), hydration indexes (serum, urine and saliva osmolality), body water compartments and water flux (dilution techniques, body composition (four-compartment model) and biochemical parameters (vasopressin and Na) will be evaluated. This trial will provide novel evidence about the effects of hydration changes on neuromuscular function and hydration status in athletes with low WI, providing useful information for athletes and sports-related professionals aiming to improve athletic performance.

Type
Protocol Paper
Copyright
© The Author(s), 2024. Published by Cambridge University Press on behalf of The Nutrition Society

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