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Erythrocytes, erythrocyte membranes, neutrophils and platelets as biopsy materials for the assessment of zinc status in humans

Published online by Cambridge University Press:  09 March 2007

Manuel Ruz ,
Kelley R. Cavan ,
William J. Bettger  and
Rosalind S. Gibson
Manuel Ruz
Affiliation:
Division of Applied Human Nutrition and Department of Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Kelley R. Cavan
Affiliation:
Division of Applied Human Nutrition and Department of Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
William J. Bettger
Affiliation:
Division of Applied Human Nutrition and Department of Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Rosalind S. Gibson
Affiliation:
Division of Applied Human Nutrition and Department of Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
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Abstract

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During a controlled zinc depletion-repletion study, fifteen men aged 25.3 (sd 3.3) years were fed on a low-Zn diet with high phytate:Zn and phytate × calcium: Zn molar ratios for 7 weeks, followed by a 2 week repletion period when 30 mg supplemental Zn/d was given. Changes in plasma, urine, and hair Zn concentrations, taste acuity, and cellular immune response confirmed the development of mild Zn deficiency. Zn concentrations in neutrophils, platelets, erythrocytes and erythrocyte membranes, mean platelet volume, and activities of alkaline phosphatse (EC 3.1.3.1) and α-d-mannosidase (EC 3.2.1.24) in neutrophils did not respond to changes in Zn status. In contrast, alkaline phosphatase activity in erythrocyte membranes showed a significant decline which was consistent in all subjects (nmol product formed/min per mg protein; baseline v. 7-week Zn depletion, 0.656 (sd 0.279) v. 0.506 (sd 0.230), at 7 weeks; P < 0.05); neutral phosphatase activity remained unchanged. Alkaline phosphatase activity in erythrocyte membranes may be a potential index of Zn status in humans

Keywords

Experimental zinc deficiencyHumansPhytateNeutrophilsPlatelets
Type
Metabolic Effects of Altered Zinc Status
Information
British Journal of Nutrition , Volume 68 , Issue 2 , September 1992 , pp. 515 - 527
Copyright
Copyright © The Nutrition Society 1992

References

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