Hostname: page-component-7bb8b95d7b-l4ctd Total loading time: 0 Render date: 2024-09-11T11:15:12.099Z Has data issue: false hasContentIssue false
  • English
  • Français

Experiments with the long-term rumen simulation technique (Rusitec); use of soluble food and an inert solid matrix

Published online by Cambridge University Press:  09 March 2007

J. W. Czerkawski  and
Grace Breckenridge
J. W. Czerkawski
Affiliation:
The Hannah Research Institute, Ayr, Scotland KA6 5HL
Grace Breckenridge
Affiliation:
The Hannah Research Institute, Ayr, Scotland KA6 5HL
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The role of soluble nutrients and of the solid matrix in rumen fermentation was investigated in some detail, and experiments designed to explore the possibility of using a balanced soluble diet and an inert solid matrix, are described.

2. The use of a balanced soluble substrate as the only source of nutrients in the presence of an inert solid phase in the reaction vessels results in vigorous fermentation but is accompanied by disappearance of protozoa from the effluent.

3. In the absence of digestible solid phase, the rate of fermentation and the fermentation pattern depends mainly on the amount and type of nutrients supplied and to a smaller extent on the dilution rate, the variations being greatest at low dilution rates.

4. The solid matrix in the form of wood shavings or the residue remaining after prolonged digestion of hay could sequestrate micro-organisms and could be used as solid phase, but the defined mixture of soluble substrates used resulted in somewhat abnormal fermentation compared with fermentation obtained with solid food.

5. When the solid food included some hay extract and when the dilution rate was not too great a reduced output of protozoa could be maintained. At high dilution rate the outflow of protozoa was negligible and yet considerable numbers of protozoa were found in the solid matrix and associated liquid.

6. A three-compartment model was developed to describe the flow of liquid and microbial matter within the simplified system.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 42 , Issue 2 , September 1979 , pp. 229 - 245
Copyright
Copyright © The Nutrition Society 1979

References

Czerkawski, J. W. (1976). J. Sci. Fd Agric. 27, 323.CrossRefGoogle Scholar
Czerkawski, J. W. & Breckenridge, G. (1969). Br. J. Nutr. 23, 559.CrossRefGoogle Scholar
Czerkawski, J. W. & Breckenridge, G. (1977). Br. J. Nutr. 38, 371.CrossRefGoogle Scholar
Czerkawski, J. W. & Breckenridge, G. (1979). Br. J. Nutr. 4, 217.CrossRefGoogle Scholar
Latham, M. J., Brooker, B. E., Pettipher, G. L. & Harris, P. J. (1978). Appl. Environ. Microbiol. 35, 1166.CrossRefGoogle Scholar
McDougall, E. I. (1948). Biochem. J. 43, 99.CrossRefGoogle Scholar
Marty, R. J. & Demeyer, D. I. (1973). Br. J. Nutr. 30, 369.CrossRefGoogle Scholar
Orpin, C. G. & Letcher, A. J. (1978). J. gen. Microbiol. 106, 33.CrossRefGoogle Scholar