Hostname: page-component-5c6d5d7d68-wtssw Total loading time: 0 Render date: 2024-08-06T20:32:25.553Z Has data issue: false hasContentIssue false
  • English
  • Français

Glycosyl ureides in ruminant nutrition

1. Preparation and estimation of lactosyl urea and other glycosyl ureides

Published online by Cambridge University Press:  09 March 2007

R. J. Merry ,
R. H. Smith  and
A. B. McAllan
R. J. Merry
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
R. H. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
A. B. McAllan
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Glucosyl urea, lactosyl urea and galactosyl urea were prepared from pure sugars and urea and their purity confirmed by determination of their melting points, specific rotations and by mass spectrometry.

2. Using whey as a lactose source, a range of conditions were examined for the preparation of lactosyl urea on a laboratory scale. Yields of 60% were achieved when lactose and urea (molar ratio, urea: lactose 0:6) were reacted for 15 h in sulphuric acid at pH 2·0 and a temperature of 70°.

3. Methods of detection and estimation of all three ureides in whey preparations and ruminant digesta samples were developed. Two quantitative methods, one involving acid-hydrolysis, the other ion-exchange chromatography, were used. The latter method enabled determination of individual quantities of ureides in mixtures.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 48 , Issue 2 , September 1982 , pp. 275 - 286
Copyright
Copyright © The Nutrition Society 1982

References

REFERENCES

Al Attar, A., Evans, R. A. & Axford, R.F.E. (1976). Proc. Nutr. Soc. 35, 108A.Google Scholar
Balch, C. C. & Porter, J. W. G. (1977). Symp. zool. Soc. Lond. no. 48, 285.Google Scholar
Bartley, E. E. & Deyoe, C. W. (1977). In Recent Advances in Animal Nutrition, p. 50 [Haresign, W. and Lewis, D., editors]. London: Butterworths.Google Scholar
Benn, M. H. & Jones, A. S. (1960). J. chem. Soc. p. 3837.CrossRefGoogle Scholar
Cerbulis, J., Pfeffer, P. E. & Farrell, H. M. (1978). Carbohydr. Res. 65, 311.CrossRefGoogle Scholar
Chalupa, W. & Davis, R. F. (1976). Proc. Mld Nutr. Conf. Fd Mfrs, University of Maryland, p. 6.Google Scholar
Conway, E. J. (1957). Microdiffusion Analysis and Volumetric Error, 4th ed. London: Crosby Lockwood.Google Scholar
Demeyer, D. & Van Nevel, C. (1978). Revue Agric. Brux. 31, 1094.Google Scholar
Galyamin, N. L. (1975). Zhivotnovodstvo. 9, 30.Google Scholar
Gehrke, C. W. & Leimer, K. (1971). J. Chromat. 57, 219.CrossRefGoogle Scholar
Goodman, I. (1958). Adv. Carbohydr. chem. 13, 215.Google Scholar
Gouda, I., Larm, O. & Larsson, K. (1980). Swedish J. agric. Res. 10, 97.Google Scholar
Gregory, M. E. (1954). Br. J. Nutr. 8, 340.CrossRefGoogle Scholar
Hynd, A. (1926). Biochem. J. 20, 205.CrossRefGoogle Scholar
Lin, A. Y. & Nickerson, T. A. (1976). J. Dairy Sci. 60, 34.CrossRefGoogle Scholar
McAllan, A. B., Merry, R. J. & Smith, R. H. (1975). Proc. Nutr. Soc. 34, 90A.Google Scholar
McAllan, A. B. & Smith, R. H. (1974). Br. J. Nutr. 31, 77.CrossRefGoogle Scholar
Merry, R. J. (1980). The use of dietary non-protein nitrogen compounds by the ruminant with particular emphasis on the glycosyl ureides. PhD Thesis, University of Reading.Google Scholar
Milligan, L. P., Worsley, M., Elofson, M., Young, B. A. & Atwal, A. S. (1972). J. Anim. Sci. 34, 89A.Google Scholar
National Academy of Sciences (1976). Urea and Other Non-protein Nitrogen Compounds in Animal Nutrition. Washington, DC: National Research Council.Google Scholar
Oldham, J. D., Buttery, P. J., Swan, J. & Lewis, D. (1977). J. agric. Sci., Camb. 89, 467.CrossRefGoogle Scholar
Schingoethe, D. J. (1976). J. Dairy Sci. 59, 556.CrossRefGoogle Scholar
Schoorl, M. N. (1903). Recl. Trav. chim. Pays-Bas, 22, 31.CrossRefGoogle Scholar
Smith, R. H. (1979). J. Anim. Sci. 49, 1604.CrossRefGoogle Scholar
Smith, R. H. & McAllan, A. B. (1971). Automation in Analytical Chemistry (Technicon International Symposium, 1969), p. 207. Basingstoke: Technicon Instruments Co. Ltd.Google Scholar
Smith, R. H., Salter, D. N. & Daneshvar, K. (1977). J. Nuclear agric. Biol. 6, 8.Google Scholar
Technicon Instruments Co. Ltd (1967). Methodology sheet N-1c. New York: Technicon.Google Scholar
Thivend, P. (1977). Wld. Anim. Rev. 23, 20.Google Scholar
Trevelyan, W. E., Proctor, D. F. & Harrison, J. S. (1950). Nature, Lond. 166, 444.CrossRefGoogle Scholar
Widell, S. (1979). Proc. Whey Products Conf., Minneapolis, 1978, p. 53. Whey Products Institute and USDA.Google Scholar