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Endogenous purine and pyrimidine derivative excretion in pregnant sows

Published online by Cambridge University Press:  09 March 2007

S. M. Martin Orue ,
J. Balcells ,
J. A. Guada  and
C. Castrillo
S. M. Martin Orue
Affiliation:
Departamento de Productión Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza, 50013, Spain
J. Balcells
Affiliation:
Departamento de Productión Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza, 50013, Spain
J. A. Guada
Affiliation:
Departamento de Productión Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza, 50013, Spain
C. Castrillo
Affiliation:
Departamento de Productión Animal y Ciencia de los Alimentos, Facultad Veterinaria, Miguel Servet 177, Zaragoza, 50013, Spain
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Abstract

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The present experiment was carried out to study the endogenous losses of purine and pyrimidine derivatives from pregnant sows. Three pregnant and three non-pregnant Large White × Landrace sows were fed on a purine-free diet composed of starch, glucose, sucrose and vegetable oil, with casein as the protein source. The experiment began, for the six animals, after diagnosis of pregnancy and was divided into six 12 d periods. Urine was collected during the first 3 d of each experimental period by means of a urethral catheter for determination of allantoin, uric acid, xanthine, hypoxanthine and pseudouridine concentrations. In the absence of dietary nucleic acids (N A), allantoin and, as a consequence, excretion of total purine derivatives (PD) decreased significantly to a constant value (128·3 (se 7·07) μmol/kg metabolic live weight (W0·7) per d), an amount assumed to represent endogenous excretion. Excretion of uric acid (38·7 (SE 2·15) μmol/kg W0·75 per d), hypoxanthine (21·0 (SE 2·58) μmol/kg W0·75 per d) and xanthine (11·2 (SE 0·83) μmol/kg W0·75 per d) were not affected by the experimental treatment, although there was a significant decrease in hypoxanthine excretion in pregnant sows (from 25·5 to 5·2 μmol/kg W0·75 per d) compared with non-pregnant sows (from 26·7 to 44·8 μmol/kg W0·75 per d). Creatinine excretion was not affected by pregnancy and was used as an internal urinary marker. Purine excretion, either expressed as μmol/kg W0·75 per d or as the ratio PD:creatinine, was not affected by experimental treatment, although an apparent increase in pseudouridine excretion, a modified unsalvageable catabolite of RNA-pyrimidine, was found in late pregnancy (3·6 ν 52 mol/100 mol creatinine in on-pregnant sows compared with pregnant sows at 102 d collection).

Keywords

Purine derivativesPseudouridinePregnancySow
Type
Pregnancy and purine metabolism in sows
Information
British Journal of Nutrition , Volume 73 , Issue 3 , March 1995 , pp. 375 - 385
Copyright
Copyright © The Nutrition Society 1995

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