Hostname: page-component-77c89778f8-9q27g Total loading time: 0 Render date: 2024-07-17T05:53:29.688Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of age, and protein malnutrition followed by a balanced diet on the non-parallel change in digestive enzymes in the pancreas and their secretion in the rat

Published online by Cambridge University Press:  09 March 2007

Josiane Prost ,
Jacques Belleville  and
Carole Valantin-Rollet
Josiane Prost
Affiliation:
Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 – 21004 Dijon Cedex, France
Jacques Belleville
Affiliation:
Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 – 21004 Dijon Cedex, France
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Ninety male Wistar rats were divided into two groups. A control group (C) was fed on a balanced diet, containing 200 g protein/kg for 51 d. An experimental group (E) was fed on a low-protein diet containing 50 g protein/kg for 28 d (PM), and then on a balanced diet for 23 d (BR). At different days of PM and BR, the pancreas and the pancreatic juice were collected 40 min after injection of 0.1 mCi [3H]leucine. The amounts of amylase (EC 3.2.1.1), trypsinogen 2 (EC 3.4.21.4), chymotrypsinogen 1 (EC 3.4.21.1) and lipase (EC 3.1.1.3) were determined after separation by the isoelectric focussing technique. Incorporation of [3H]leucine into the four hydrolases of pancreatic juice and pancreas was also determined.

2. In control rats a progressive increase in the concentration of digestive enzymes and the amounts secreted were observed with age. Maturation was reached when the rats were 9 weeks old. In rats E, PM inhibited maturation of the pancreas. However, individual enzymes were not affected to the same extent and at the same time. As soon as re-feeding was initiated, pancreas maturation took place and a significant increase in these variables was observed. The increases varied according to the hydrolase and did not appear at the same time.

3. In control rats, a preferential secretion of newly synthesized enzymes was observed in young rats, whereas with age, the proportion of newly synthesized enzymes excreted decreased slowly. In group E rats, at the beginning of PM, the proportion of newly synthesized enzymes secreted was very low and increased with time.

4. In rats C and E, our results indicated a non-parallelism between pancreatic enzyme levels and amounts secreted. This non-parallelism was different in both groups, it was changed with age and pancreas maturation in group C, and according to nutritional state in group E.

Type
General Nutrition papers
Information
British Journal of Nutrition , Volume 60 , Issue 3 , November 1988 , pp. 619 - 631
Copyright
Copyright © The Nutrition Society 1988

References

Adelson, J. W. & Rothman, S. S. (1974) Science 183, 1087–1089.Google Scholar
Borgström, B. & Hildebrand, H. (1975) Scandinavian Journal of Gastroenterology 10, 585–591.Google Scholar
Dagorn, J. C. (1978) Journal of Physiology 280, 435–448.CrossRefGoogle Scholar
Dagorn, J. C., Paradis, D. & Morisset, J. (1977 a) Digestion 15, 110–121.CrossRefGoogle Scholar
Dagorn, J. C., Sahel, J. & Sarles, H. (1977 b) Gastroenterology 73, 42–45.Google Scholar
Figarella, C., Taulier, J. & Sarles, H. (1965) Bulletin de la Société de Chimie Biologique 47, 679–686.Google Scholar
Gardner, J. D. & Jensen, R. T. (1980) American Journal of Physiology 238, G63–G86.Google Scholar
Giorgi, D., Renaud, W., Bernard, J. P. & Dagorn, J. C. (1985) Biochemical and Biophysical Research Communications 127, 937–942.Google Scholar
Grossman, M. I., Greengard, H. & Ivy, A. C. (1942) American Journal of Physiology 138, 676–682.CrossRefGoogle Scholar
Howard, F. & Yudkin, J. (1963) British Journal of Nutrition 17, 281–294.CrossRefGoogle Scholar
Ishe, I. & Lilja, P. (1979) Science 204, 1212–1215.Google Scholar
Jarnieson, J. D. & Palade, G. E. (1971) Journal of Cell Biology SO, 135–158.CrossRefGoogle Scholar
Keim, V. (1986). Annals of Nutrition and Metabolism 30, 104–112, 113–119.CrossRefGoogle Scholar
Kheroua, O. & Belleville, J. (1981) Reproduction, Nutrition, Développemenf 21, 901–918.Google Scholar
Leung, Y. K., Lee, P. C. & Lebenthal, E. (1986) American Journal of Physiology 250, G594–G597.Google Scholar
Lowry, O. H., Rosenbrough, W. J., Farr, A. L. & Randall, R. J. (1951) Journal of Biological Chemistry 193, 265–275.Google Scholar
MacLeod, E. P., Derbenwick, J. P. & Snook, J. T. (1972) Journal of Nutrition 102, 469–478.Google Scholar
Marchis Mouren, G. (1965) Bulletin de la Société de Chimie Biologique 41, 2207–2217.Google Scholar
Moore, P. A. (1980) Analytical Biochemistry 108, 151–155.Google Scholar
Palade, G. E. (1975) Science 189, 247–258.CrossRefGoogle Scholar
Prost, J. & Belleville, J. (1987) Journal de Physiologie 349, 53A.Google Scholar
Prost, J., Belleville, J. & Bouziane, M. (1982) Bulletin de la Société de Biologie 175, 690–699.Google Scholar
Prost, J., Belleville, J. & Bouziane, M. (1983) Bulletin de la Société de Biologie 177, 488–502.Google Scholar
Robberecht, P., Cremer, M. & Christophe, J. (1977) Gastroenterology 72, 417–420.CrossRefGoogle Scholar
Rothman, S. S. & Isenman, L. D. (1974) American Journal of Physiology 226, 1082–1087.Google Scholar
Saraux, B., Girard-Globa, A., Ouagued, M. & Vacher, D. (1982) American Journal of Physiology 243, G10–G15.Google Scholar
Scheele, G. & Palade, G. E. (1975) Journal of Biological Chemistry 250, 2660–2670.CrossRefGoogle Scholar
Schick, J., Verspohl, R., Kern, H. & Scheele, G. (1984) American Journal of Physiology 247, G611–G616.Google Scholar
Simoes-Nunes, C., Corring, T. & Chayvialle, J. A. (1981) Reproduction, Nutrition, Développement 21, 809.Google Scholar
Street, H. V. & Close, J. R. (1956) Clinica Chimica Acta 1, 256–268.CrossRefGoogle Scholar
Uriel, J. (1971). In Method of Immunochemistry, Vol. 3, pp. 29k–321. [Williams, C.A., Chase, M. W., editors]. New York and London: Academic Press.Google Scholar
Van Nest, G. A., MacDonald, R. J., Raman, R. K. & Rutter, W. J. (1980) Journal of Cell Biology 87, 784–794.Google Scholar
Watanabe, T., Ogasavara, N., Goto, H. & Yamada, Y. (1980) Febs Letters 121, 369–371.CrossRefGoogle Scholar
Waterlow, J. C. (1975). In Protein-Calorie Malnutrition, pp. 23–25. [Olson, R. E., editor]. New York and San Francisco: Academic Press.CrossRefGoogle Scholar
Wicker, C., Puigserver, A. & Scheele, G. (1984) European Journal of Biochemistry 139, 381–387.Google Scholar